##gff-version 3
##sequence-region BBa_K1475000 1 1643
BBa_K1475000	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1475000	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1475000	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1475000	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1475000	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1475000	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1475000	.	terminator	1515	1643	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1475000	.	terminator	1515	1594	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1475000	.	engineered_region	1515	1594	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1475000	.	stem_loop	1526	1569	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1475000	.	terminator	1603	1643	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1475000	.	stem_loop	1610	1629	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1475000	.	engineered_region	1603	1643	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1475000	.	polyA_site	1630	1643	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1475000	.	stop_codon	1636	1636	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1475000	.	CDS	61	1506	.	+	0	ID=BBa_K1475001;Name=BBa_K1475001
BBa_K1475000	.	start_codon	61	63	.	+	0	ID=annotation2419614;Name=ATG;Parent=BBa_K1475001
BBa_K1475000	.	stop_codon	1501	1506	.	+	0	ID=annotation2419615;Name=ATTATT;Parent=BBa_K1475001
BBa_K1475000	.	tag	1434	1500	.	+	0	ID=annotation2419616;Name=3xFLAG;Parent=BBa_K1475001
>BBa_K1475000
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgttcgaggcaaacggcc
aagaactgccccgtggcgaggtgcatgagcgcgcgggggcggaggctaaactcaagtcagatcagaccaatgaccgcag
caaccagaactctttttctgatgagcagcattatccgcgtaaggaggtgaccgcgcagaacccgggcgtgggtccgctg
ggcgagatttggactgagcgcaatagtattcgtgtgaagcagcaagaggaatcccaggcggaagattccaaccaggctg
gccccgaagaacaaacgggcgatcgtctgatgtgcgtactgggcattgataacgacttgaatgtggatcgtcgtcccaa
cccgcagcctcagtcggcgcgctacccgtattcaccactgcgtgctcgtcaaagcgcagcagctcgtgaagatttaggc
gcagtcaatcaacttgaactgctcatggtgcagcgtaaacgcagctctaacgctggcgaagtgcttcgtccggagcgta
gctcgaactccggacgtgctcaggtccgtgacaacgaaccgcgtgtgcaatctgcacaatccgatgatattgatctccc
cgacgaggaagacggcccgtcttctctggcccaactgtccgaaaaatggccatcacgtccgaaccgtcgcatgcgtgtt
caaggcatgcgtcagcgcgtgccggagggtgtgcagcagccgggctctgaaagctcggaaaaccaggaacttaatcaaa
aagataaatggaatgtctcgttctccgatggtgataaccgtgctcgtaattcgaccggcaatcgtcccatttacaagaa
cgacgcgttactggcggaggatatgacggagtcccagccaaatgtgggcccatctgaggatctgacggaagtaggtgga
ttccagctggaaccgcgcgcactgcgcagctctaattattccccggaaatggctttaaccccgatttgtcagaagcgtc
ctagtgaaaatattaaagaaaaggattcagagcgtaaaagcctgattgaagcccatccacgcggcaccaactcagttta
tcaattcggtggtgccaacgaaagccgcgagccagaaaaccatcgcttaattaaattacaggaaaatcgcggcacgctg
cttaacccggaccatcgctatgcctcaaaaggttctgggagtggcgtgtcccgcgccaacgacgacatcgccgacatcc
aggataacccgcgcccgtatcgccttggcaacgcatcgtttccccagaaccttggtccggccaacggctcaccagctat
ttcaatcacccgtggtggcggcgccagtcgtgatccgatcccattctgtgacaaagaagatcaggaccataagtttgct
caacagaaacgtgactataaagatcatgatggtgattacaaagaccatgatattgattataaggatgatgatgacaaat
aataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata