BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961222 1 BBa_R0010 range1961222 1 1 200 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1027001 1 BBa_K1027001 Delta 9 Desaturase, optimised for expression in Escherichia coli 2013-09-18T11:00:00Z 2015-05-08T01:08:46Z Synthesized gene based off the protein sequence from Synechocystis sp This enzyme is obtained fro the gene Stearoly Co-A Desaturase (SCD). The gene is obtained from Synechocystis sp., a cyanobacterium.It is involved in the biosynthesize of C18:1 from C18:0. C18:1 corresponds to Oleic acid. The delta 9 desaturase introduces cis carbon double bond on the 9th carbon in C18:0. false false _1334_ 0 15689 9 In stock true Codon optimized for expression in E. coli false Robert Harrison, Lorna Hepworth, Vun Hiang Tan annotation2357079 1 Stop range2357079 1 955 960 annotation2357078 1 Start range2357078 1 1 3 BBa_K1027002 1 BBa_K1027002 Delta 12 Desaturase, optimised for expression in Escherichia coli 2013-09-18T11:00:00Z 2015-05-08T01:08:46Z Produced synthetically based on protein sequence from Synechocystis sp. This enzyme is obtained from the gene Oleic acid desaturase (ode).The gene is obtained from Synechocystis sp., a cyanobacterium and introduced in the E.coli. It is a trans-membrane enzyme, involved in the biosynthesis of C18:2 from C18:1. C18:2 corresponds to Linolenic acid. The delta 12 desaturase introduces a second cis carbon double bond on the 12th carbon in C18:1. false false _1334_ 0 15689 9 In stock true Codon optimization for expression in E coli false Robert Harrison, Lorna Hepworth, Vun Hiang Tan annotation2354834 1 Start range2354834 1 1 3 annotation2354835 1 Stop range2354835 1 1057 1062 BBa_K1472613 1 BBa_K1472613 &#8710;9, &#8710;12, &#8710;15 desaturases with LacI promoter 2014-11-01T12:00:00Z 2015-05-08T01:10:39Z All the desaturase sequences are derived from the Synechocystis sp. but optimised for the expression in E. coli. This protein generator is composed of the delta 9, delta 12 and delta 15 desaturase genes with an RBS upstream of each coding sequence and LacI regulated promoter. Desaturases introduce cis-carbon double bonds to the carbon chain of fatty acids. &#8710;9 desaturase introduces a double bond to stearic acid between carbon 9 and carbon 10 to produce oleic acid. &#8710;12 desaturase introduces a double bond between carbon 12 and carbon 13 of oleic acid so to produce linoleic acid. In the end, &#8710;15 desaturase converts linoleic acid into alpha-linolenic acid by adding a double bond between carbon 15 and carbon 16 (Figure 1). false false _1851_ 0 22971 9 Not in stock false The desaturase genes in plasmids were introduced into E. coli for amplification. The final construct includes iGEM provided RBS (BBa_B0034). false Lau Tsz Kit component2429818 1 BBa_K1027002 component2429813 1 BBa_K1027001 component2429810 1 BBa_B0034 component2429802 1 BBa_R0010 component2429822 1 BBa_K1472610 component2429815 1 BBa_B0034 component2429820 1 BBa_B0034 annotation2429810 1 BBa_B0034 range2429810 1 209 220 annotation2429822 1 BBa_K1472610 range2429822 1 2301 3384 annotation2429818 1 BBa_K1027002 range2429818 1 1213 2274 annotation2429815 1 BBa_B0034 range2429815 1 1195 1206 annotation2429802 1 BBa_R0010 range2429802 1 1 200 annotation2429813 1 BBa_K1027001 range2429813 1 227 1186 annotation2429820 1 BBa_B0034 range2429820 1 2283 2294 BBa_K1472610 1 BBa_K1472610 Delta 15 desaturase 2014-09-27T11:00:00Z 2015-05-08T01:10:39Z The amino acid sequence of delta 15 desaturase is derived from Synechocystis sp. but codon