BBa_B1002
1
BBa_B1002
Terminator (artificial, small, %T~=85%)
2006-08-29T11:00:00Z
2015-08-31T04:07:21Z
antiquity
Artifical terminator, estimated %T~=85
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
true
Haiyao Huang
annotation1898412
1
B1002
range1898412
1
1
34
annotation1898414
1
Poly A tail
range1898414
1
25
31
annotation1898415
1
Poly A tail
range1898415
1
4
9
annotation1898413
1
stem loop
range1898413
1
10
25
BBa_K1475003
1
BBa_K1475003
tetracyclin repressor from transposon Tn10
2014-10-04T11:00:00Z
2015-05-08T01:10:39Z
This part was constructed from the tetracycline repressor from transposon Tn10 (+LVA) (Part:BBa_C0040) using primers to remove the LVA protein digestion tag. That is THIS PART DOES NOT CONTAIN THE LVA PROTEIN DIGESTION TAG.
This part contains the coding sequence of the TetR protein. TetR binds to the pTet promoter and represses transcription. aTc (anhydrotetracycline) bind to the TetR protein and inhibits the function of TetR.
false
false
_1854_
0
20911
9
It's complicated
false
Skrive hvilke primere der er brugt.
false
Daniel Weltz Pedersen
annotation2420139
1
ATG
range2420139
1
1
3
annotation2420140
1
TAATAA
range2420140
1
622
627
BBa_K1475004
1
BBa_K1475004
Cons. promoter, RBS, TetR and a double terminator
2014-10-04T11:00:00Z
2015-05-08T01:10:39Z
-
This device will produce the TetR protein (Part:BBa_K1475003) using a constitutive promoter (Part:BBa_J23106) and RBS (Part:BBa_B0030) followed by a small artificial terminator (Part:BBa_B1002).
false
false
_1854_
0
20911
9
It's complicated
false
-
false
Daniel Weltz Pedersen
component2423696
1
BBa_B0030
component2423701
1
BBa_K1475003
component2423706
1
BBa_B1002
component2423698
1
BBa_K1088022
component2423694
1
BBa_J23106
annotation2423696
1
BBa_B0030
range2423696
1
36
50
annotation2423706
1
BBa_B1002
range2423706
1
684
717
annotation2423694
1
BBa_J23106
range2423694
1
1
35
annotation2423701
1
BBa_K1475003
range2423701
1
57
683
annotation2423698
1
BBa_K1088022
range2423698
1
51
56
BBa_K1088022
1
BBa_K1088022
TACTAG
2013-09-16T11:00:00Z
2015-05-08T01:09:06Z
TACTAG
Short DNA piece (TACTAG)
false
false
_1398_
0
17057
9
Not in stock
false
6 random nucleotides
false
Patrick Rosendahl Andreassen
BBa_J23106
1
BBa_J23106
constitutive promoter family member
2006-08-13T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_K1475003_sequence
1
atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcctaataa
BBa_K1475004_sequence
1
tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcctaataacgcaaaaaaccccgcttcggcggggttttttcgc
BBa_B1002_sequence
1
cgcaaaaaaccccgcttcggcggggttttttcgc
BBa_K1088022_sequence
1
tactag
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_J23106_sequence
1
tttacggctagctcagtcctaggtatagtgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z