BBa_K1475005
type: ComponentDefinition
persistent identity: BBa K1475005
display id: BBa_K1475005
version: 1
was generated by: iGEM to SBOL conversion
description: GFP controlled by TetR and pTet.
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:39Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: This composite part has GFP controlled by a TetR pTet expression system. The Tet repressor protein binds to the Tet promoter, the tet repressor protein is inhibited by aTc hereby inducing the expression of GFP.
discontinued: false
dominant: false
experience: experience/None
group u list: _1854_
m user id: 0
owner id: 20911
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: -
star: false
creator: Daniel Weltz Pedersen
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1475004
component: BBa_E0840
sequence annotation: BBa_K1475004
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K1475005_sequence/1

BBa_K1475004
type: Component
persistent identity: component2421873
display id: component2421873
version: 1
top level: BBa_K1475005
definition: BBa_K1475004
access: public

BBa_E0840
type: Component
persistent identity: component2421884
display id: component2421884
version: 1
top level: BBa_K1475005
definition: GFP genera
access: public

BBa_K1475004
type: SequenceAnnotation
persistent identity: annotation2421873
display id: annotation2421873
version: 1
top level: BBa_K1475005
location: https://synbiohub.org/public/igem/BBa_K1475005/annotation2421873/range2421873/1
component: BBa_K1475004

https://synbiohub.org/public/igem/BBa_K1475005/annotation2421873/range2421873/1
type: Range
persistent identity: range2421873
display id: range2421873
version: 1
top level: BBa_K1475005
direction: direction/reverse
start: 1
end: 717
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2421884
display id: annotation2421884
version: 1
top level: BBa_K1475005
location: https://synbiohub.org/public/igem/BBa_K1475005/annotation2421884/range2421884/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1475005/annotation2421884/range2421884/1
type: Range
persistent identity: range2421884
display id: range2421884
version: 1
top level: BBa_K1475005
direction: direction/reverse
start: 726
end: 1603
orientation: inline

GFP genera
type: ComponentDefinition
persistent identity: BBa E0840
display id: BBa_E0840
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0030.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0030
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0840_sequence/1

BBa_B0030
type: Component
persistent identity: component1249239
display id: component1249239
version: 1
top level: GFP genera
definition: BBa_B0030
access: public

BBa_B0010
type: Component
persistent identity: component1249247
display id: component1249247
version: 1
top level: GFP genera
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249242
display id: component1249242
version: 1
top level: GFP genera
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249257
display id: component1249257
version: 1
top level: GFP genera
definition: BBa_B0012
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1249239
display id: annotation1249239
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
type: Range
persistent identity: range1249239
display id: range1249239
version: 1
top level: GFP genera
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249242
display id: annotation1249242
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
type: Range
persistent identity: range1249242
display id: range1249242
version: 1
top level: GFP genera
direction: direction/forward
start: 22
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249247
display id: annotation1249247
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
type: Range
persistent identity: range1249247
display id: range1249247
version: 1
top level: GFP genera
direction: direction/forward
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249257
display id: annotation1249257
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
type: Range
persistent identity: range1249257
display id: range1249257
version: 1
top level: GFP genera
direction: direction/forward
start: 838
end: 878
orientation: inline

BBa_K1475004
type: ComponentDefinition
persistent identity: BBa K1475004
display id: BBa_K1475004
version: 1
was generated by: iGEM to SBOL conversion
description: Cons. promoter, RBS, TetR and a double terminator
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:39Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: This device will produce the TetR protein (Part:BBa_K1475003) using a constitutive promoter (Part:BBa_J23106) and RBS (Part:BBa_B0030) followed by a small artificial terminator (Part:BBa_B1002).
discontinued: false
dominant: false
group u list: _1854_
m user id: 0
owner id: 20911
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Daniel Weltz Pedersen
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_J23106
component: BBa_K1088022
component: BBa_B0030
component: BBa_B1002
component: BBa_K1475003
sequence annotation: BBa_J23106
sequence annotation: BBa_B0030
sequence annotation: BBa_K1088022
sequence annotation: BBa_K1475003
sequence annotation: BBa_B1002
sequence: https://synbiohub.org/public/igem/BBa_K1475004_sequence/1

