BBa_K1475006
type: ComponentDefinition
persistent identity: BBa K1475006
display id: BBa_K1475006
version: 1
was generated by: iGEM to SBOL conversion
description: GFP controlled by TetR+LVA and pTet.
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:39Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: This composite part has GFP controlled by a TetR+LVA pTet expression system. The Tet repressor protein binds to the Tet promoter, the tet repressor protein is inhibited by aTc hereby inducing the expression of GFP.
discontinued: false
dominant: false
experience: experience/None
group u list: _1854_
m user id: 0
owner id: 20911
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: -
star: false
creator: Daniel Weltz Pedersen
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0010
component: BBa_E0040
component: BBa_K1475904
component: BBa_K1475903
component: BBa_B0012
component: BBa_K1475900
sequence annotation: BBa_K1475900
sequence annotation: BBa_K1475903
sequence annotation: BBa_K1475904
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1475006_sequence/1

BBa_B0010
type: Component
persistent identity: component2424681
display id: component2424681
version: 1
top level: BBa_K1475006
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component2424680
display id: component2424680
version: 1
top level: BBa_K1475006
definition: GFP
access: public

BBa_K1475904
type: Component
persistent identity: component2424678
display id: component2424678
version: 1
top level: BBa_K1475006
definition: BBa_K1475904
access: public

BBa_K1475903
type: Component
persistent identity: component2424669
display id: component2424669
version: 1
top level: BBa_K1475006
definition: BBa_K1475903
access: public

BBa_B0012
type: Component
persistent identity: component2424683
display id: component2424683
version: 1
top level: BBa_K1475006
definition: BBa_B0012
access: public

BBa_K1475900
type: Component
persistent identity: component2424662
display id: component2424662
version: 1
top level: BBa_K1475006
definition: BBa_K1475900
access: public

BBa_K1475900
type: SequenceAnnotation
persistent identity: annotation2424662
display id: annotation2424662
version: 1
top level: BBa_K1475006
location: https://synbiohub.org/public/igem/BBa_K1475006/annotation2424662/range2424662/1
component: BBa_K1475900

https://synbiohub.org/public/igem/BBa_K1475006/annotation2424662/range2424662/1
type: Range
persistent identity: range2424662
display id: range2424662
version: 1
top level: BBa_K1475006
direction: direction/reverse
start: 1
end: 50
orientation: inline

BBa_K1475903
type: SequenceAnnotation
persistent identity: annotation2424669
display id: annotation2424669
version: 1
top level: BBa_K1475006
location: https://synbiohub.org/public/igem/BBa_K1475006/annotation2424669/range2424669/1
component: BBa_K1475903

https://synbiohub.org/public/igem/BBa_K1475006/annotation2424669/range2424669/1
type: Range
persistent identity: range2424669
display id: range2424669
version: 1
top level: BBa_K1475006
direction: direction/reverse
start: 57
end: 750
orientation: inline

BBa_K1475904
type: SequenceAnnotation
persistent identity: annotation2424678
display id: annotation2424678
version: 1
top level: BBa_K1475006
location: https://synbiohub.org/public/igem/BBa_K1475006/annotation2424678/range2424678/1
component: BBa_K1475904

https://synbiohub.org/public/igem/BBa_K1475006/annotation2424678/range2424678/1
type: Range
persistent identity: range2424678
display id: range2424678
version: 1
top level: BBa_K1475006
direction: direction/reverse
start: 759
end: 835
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2424680
display id: annotation2424680
version: 1
top level: BBa_K1475006
location: https://synbiohub.org/public/igem/BBa_K1475006/annotation2424680/range2424680/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1475006/annotation2424680/range2424680/1
type: Range
persistent identity: range2424680
display id: range2424680
version: 1
top level: BBa_K1475006
direction: direction/reverse
start: 842
end: 1561
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2424681
display id: annotation2424681
version: 1
top level: BBa_K1475006
location: https://synbiohub.org/public/igem/BBa_K1475006/annotation2424681/range2424681/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1475006/annotation2424681/range2424681/1
type: Range
persistent identity: range2424681
display id: range2424681
version: 1
top level: BBa_K1475006
direction: direction/forward
start: 1570
end: 1649
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2424683
display id: annotation2424683
version: 1
top level: BBa_K1475006
location: https://synbiohub.org/public/igem/BBa_K1475006/annotation2424683/range2424683/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1475006/annotation2424683/range2424683/1
type: Range
persistent identity: range2424683
display id: range2424683
version: 1
top level: BBa_K1475006
direction: direction/forward
start: 1658
end: 1698
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K1475900
type: ComponentDefinition
persistent identity: BBa K1475900
display id: BBa_K1475900
version: 1
was generated by: iGEM to SBOL conversion
description: Pcon+RBS
created: 2014-10-15T11:00:00Z
modified: 2015-05-08T01:10:40Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
group u list: _1854_
m user id: 0
owner id: 20911
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Daniel Weltz Pedersen
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23106
component: BBa_B0030
sequence annotation: BBa_J23106
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K1475900_sequence/1

