##gff-version 3
##sequence-region BBa_K1475006 1 1698
BBa_K1475006	.	CDS	842	1561	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K1475006	.	CDS	842	1561	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1475006	.	engineered_region	57	750	.	+	0	ID=BBa_K1475903;Name=BBa_K1475903
BBa_K1475006	.	terminator	717	750	.	+	0	ID=BBa_B1002;Name=BBa_B1002;Parent=BBa_K1475903
BBa_K1475006	.	engineered_region	717	750	.	+	0	ID=annotation1898412;Name=B1002;Parent=BBa_B1002
BBa_K1475006	.	polyA_site	720	725	.	+	0	ID=annotation1898415;Name=Poly A tail;Parent=BBa_B1002
BBa_K1475006	.	polyA_site	741	747	.	+	0	ID=annotation1898414;Name=Poly A tail;Parent=BBa_B1002
BBa_K1475006	.	stem_loop	726	741	.	+	0	ID=annotation1898413;Name=stem loop;Parent=BBa_B1002
BBa_K1475006	.	CDS	57	716	.	+	0	ID=BBa_K1475901;Name=BBa_K1475901;Parent=BBa_K1475903
BBa_K1475006	.	engineered_region	759	835	.	+	0	ID=BBa_K1475904;Name=BBa_K1475904
BBa_K1475006	.	promoter	759	812	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1475904
BBa_K1475006	.	promoter	778	783	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1475006	.	non_covalent_binding_site	784	802	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1475006	.	engineered_region	759	812	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1475006	.	promoter	801	806	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1475006	.	non_covalent_binding_site	759	777	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1475006	.	ribosome_entry_site	821	835	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1475904
BBa_K1475006	.	engineered_region	821	835	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1475006	.	ribosome_entry_site	828	832	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1475006	.	ribosome_entry_site	821	835	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1475006	.	engineered_region	1	50	.	+	0	ID=BBa_K1475900;Name=BBa_K1475900
BBa_K1475006	.	promoter	1	35	.	+	0	ID=BBa_J23106;Name=BBa_J23106;Parent=BBa_K1475900
BBa_K1475006	.	ribosome_entry_site	36	50	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1475900
BBa_K1475006	.	engineered_region	36	50	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1475006	.	ribosome_entry_site	43	47	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1475006	.	ribosome_entry_site	36	50	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1475006	.	terminator	1658	1698	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1475006	.	engineered_region	1658	1698	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1475006	.	stop_codon	1691	1691	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1475006	.	stem_loop	1665	1684	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1475006	.	polyA_site	1685	1698	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1475006	.	terminator	1570	1649	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1475006	.	engineered_region	1570	1649	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1475006	.	stem_loop	1581	1624	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K1475006
tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatgtccagattagataaaagtaa
agtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagcta
ggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttag
ataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttag
atgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctc
gaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcatt
ttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtat
gccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattg
atcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagctt
aataacgcaaaaaaccccgcttcggcggggttttttcgctactagagtccctatcagtgatagagattgacatccctat
cagtgatagagatactgagcactactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactg
gagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtga
tgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcact
actttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgc
ccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttga
aggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattg
gaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatta
gacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcct
tttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcctt
cttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttata