LOCUS BBa_K1472613 3384 bp DNA linear 22-Nov-2024 DEFINITION ∆9, ∆12, ∆15 desaturases with LacI promoter ACCESSION BBa_K1472613 VERSION BBa_K1472613.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 227..229 /label=Start misc_feature 1181..1186 /label=Stop CDS 227..1186 /label=BBa_K1027001 misc_feature 1199..1202 /label=conserved RBS 1195..1206 /label=BBa_B0034 misc_feature 1213..1215 /label=Start misc_feature 2269..2274 /label=Stop CDS 1213..2274 /label=BBa_K1027002 misc_feature 2287..2290 /label=conserved RBS 2283..2294 /label=BBa_B0034 CDS 2301..3404 /label=Delta 15 desaturase CDS 2301..3384 /label=BBa_K1472610 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc tgaatccgct 241 gaatattgaa tatctgtacc tgagcaaact gttcgataat agcctgatcg tgtttaacaa 301 acgtcagctg tttcgttttt ttgtgcgctt ttttttcatg accgcagcac tgccgaatga 361 tagcaaaccg aaactgacac cggcatggac cgtgattttt ttctttacca gcattcatct 421 ggttgcactg ctggcatttc tgccgcagtt ttttagctgg aaagcagttg gtatggcctt 481 tctgctgtat gttattaccg gtggtattgg tattaccctg ggttttcatc gttgtattag 541 ccatcgtagc tttaatgttc cgaaatggct ggaatatatc tttgttattt gtggcaccct 601 ggcctgtcag ggtggtgttt ttgaatgggt tggtctgcat cgtatgcatc acaaatttag 661 cgataccaca ccggatccgc atgatagcaa taaaggtttt tggtggtcac atattggctg 721 gatgatgttt gaaattccgg caaaagcaga tattccgcgt tataccaaag atatccagga 781 cgataaattc taccagttct gccagaataa cctgattctg attcaggttg cactgggtct 841 gattctgttt gccctgggtg gttggccgtt tgttatttgg ggtatttttg ttcgtctggt 901 gtttgtgttt cactttacct ggtttgttaa tagcgccacc cataaatttg gttatgttag 961 ccatgagagc aacgattata gccgtaattg ttggtgggtt gccctgctga cctttggtga 1021 aggttggcat aataatcatc acgcctatca gtatagcgca cgtcatggtc tgcaatggtg 1081 ggaagttgat ctgacctgga tgaccatcaa atttctgagc ctgctgggtc tggcaaaaga 1141 tattaaactg cctccggaaa ccgcaatggc aaataaagcc taataatact agagaaagag 1201 gagaaatact agatgaccgc aaccattccg cctctgaccc cgaccgttac cccgagcaat 1261 ccggatcgtc cgattgcaga tctgaaactg caagatatta tcaaaaccct gccgaaagaa 1321 tgcttcgaaa aaaaagcaag caaagcatgg gcaagcgttc tgattaccct gggtgcaatt 1381 gcagttggtt atctgggtat tatctatctg ccgtggtatt gtctgccgat tacctggatt 1441 tggaccggca ccgcactgac cggtgcattt gttgttggtc atgattgtgg tcatcgtagc 1501 tttgcaaaaa aacgttgggt taatgatctg gtgggtcata ttgcatttgc accgctgatt 1561 tatccgtttc atagctggcg tctgctgcat gatcatcatc atctgcatac caacaaaatc 1621 gaagttgata atgcatggga tccgtggtca gttgaagcat ttcaggcaag tccggcaatt 1681 gttcgtctgt tttatcgtgc aattcgtggt ccgttttggt ggaccggtag catttttcat 1741 tggagcctga tgcattttaa actgagcaat tttgcacagc gcgatcgcaa taaagtgaaa 1801 ctgagcattg cagtggtttt tctgtttgca gcaattgcat ttccggcact gattattacc 1861 accggtgttt ggggttttgt gaaattttgg ctgatgccgt ggctggttta tcatttttgg 1921 atgagcacct ttaccatcgt gcatcatacc attccggaaa ttcgttttcg tccggcagca 1981 gattggagcg cagccgaagc acagctgaat ggcaccgttc attgtgatta tccgcgttgg 2041 gttgaagttc tgtgccatga tattaatgtg catattccgc atcatctgag cgttgcaatt 2101 ccgagctata atctgcgtct ggcacatggt agcctgaaag aaaattgggg tccgtttctg 2161 tatgaacgca cctttaattg gcagctgatg cagcagatta gcggtcagtg tcatctgtat 2221 gatccggaac atggttatcg tacctttggc agcctgaaaa aagtgtaata atgatactag 2281 agaaagagga gaaatactag atgcgtctgg aaattagcag tccgcagacc aaactgccgt 2341 atccgaaaac cgaagaactg ccgtttaccc tgcaagaact gcgtaatgca attccggcag 2401 attgttttga accgagcgtt gttcgtagcc tgggttattt ctttctggat gttggtctga 2461 ttgcaggttt ttatgcactg gcagcatatc tggatagctg gtttttctat ccgatttttt 2521 ggctgattca gggcaccctg ttttggagcc tgtttgttgt tggtcatgat tgtggtcatg 2581 gtagctttag caaaagcaaa accctgaata attggattgg tcatctgagc cataccccga 2641 ttctggttcc gtatcatggt tggcgtatta gccatcgtac ccatcatgca aataccggta 2701 atattgatac cgatgaaagc tggtatccgg ttagcgaaca gaaatataac cagatggcat 2761 ggtatgaaaa actgctgcgt ttttatctgc cgctgattgc atatccgatt tacctgtttc 2821 gtcgtagccc gaatcgtcag ggtagccatt ttatgcctgg tagtccgctg tttcgtccgg 2881 gtgaaaaagc agcagttctg accagcacct ttgccctggc agcatttgtt ggttttctgg 2941 gctttctgac ctggcagttt ggttggctgt ttctgctgaa attctatgtt gcaccgtatc 3001 tggtttttgt tgtttggctg gatctggtta cctttctgca tcataccgaa gataacattc 3061 cgtggtatcg tggtgatgat tggtattttc tgaaaggtgc actgagcacc attgatcgtg 3121 attatggttt tatcaacccg atccatcatg atattggcac ccatgttgca catcatatct 3181 ttagcaatat gccgcactat aaactgcgtc gtgcaaccga agcaattaaa ccgattctgg 3241 gtgaatatta tcgttatagc gacgaaccga tttggcaggc atttttcaaa agctattggg 3301 cctgtcattt tgttccgaat cagggtagcg gtgtttatta tcagagcccg agcaatggtg 3361 gttatcagaa aaaaccgtaa taat //