BBa_K1475000 1 BBa_K1475000 Protein containing all amino acids 2014-08-03T11:00:00Z 2015-05-08T01:10:39Z c. elegans jhijbdjkgfn gjgbjiobgofj ngog false false _1854_ 0 20922 9 It's complicated false codon optimized false Camilla Kongerslev Jakobsen component2394195 1 BBa_R0040 component2394207 1 BBa_B0015 component2394200 1 BBa_K1475001 annotation2394195 1 BBa_R0040 range2394195 1 1 54 annotation2394200 1 BBa_K1475001 range2394200 1 61 1506 annotation2394207 1 BBa_B0015 range2394207 1 1515 1643 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 annotation1986787 1 -10 range1986787 1 43 48 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_K1475001 1 OneProt OneProt coding region 2014-08-04T11:00:00Z 2015-05-08T01:10:39Z The sequence is based on WHO guidelines for amounts of amino acids needed in a normal human. j false false _1854_ 0 20911 9 It's complicated true The WHO advised amino acid composition was written down and the sequence was then shuffled using (LINK). A 3*FLAG-tag was then added by remiving the respective codons and adding them to the end of the protein to keep the right composition of amino acids. false Camilla Kongerslev Jakobsen annotation2419615 1 ATTATT range2419615 1 1441 1446 annotation2419616 1 3xFLAG range2419616 1 1374 1440 annotation2419614 1 ATG range2419614 1 1 3 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1475001_sequence 1 atgttcgaggcaaacggccaagaactgccccgtggcgaggtgcatgagcgcgcgggggcggaggctaaactcaagtcagatcagaccaatgaccgcagcaaccagaactctttttctgatgagcagcattatccgcgtaaggaggtgaccgcgcagaacccgggcgtgggtccgctgggcgagatttggactgagcgcaatagtattcgtgtgaagcagcaagaggaatcccaggcggaagattccaaccaggctggccccgaagaacaaacgggcgatcgtctgatgtgcgtactgggcattgataacgacttgaatgtggatcgtcgtcccaacccgcagcctcagtcggcgcgctacccgtattcaccactgcgtgctcgtcaaagcgcagcagctcgtgaagatttaggcgcagtcaatcaacttgaactgctcatggtgcagcgtaaacgcagctctaacgctggcgaagtgcttcgtccggagcgtagctcgaactccggacgtgctcaggtccgtgacaacgaaccgcgtgtgcaatctgcacaatccgatgatattgatctccccgacgaggaagacggcccgtcttctctggcccaactgtccgaaaaatggccatcacgtccgaaccgtcgcatgcgtgttcaaggcatgcgtcagcgcgtgccggagggtgtgcagcagccgggctctgaaagctcggaaaaccaggaacttaatcaaaaagataaatggaatgtctcgttctccgatggtgataaccgtgctcgtaattcgaccggcaatcgtcccatttacaagaacgacgcgttactggcggaggatatgacggagtcccagccaaatgtgggcccatctgaggatctgacggaagtaggtggattccagctggaaccgcgcgcactgcgcagctctaattattccccggaaatggctttaaccccgatttgtcagaagcgtcctagtgaaaatattaaagaaaaggattcagagcgtaaaagcctgattgaagcccatccacgcggcaccaactcagtttatcaattcggtggtgccaacgaaagccgcgagccagaaaaccatcgcttaattaaattacaggaaaatcgcggcacgctgcttaacccggaccatcgctatgcctcaaaaggttctgggagtggcgtgtcccgcgccaacgacgacatcgccgacatccaggataacccgcgcccgtatcgccttggcaacgcatcgtttccccagaaccttggtccggccaacggctcaccagctatttcaatcacccgtggtggcggcgccagtcgtgatccgatcccattctgtgacaaagaagatcaggaccataagtttgctcaacagaaacgtgactataaagatcatgatggtgattacaaagaccatgatattgattataaggatgatgatgacaaataataa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K1475000_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgttcgaggcaaacggccaagaactgccccgtggcgaggtgcatgagcgcgcgggggcggaggctaaactcaagtcagatcagaccaatgaccgcagcaaccagaactctttttctgatgagcagcattatccgcgtaaggaggtgaccgcgcagaacccgggcgtgggtccgctgggcgagatttggactgagcgcaatagtattcgtgtgaagcagcaagaggaatcccaggcggaagattccaaccaggctggccccgaagaacaaacgggcgatcgtctgatgtgcgtactgggcattgataacgacttgaatgtggatcgtcgtcccaacccgcagcctcagtcggcgcgctacccgtattcaccactgcgtgctcgtcaaagcgcagcagctcgtgaagatttaggcgcagtcaatcaacttgaactgctcatggtgcagcgtaaacgcagctctaacgctggcgaagtgcttcgtccggagcgtagctcgaactccggacgtgctcaggtccgtgacaacgaaccgcgtgtgcaatctgcacaatccgatgatattgatctccccgacgaggaagacggcccgtcttctctggcccaactgtccgaaaaatggccatcacgtccgaaccgtcgcatgcgtgttcaaggcatgcgtcagcgcgtgccggagggtgtgcagcagccgggctctgaaagctcggaaaaccaggaacttaatcaaaaagataaatggaatgtctcgttctccgatggtgataaccgtgctcgtaattcgaccggcaatcgtcccatttacaagaacgacgcgttactggcggaggatatgacggagtcccagccaaatgtgggcccatctgaggatctgacggaagtaggtggattccagctggaaccgcgcgcactgcgcagctctaattattccccggaaatggctttaaccccgatttgtcagaagcgtcctagtgaaaatattaaagaaaaggattcagagcgtaaaagcctgattgaagcccatccacgcggcaccaactcagtttatcaattcggtggtgccaacgaaagccgcgagccagaaaaccatcgcttaattaaattacaggaaaatcgcggcacgctgcttaacccggaccatcgctatgcctcaaaaggttctgggagtggcgtgtcccgcgccaacgacgacatcgccgacatccaggataacccgcgcccgtatcgccttggcaacgcatcgtttccccagaaccttggtccggccaacggctcaccagctatttcaatcacccgtggtggcggcgccagtcgtgatccgatcccattctgtgacaaagaagatcaggaccataagtttgctcaacagaaacgtgactataaagatcatgatggtgattacaaagaccatgatattgattataaggatgatgatgacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z