LOCUS BBa_K1473009 5011 bp DNA linear 23-Dec-2024 DEFINITION pRha induced CRISPR ACCESSION BBa_K1473009 VERSION BBa_K1473009.1 FEATURES Location/Qualifiers misc_feature 1..325 /label=tracrRNA part of BBa_K1218011 misc_feature 341..462 /label=BBa_K914003 misc_feature 472..5011 /label=dCas9 + CRISPR array of BBa_K1218011 ORIGIN 1 ttacgaaatc atcctgtgga gcttagtagg tttagcaaga tggcagcgcc taaatgtaga 61 atgataaaag gattaagaga ttaatttccc taaaaatgat aaaacaagcg ttttgaaagc 121 gcttgttttt ttggtttgca gtcagagtag aatagaagta tcaaaaaaag caccgactcg 181 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat gctgttttga 241 atggttccaa caagattatt ttataacttt tataacaaat aatcaaggag aaattcaaag 301 aaatttatca gccataaaac aatacccgcg ggagttcgat ccacaattca gcaaattgtg 361 aacatcatca cgttcatctt tccctggttg ccaatggccc attttcctgt cagtaacgag 421 aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg aaatcacccg tatggataag 481 aaatactcaa taggcttaga tatcggcaca aatagcgtcg gatgggcggt gatcactgat 541 gaatataagg ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc 601 aaaaaaaatc ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt 661 ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag 721 gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag 781 tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta 841 gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta 901 gattctactg ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag 961 tttcgtggtc attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa 1021 ctatttatcc agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca 1081 agtggagtag atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa 1141 aatctcattg ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct 1201 ttgtcattgg gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa 1261 ttacagcttt caaaagatac ttacgatgat gatttagata atttattggc gcaaattgga 1321 gatcaatatg ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca 1381 gatatcctaa gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa 1441 cgctacgatg aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt 1501 ccagaaaagt ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt 1561 gatgggggag ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg 1621 gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg 1681 acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg 1741 agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc 1801 ttgacttttc gtattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca 1861 tggatgactc ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat 1921 aaaggtgctt cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca 1981 aatgaaaaag tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa 2041 ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa 2101 cagaagaaag ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa 2161 ttaaaagaag attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt 2221 gaagatagat ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat 2281 aaagattttt tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg 2341 accttatttg aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt 2401 gatgataagg tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct 2461 cgaaaattga ttaatggtat tagggataag caatctggca aaacaatatt agattttttg 2521 aaatcagatg gttttgccaa tcgcaatttt atgcagctga tccatgatga tagtttgaca 2581 tttaaagaag acattcaaaa agcacaagtg tctggacaag gcgatagttt acatgaacat 2641 attgcaaatt tagctggtag ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt 2701 gttgatgaat tggtcaaagt aatggggcgg cataagccag aaaatatcgt tattgaaatg 2761 gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga 2821 atcgaagaag gtatcaaaga attaggaagt cagattctta aagagcatcc tgttgaaaat 2881 actcaattgc aaaatgaaaa gctctatctc tattatctcc aaaatggaag agacatgtat 2941 gtggaccaag aattagatat taatcgttta agtgattatg atgtcgatca cattgttcca 3001 caaagtttcc ttaaagacga ttcaatagac aataaggtct taacgcgttc tgataaaaat 3061 cgtggtaaat cggataacgt tccaagtgaa gaagtagtca aaaagatgaa aaactattgg 3121 agacaacttc taaacgccaa gttaatcact caacgtaagt ttgataattt aacgaaagct 3181 gaacgtggag gtttgagtga acttgataaa gctggtttta tcaaacgcca attggttgaa 3241 actcgccaaa tcactaagca tgtggcacaa attttggata gtcgcatgaa tactaaatac 3301 gatgaaaatg ataaacttat tcgagaggtt aaagtgatta ccttaaaatc taaattagtt 3361 tctgacttcc gaaaagattt ccaattctat aaagtacgtg agattaacaa ttaccatcat 3421 gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa 3481 cttgaatcgg agtttgtcta tggtgattat aaagtttatg atgttcgtaa aatgattgct 3541 aagtctgagc aagaaatagg caaagcaacc gcaaaatatt tcttttactc taatatcatg 3601 aacttcttca aaacagaaat tacacttgca aatggagaga ttcgcaaacg ccctctaatc 3661 gaaactaatg gggaaactgg agaaattgtc tgggataaag ggcgagattt tgccacagtg 3721 cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt acagacaggc 3781 ggattctcca aggagtcaat tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa 3841 aaagactggg atccaaaaaa atatggtggt tttgatagtc caacggtagc ttattcagtc 3901 ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt taaagagtta 3961 ctagggatca caattatgga aagaagttcc tttgaaaaaa atccgattga ctttttagaa 4021 gctaaaggat ataaggaagt taaaaaagac ttaatcatta aactacctaa atatagtctt 4081 tttgagttag aaaacggtcg taaacggatg ctggctagtg ccggagaatt acaaaaagga 4141 aatgagctgg ctctgccaag caaatatgtg aattttttat atttagctag tcattatgaa 4201 aagttgaagg gtagtccaga agataacgaa caaaaacaat tgtttgtgga gcagcataag 4261 cattatttag atgagattat tgagcaaatc agtgaatttt ctaagcgtgt tattttagca 4321 gatgccaatt tagataaagt tcttagtgca tataacaaac atagagacaa accaatacgt 4381 gaacaagcag aaaatattat tcatttattt acgttgacga atcttggagc tcccgctgct 4441 tttaaatatt ttgatacaac aattgatcgt aaacgatata cgtctacaaa agaagtttta 4501 gatgccactc ttatccatca atccatcact ggtctttatg aaacacgcat tgatttgagt 4561 cagctaggag gtgactgaag tatattttag atgaagatta tttcttaata actaaaaata 4621 tggtataata ctcttaataa atgcagtaat acaggggctt ttcaagactg aagtctagct 4681 gagacaaata gtgcgattac gaaatttttt agacaaaaat agtctacgag gttttagagc 4741 tatgctgttt tgaatggtcc caaaactgag accatcacaa tcggtttgcg attggtctcg 4801 ttttagagct atgctgtttt gaatggtccc aaaacttcag cacactgaga cttgttgagt 4861 tccatgtttt agagctatgc tgttttgaat ggactccatt caacattgcc gatgataact 4921 tgagaaagag ggttaatacc agcagtcgga taccttccta ttctttctgt taaagcgttt 4981 tcatgttata ataggcaaaa gaagagtagt g //