This composite part has GFP controlled by a TetR pTet expression system. The Tet repressor protein binds to the Tet promoter, the tet repressor protein is inhibited by aTc hereby inducing the expression of GFP.
Cons. promoter, RBS, TetR and a double terminator
This device will produce the TetR protein (Part:BBa_K1475003) using a constitutive promoter (Part:BBa_J23106) and RBS (Part:BBa_B0030) followed by a small artificial terminator (Part:BBa_B1002).
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
RBS.1 (strong) -- modified from R. Weiss
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
GFP (mut3b) [note that this part does not have a barcode]
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
tetracyclin repressor from transposon Tn10
This part was constructed from the tetracycline repressor from transposon Tn10 (+LVA) (Part:BBa_C0040) using primers to remove the LVA protein digestion tag. That is THIS PART DOES NOT CONTAIN THE LVA PROTEIN DIGESTION TAG.
This part contains the coding sequence of the TetR protein. TetR binds to the pTet promoter and represses transcription. aTc (anhydrotetracycline) bind to the TetR protein and inhibits the function of TetR.
Conversion of the iGEM parts registry to SBOL2.1