BBa_J23106
type: Component
persistent identity: component2423694
display id: component2423694
version: 1
top level: BBa_K1475004
definition: BBa_J23106
access: public

BBa_K1088022
type: Component
persistent identity: component2423698
display id: component2423698
version: 1
top level: BBa_K1475004
definition: BBa_K1088022
access: public

BBa_B0030
type: Component
persistent identity: component2423696
display id: component2423696
version: 1
top level: BBa_K1475004
definition: BBa_B0030
access: public

BBa_B1002
type: Component
persistent identity: component2423706
display id: component2423706
version: 1
top level: BBa_K1475004
definition: BBa_B1002
access: public

BBa_K1475003
type: Component
persistent identity: component2423701
display id: component2423701
version: 1
top level: BBa_K1475004
definition: BBa_K1475003
access: public

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2423694
display id: annotation2423694
version: 1
top level: BBa_K1475004
location: https://synbiohub.org/public/igem/BBa_K1475004/annotation2423694/range2423694/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1475004/annotation2423694/range2423694/1
type: Range
persistent identity: range2423694
display id: range2423694
version: 1
top level: BBa_K1475004
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2423696
display id: annotation2423696
version: 1
top level: BBa_K1475004
location: https://synbiohub.org/public/igem/BBa_K1475004/annotation2423696/range2423696/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1475004/annotation2423696/range2423696/1
type: Range
persistent identity: range2423696
display id: range2423696
version: 1
top level: BBa_K1475004
direction: direction/forward
start: 36
end: 50
orientation: inline

BBa_K1088022
type: SequenceAnnotation
persistent identity: annotation2423698
display id: annotation2423698
version: 1
top level: BBa_K1475004
location: https://synbiohub.org/public/igem/BBa_K1475004/annotation2423698/range2423698/1
component: BBa_K1088022

https://synbiohub.org/public/igem/BBa_K1475004/annotation2423698/range2423698/1
type: Range
persistent identity: range2423698
display id: range2423698
version: 1
top level: BBa_K1475004
direction: direction/reverse
start: 51
end: 56
orientation: inline

BBa_K1475003
type: SequenceAnnotation
persistent identity: annotation2423701
display id: annotation2423701
version: 1
top level: BBa_K1475004
location: https://synbiohub.org/public/igem/BBa_K1475004/annotation2423701/range2423701/1
component: BBa_K1475003

https://synbiohub.org/public/igem/BBa_K1475004/annotation2423701/range2423701/1
type: Range
persistent identity: range2423701
display id: range2423701
version: 1
top level: BBa_K1475004
direction: direction/reverse
start: 57
end: 683
orientation: inline

BBa_B1002
type: SequenceAnnotation
persistent identity: annotation2423706
display id: annotation2423706
version: 1
top level: BBa_K1475004
location: https://synbiohub.org/public/igem/BBa_K1475004/annotation2423706/range2423706/1
component: BBa_B1002

https://synbiohub.org/public/igem/BBa_K1475004/annotation2423706/range2423706/1
type: Range
persistent identity: range2423706
display id: range2423706
version: 1
top level: BBa_K1475004
direction: direction/reverse
start: 684
end: 717
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B1002
type: ComponentDefinition
persistent identity: BBa B1002
display id: BBa_B1002
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, small, %T~=85%)
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: antiquity
owned by: james
owned by: myers
mutable description: Artifical terminator, estimated %T~=85
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1002
sequence annotation: Poly A tail
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B1002_sequence/1

