LOCUS       BBa_K1475000            1643 bp    DNA     linear       23-Nov-2024
DEFINITION  Protein containing all amino acids
ACCESSION   BBa_K1475000
VERSION     BBa_K1475000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    61..63
                     /label=ATG
     misc_feature    1501..1506
                     /label=ATTATT
     misc_feature    1434..1500
                     /label=3xFLAG
     CDS             61..1506
                     /label=BBa_K1475001
     stem_loop       1526..1569
                     /label=stem_loop
     misc_feature    1515..1594
                     /label=BBa_B0010
     terminator      1515..1594
                     /label=BBa_B0010
     stem_loop       1610..1629
                     /label=T7 TE
     misc_feature    1636..1636
                     /label=stop
     polyA_site      1630..1643
                     /label=polya
     misc_feature    1603..1643
                     /label=BBa_B0012
     terminator      1603..1643
                     /label=BBa_B0012
     terminator      1515..1643
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 atgttcgagg caaacggcca agaactgccc cgtggcgagg tgcatgagcg cgcgggggcg
      121 gaggctaaac tcaagtcaga tcagaccaat gaccgcagca accagaactc tttttctgat
      181 gagcagcatt atccgcgtaa ggaggtgacc gcgcagaacc cgggcgtggg tccgctgggc
      241 gagatttgga ctgagcgcaa tagtattcgt gtgaagcagc aagaggaatc ccaggcggaa
      301 gattccaacc aggctggccc cgaagaacaa acgggcgatc gtctgatgtg cgtactgggc
      361 attgataacg acttgaatgt ggatcgtcgt cccaacccgc agcctcagtc ggcgcgctac
      421 ccgtattcac cactgcgtgc tcgtcaaagc gcagcagctc gtgaagattt aggcgcagtc
      481 aatcaacttg aactgctcat ggtgcagcgt aaacgcagct ctaacgctgg cgaagtgctt
      541 cgtccggagc gtagctcgaa ctccggacgt gctcaggtcc gtgacaacga accgcgtgtg
      601 caatctgcac aatccgatga tattgatctc cccgacgagg aagacggccc gtcttctctg
      661 gcccaactgt ccgaaaaatg gccatcacgt ccgaaccgtc gcatgcgtgt tcaaggcatg
      721 cgtcagcgcg tgccggaggg tgtgcagcag ccgggctctg aaagctcgga aaaccaggaa
      781 cttaatcaaa aagataaatg gaatgtctcg ttctccgatg gtgataaccg tgctcgtaat
      841 tcgaccggca atcgtcccat ttacaagaac gacgcgttac tggcggagga tatgacggag
      901 tcccagccaa atgtgggccc atctgaggat ctgacggaag taggtggatt ccagctggaa
      961 ccgcgcgcac tgcgcagctc taattattcc ccggaaatgg ctttaacccc gatttgtcag
     1021 aagcgtccta gtgaaaatat taaagaaaag gattcagagc gtaaaagcct gattgaagcc
     1081 catccacgcg gcaccaactc agtttatcaa ttcggtggtg ccaacgaaag ccgcgagcca
     1141 gaaaaccatc gcttaattaa attacaggaa aatcgcggca cgctgcttaa cccggaccat
     1201 cgctatgcct caaaaggttc tgggagtggc gtgtcccgcg ccaacgacga catcgccgac
     1261 atccaggata acccgcgccc gtatcgcctt ggcaacgcat cgtttcccca gaaccttggt
     1321 ccggccaacg gctcaccagc tatttcaatc acccgtggtg gcggcgccag tcgtgatccg
     1381 atcccattct gtgacaaaga agatcaggac cataagtttg ctcaacagaa acgtgactat
     1441 aaagatcatg atggtgatta caaagaccat gatattgatt ataaggatga tgatgacaaa
     1501 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1561 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1621 gggtgggcct ttctgcgttt ata
//