LOCUS       BBa_K1475004             717 bp    DNA     linear       22-Nov-2024
DEFINITION  Cons. promoter, RBS, TetR and a double terminator
ACCESSION   BBa_K1475004
VERSION     BBa_K1475004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     RBS             36..50
                     /label=RBS-1\Strong
     RBS             43..47
                     /label=RBS
     misc_feature    36..50
                     /label=BBa_B0030
     RBS             36..50
                     /label=BBa_B0030
     misc_feature    51..56
                     /label=BBa_K1088022
     misc_feature    57..59
                     /label=ATG
     misc_feature    678..683
                     /label=TAATAA
     CDS             57..683
                     /label=BBa_K1475003
     misc_feature    684..717
                     /label=B1002
     stem_loop       693..708
                     /label=stem loop
     polyA_site      708..714
                     /label=Poly A tail
     polyA_site      687..692
                     /label=Poly A tail
     terminator      684..717
                     /label=BBa_B1002
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagcattaa agaggagaaa tactagatgt
       61 ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa
      121 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt
      181 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata
      241 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata
      301 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag
      361 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc
      421 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt
      481 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta
      541 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg
      601 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta
      661 aatgtgaaag tgggtcctaa taacgcaaaa aaccccgctt cggcggggtt ttttcgc
//