optimised for the expression in E. coli. This sequence encoded for the delta15 desaturase. The gene is from Synechocystis sp. and optimised for the expression in E. coli. During the fatty acid conversion process, delta 15 desaturase catalyses the conversion of linolenic acid (18:2) into alpha-Linolenic acid, ALA (18:3) by adding a cis-doube bond at carbon 15. false false _1851_ 0 23007 9 In stock false The enzyme cut site must be designed according to iGEM prefix and suffix requirements. false Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu annotation2390390 1 Delta 15 desaturase range2390390 1 1 1104 BBa_K1472610_sequence 1 atgcgtctggaaattagcagtccgcagaccaaactgccgtatccgaaaaccgaagaactgccgtttaccctgcaagaactgcgtaatgcaattccggcagattgttttgaaccgagcgttgttcgtagcctgggttatttctttctggatgttggtctgattgcaggtttttatgcactggcagcatatctggatagctggtttttctatccgattttttggctgattcagggcaccctgttttggagcctgtttgttgttggtcatgattgtggtcatggtagctttagcaaaagcaaaaccctgaataattggattggtcatctgagccataccccgattctggttccgtatcatggttggcgtattagccatcgtacccatcatgcaaataccggtaatattgataccgatgaaagctggtatccggttagcgaacagaaatataaccagatggcatggtatgaaaaactgctgcgtttttatctgccgctgattgcatatccgatttacctgtttcgtcgtagcccgaatcgtcagggtagccattttatgcctggtagtccgctgtttcgtccgggtgaaaaagcagcagttctgaccagcacctttgccctggcagcatttgttggttttctgggctttctgacctggcagtttggttggctgtttctgctgaaattctatgttgcaccgtatctggtttttgttgtttggctggatctggttacctttctgcatcataccgaagataacattccgtggtatcgtggtgatgattggtattttctgaaaggtgcactgagcaccattgatcgtgattatggttttatcaacccgatccatcatgatattggcacccatgttgcacatcatatctttagcaatatgccgcactataaactgcgtcgtgcaaccgaagcaattaaaccgattctgggtgaatattatcgttatagcgacgaaccgatttggcaggcatttttcaaaagctattgggcctgtcattttgttccgaatcagggtagcggtgtttattatcagagcccgagcaatggtggttatcagaaaaaaccgtaataat BBa_K1027001_sequence 1 atgctgaatccgctgaatattgaatatctgtacctgagcaaactgttcgataatagcctgatcgtgtttaacaaacgtcagctgtttcgtttttttgtgcgcttttttttcatgaccgcagcactgccgaatgatagcaaaccgaaactgacaccggcatggaccgtgatttttttctttaccagcattcatctggttgcactgctggcatttctgccgcagttttttagctggaaagcagttggtatggcctttctgctgtatgttattaccggtggtattggtattaccctgggttttcatcgttgtattagccatcgtagctttaatgttccgaaatggctggaatatatctttgttatttgtggcaccctggcctgtcagggtggtgtttttgaatgggttggtctgcatcgtatgcatcacaaatttagcgataccacaccggatccgcatgatagcaataaaggtttttggtggtcacatattggctggatgatgtttgaaattccggcaaaagcagatattccgcgttataccaaagatatccaggacgataaattctaccagttctgccagaataacctgattctgattcaggttgcactgggtctgattctgtttgccctgggtggttggccgtttgttatttggggtatttttgttcgtctggtgtttgtgtttcactttacctggtttgttaatagcgccacccataaatttggttatgttagccatgagagcaacgattatagccgtaattgttggtgggttgccctgctgacctttggtgaaggttggcataataatcatcacgcctatcagtatagcgcacgtcatggtctgcaatggtgggaagttgatctgacctggatgaccatcaaatttctgagcctgctgggtctggcaaaagatattaaactgcctccggaaaccgcaatggcaaataaagcctaataa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K1027002_sequence 1 atgaccgcaaccattccgcctctgaccccgaccgttaccccgagcaatccggatcgtccgattgcagatctgaaactgcaagatattatcaaaaccctgccgaaagaatgcttcgaaaaaaaagcaagcaaagcatgggcaagcgttctgattaccctgggtgcaattgcagttggttatctgggtattatctatctgccgtggtattgtctgccgattacctggatttggaccggcaccgcactgaccggtgcatttgttgttggtcatgattgtggtcatcgtagctttgcaaaaaaacgttgggttaatgatctggtgggtcatattgcatttgcaccgctgatttatccgtttcatagctggcgtctgctgcatgatcatcatcatctgcataccaacaaaatcgaagttgataatgcatgggatccgtggtcagttgaagcatttcaggcaagtccggcaattgttcgtctgttttatcgtgcaattcgtggtccgttttggtggaccggtagcatttttcattggagcctgatgcattttaaactgagcaattttgcacagcgcgatcgcaataaagtgaaactgagcattgcagtggtttttctgtttgcagcaattgcatttccggcactgattattaccaccggtgtttggggttttgtgaaattttggctgatgccgtggctggtttatcatttttggatgagcacctttaccatcgtgcatcataccattccggaaattcgttttcgtccggcagcagattggagcgcagccgaagcacagctgaatggcaccgttcattgtgattatccgcgttgggttgaagttctgtgccatgatattaatgtgcatattccgcatcatctgagcgttgcaattccgagctataatctgcgtctggcacatggtagcctgaaagaaaattggggtccgtttctgtatgaacgcacctttaattggcagctgatgcagcagattagcggtcagtgtcatctgtatgatccggaacatggttatcgtacctttggcagcctgaaaaaagtgtaataatga BBa_K1472613_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgctgaatccgctgaatattgaatatctgtacctgagcaaactgttcgataatagcctgatcgtgtttaacaaacgtcagctgtttcgtttttttgtgcgcttttttttcatgaccgcagcactgccgaatgatagcaaaccgaaactgacaccggcatggaccgtgatttttttctttaccagcattcatctggttgcactgctggcatttctgccgcagttttttagctggaaagcagttggtatggcctttctgctgtatgttattaccggtggtattggtattaccctgggttttcatcgttgtattagccatcgtagctttaatgttccgaaatggctggaatatatctttgttatttgtggcaccctggcctgtcagggtggtgtttttgaatgggttggtctgcatcgtatgcatcacaaatttagcgataccacaccggatccgcatgatagcaataaaggtttttggtggtcacatattggctggatgatgtttgaaattccggcaaaagcagatattccgcgttataccaaagatatccaggacgataaattctaccagttctgccagaataacctgattctgattcaggttgcactgggtctgattctgtttgccctgggtggttggccgtttgttatttggggtatttttgttcgtctggtgtttgtgtttcactttacctggtttgttaatagcgccacccataaatttggttatgttagccatgagagcaacgattatagccgtaattgttggtgggttgccctgctgacctttggtgaaggttggcataataatcatcacgcctatcagtatagcgcacgtcatggtctgcaatggtgggaagttgatctgacctggatgaccatcaaatttctgagcctgctgggtctggcaaaagatattaaactgcctccggaaaccgcaatggcaaataaagcctaataatactagagaaagaggagaaatactagatgaccgcaaccattccgcctctgaccccgaccgttaccccgagcaatccggatcgtccgattgcagatctgaaactgcaagatattatcaaaaccctgccgaaagaatgcttcgaaaaaaaagcaagcaaagcatgggcaagcgttctgattaccctgggtgcaattgcagttggttatctgggtattatctatctgccgtggtattgtctgccgattacctggatttggaccggcaccgcactgaccggtgcatttgttgttggtcatgattgtggtcatcgtagctttgcaaaaaaacgttgggttaatgatctggtgggtcatattgcatttgcaccgctgatttatccgtttcatagctggcgtctgctgcatgatcatcatcatctgcataccaacaaaatcgaagttgataatgcatgggatccgtggtcagttgaagcatttcaggcaagtccggcaattgttcgtctgttttatcgtgcaattcgtggtccgttttggtggaccggtagcatttttcattggagcctgatgcattttaaactgagcaattttgcacagcgcgatcgcaataaagtgaaactgagcattgcagtggtttttctgtttgcagcaattgcatttccggcactgattattaccaccggtgtttggggttttgtgaaattttggctgatgccgtggctggtttatcatttttggatgagcacctttaccatcgtgcatcataccattccggaaattcgttttcgtccggcagcagattggagcgcagccgaagcacagctgaatggcaccgttcattgtgattatccgcgttgggttgaagttctgtgccatgatattaatgtgcatattccgcatcatctgagcgttgcaattccgagctataatctgcgtctggcacatggtagcctgaaagaaaattggggtccgtttctgtatgaacgcacctttaattggcagctgatgcagcagattagcggtcagtgtcatctgtatgatccggaacatggttatcgtacctttggcagcctgaaaaaagtgtaataatgatactagagaaagaggagaaatactagatgcgtctggaaattagcagtccgcagaccaaactgccgtatccgaaaaccgaagaactgccgtttaccctgcaagaactgcgtaatgcaattccggcagattgttttgaaccgagcgttgttcgtagcctgggttatttctttctggatgttggtctgattgcaggtttttatgcactggcagcatatctggatagctggtttttctatccgattttttggctgattcagggcaccctgttttggagcctgtttgttgttggtcatgattgtggtcatggtagctttagcaaaagcaaaaccctgaataattggattggtcatctgagccataccccgattctggttccgtatcatggttggcgtattagccatcgtacccatcatgcaaataccggtaatattgataccgatgaaagctggtatccggttagcgaacagaaatataaccagatggcatggtatgaaaaactgctgcgtttttatctgccgctgattgcatatccgatttacctgtttcgtcgtagcccgaatcgtcagggtagccattttatgcctggtagtccgctgtttcgtccgggtgaaaaagcagcagttctgaccagcacctttgccctggcagcatttgttggttttctgggctttctgacctggcagtttggttggctgtttctgctgaaattctatgttgcaccgtatctggtttttgttgtttggctggatctggttacctttctgcatcataccgaagataacattccgtggtatcgtggtgatgattggtattttctgaaaggtgcactgagcaccattgatcgtgattatggttttatcaacccgatccatcatgatattggcacccatgttgcacatcatatctttagcaatatgccgcactataaactgcgtcgtgcaaccgaagcaattaaaccgattctgggtgaatattatcgttatagcgacgaaccgatttggcaggcatttttcaaaagctattgggcctgtcattttgttccgaatcagggtagcggtgtttattatcagagcccgagcaatggtggttatcagaaaaaaccgtaataat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z