LOCUS       BBa_K1475006            1698 bp    DNA     linear       25-Nov-2024
DEFINITION  GFP controlled by TetR+LVA and pTet.
ACCESSION   BBa_K1475006
VERSION     BBa_K1475006.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     RBS             36..50
                     /label=RBS-1\Strong
     RBS             43..47
                     /label=RBS
     misc_feature    36..50
                     /label=BBa_B0030
     RBS             36..50
                     /label=BBa_B0030
     misc_feature    1..50
                     /label=BBa_K1475900
     CDS             57..716
                     /label=BBa_K1475901
     misc_feature    717..750
                     /label=B1002
     stem_loop       726..741
                     /label=stem loop
     polyA_site      741..747
                     /label=Poly A tail
     polyA_site      720..725
                     /label=Poly A tail
     terminator      717..750
                     /label=BBa_B1002
     misc_feature    57..750
                     /label=BBa_K1475903
     misc_feature    759..777
                     /label=TetR 1
     misc_feature    759..812
                     /label=BBa_R0040
     promoter        778..783
                     /label=-35
     misc_feature    784..802
                     /label=TetR 2
     promoter        801..806
                     /label=-10
     promoter        759..812
                     /label=BBa_R0040
     RBS             821..835
                     /label=RBS-1\Strong
     RBS             828..832
                     /label=RBS
     misc_feature    821..835
                     /label=BBa_B0030
     RBS             821..835
                     /label=BBa_B0030
     misc_feature    759..835
                     /label=BBa_K1475904
     CDS             842..1561
                     /label=GFP protein
     CDS             842..1561
                     /label=BBa_E0040
     stem_loop       1581..1624
                     /label=stem_loop
     misc_feature    1570..1649
                     /label=BBa_B0010
     terminator      1570..1649
                     /label=BBa_B0010
     stem_loop       1665..1684
                     /label=T7 TE
     misc_feature    1691..1691
                     /label=stop
     polyA_site      1685..1698
                     /label=polya
     misc_feature    1658..1698
                     /label=BBa_B0012
     terminator      1658..1698
                     /label=BBa_B0012
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagcattaa agaggagaaa tactagatgt
       61 ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa
      121 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt
      181 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata
      241 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata
      301 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag
      361 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc
      421 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt
      481 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta
      541 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg
      601 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta
      661 aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacgca
      721 aaaaaccccg cttcggcggg gttttttcgc tactagagtc cctatcagtg atagagattg
      781 acatccctat cagtgataga gatactgagc actactagag attaaagagg agaaatacta
      841 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      901 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      961 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
     1021 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
     1081 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     1141 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
     1201 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
     1261 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
     1321 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
     1381 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     1441 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     1501 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
     1561 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1621 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1681 ggcctttctg cgtttata
//