B1002
type: SequenceAnnotation
persistent identity: annotation1898412
display id: annotation1898412
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
type: Range
persistent identity: range1898412
display id: range1898412
version: 1
top level: BBa_B1002
direction: direction/forward
start: 1
end: 34
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898415
display id: annotation1898415
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
type: Range
persistent identity: range1898415
display id: range1898415
version: 1
top level: BBa_B1002
direction: direction/reverse
start: 4
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898413
display id: annotation1898413
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
type: Range
persistent identity: range1898413
display id: range1898413
version: 1
top level: BBa_B1002
direction: direction/forward
start: 10
end: 25
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898414
display id: annotation1898414
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
type: Range
persistent identity: range1898414
display id: range1898414
version: 1
top level: BBa_B1002
direction: direction/forward
start: 25
end: 31
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

BBa_K1088022
type: ComponentDefinition
persistent identity: BBa K1088022
display id: BBa_K1088022
version: 1
was generated by: iGEM to SBOL conversion
description: TACTAG
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: TACTAG
owned by: james
owned by: myers
mutable description: Short DNA piece (TACTAG)
discontinued: false
dominant: false
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: 6 random nucleotides
star: false
creator: Patrick Rosendahl Andreassen
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K1088022_sequence/1

BBa_K1475003
type: ComponentDefinition
persistent identity: BBa K1475003
display id: BBa_K1475003
version: 1
was generated by: iGEM to SBOL conversion
description: tetracyclin repressor from transposon Tn10
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:39Z
mutable provenance: This part was constructed from the tetracycline repressor from transposon Tn10 (+LVA) (Part:BBa_C0040) using primers to remove the LVA protein digestion tag. That is THIS PART DOES NOT CONTAIN THE LVA PROTEIN DIGESTION TAG.
owned by: james
owned by: myers
mutable description: This part contains the coding sequence of the TetR protein. TetR binds to the pTet promoter and represses transcription. aTc (anhydrotetracycline) bind to the TetR protein and inhibits the function of TetR.
discontinued: false
dominant: false
group u list: _1854_
m user id: 0
owner id: 20911
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Skrive hvilke primere der er brugt.
star: false
creator: Daniel Weltz Pedersen
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ATG
sequence annotation: TAATAA
sequence: https://synbiohub.org/public/igem/BBa_K1475003_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2420139
display id: annotation2420139
version: 1
top level: BBa_K1475003
location: https://synbiohub.org/public/igem/BBa_K1475003/annotation2420139/range2420139/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1475003/annotation2420139/range2420139/1
type: Range
persistent identity: range2420139
display id: range2420139
version: 1
top level: BBa_K1475003
direction: direction/forward
start: 1
end: 3
orientation: inline

TAATAA
type: SequenceAnnotation
persistent identity: annotation2420140
display id: annotation2420140
version: 1
top level: BBa_K1475003
location: https://synbiohub.org/public/igem/BBa_K1475003/annotation2420140/range2420140/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1475003/annotation2420140/range2420140/1
type: Range
persistent identity: range2420140
display id: range2420140
version: 1
top level: BBa_K1475003
direction: direction/forward
start: 622
end: 627
orientation: inline

https://synbiohub.org/public/igem/BBa_K1475005_sequence/1
type: Sequence
persistent identity: BBa K1475005 sequence
display id: BBa_K1475005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcctaataacgcaaaaaaccccgcttcggcggggttttttcgctactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0840_sequence/1
type: Sequence
persistent identity: BBa E0840 sequence
display id: BBa_E0840_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1475004_sequence/1
type: Sequence
persistent identity: BBa K1475004 sequence
display id: BBa_K1475004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcctaataacgcaaaaaaccccgcttcggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1002_sequence/1
type: Sequence
persistent identity: BBa B1002 sequence
display id: BBa_B1002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcaaaaaaccccgcttcggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23106_sequence/1
type: Sequence
persistent identity: BBa J23106 sequence
display id: BBa_J23106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088022_sequence/1
type: Sequence
persistent identity: BBa K1088022 sequence
display id: BBa_K1088022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1475003_sequence/1
type: Sequence
persistent identity: BBa K1475003 sequence
display id: BBa_K1475003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcctaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z