BBa_J23106
type: Component
persistent identity: component2422021
display id: component2422021
version: 1
top level: BBa_K1475900
definition: BBa_J23106
access: public

BBa_B0030
type: Component
persistent identity: component2422023
display id: component2422023
version: 1
top level: BBa_K1475900
definition: BBa_B0030
access: public

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2422021
display id: annotation2422021
version: 1
top level: BBa_K1475900
location: https://synbiohub.org/public/igem/BBa_K1475900/annotation2422021/range2422021/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1475900/annotation2422021/range2422021/1
type: Range
persistent identity: range2422021
display id: range2422021
version: 1
top level: BBa_K1475900
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2422023
display id: annotation2422023
version: 1
top level: BBa_K1475900
location: https://synbiohub.org/public/igem/BBa_K1475900/annotation2422023/range2422023/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1475900/annotation2422023/range2422023/1
type: Range
persistent identity: range2422023
display id: range2422023
version: 1
top level: BBa_K1475900
direction: direction/forward
start: 36
end: 50
orientation: inline

BBa_K1475903
type: ComponentDefinition
persistent identity: BBa K1475903
display id: BBa_K1475903
version: 1
was generated by: iGEM to SBOL conversion
description: tetR with LVA and terminator
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:40Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: This part contains the tetR coding sequence + an LVA tag. The terminator is a small artificial terminator practical for implementing via PCR.
discontinued: false
dominant: false
group u list: _1854_
m user id: 0
owner id: 20911
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Daniel Weltz Pedersen
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B1002
component: BBa_K1475901
sequence annotation: BBa_K1475901
sequence annotation: BBa_B1002
sequence: https://synbiohub.org/public/igem/BBa_K1475903_sequence/1

BBa_B1002
type: Component
persistent identity: component2423529
display id: component2423529
version: 1
top level: BBa_K1475903
definition: BBa_B1002
access: public

BBa_K1475901
type: Component
persistent identity: component2423524
display id: component2423524
version: 1
top level: BBa_K1475903
definition: BBa_K1475901
access: public

BBa_K1475901
type: SequenceAnnotation
persistent identity: annotation2423524
display id: annotation2423524
version: 1
top level: BBa_K1475903
location: https://synbiohub.org/public/igem/BBa_K1475903/annotation2423524/range2423524/1
component: BBa_K1475901

https://synbiohub.org/public/igem/BBa_K1475903/annotation2423524/range2423524/1
type: Range
persistent identity: range2423524
display id: range2423524
version: 1
top level: BBa_K1475903
direction: direction/reverse
start: 1
end: 660
orientation: inline

BBa_B1002
type: SequenceAnnotation
persistent identity: annotation2423529
display id: annotation2423529
version: 1
top level: BBa_K1475903
location: https://synbiohub.org/public/igem/BBa_K1475903/annotation2423529/range2423529/1
component: BBa_B1002

https://synbiohub.org/public/igem/BBa_K1475903/annotation2423529/range2423529/1
type: Range
persistent identity: range2423529
display id: range2423529
version: 1
top level: BBa_K1475903
direction: direction/reverse
start: 661
end: 694
orientation: inline

BBa_K1475904
type: ComponentDefinition
persistent identity: BBa K1475904
display id: BBa_K1475904
version: 1
was generated by: iGEM to SBOL conversion
description: p(tetR) repressible promoter and RBS
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:40Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: This part contains the p(tetR) repressible promoter and an artificial RBS.
discontinued: false
dominant: false
group u list: _1854_
m user id: 0
owner id: 20911
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Daniel Weltz Pedersen
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0030
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K1475904_sequence/1

BBa_B0030
type: Component
persistent identity: component2424629
display id: component2424629
version: 1
top level: BBa_K1475904
definition: BBa_B0030
access: public

BBa_R0040
type: Component
persistent identity: component2424623
display id: component2424623
version: 1
top level: BBa_K1475904
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2424623
display id: annotation2424623
version: 1
top level: BBa_K1475904
location: https://synbiohub.org/public/igem/BBa_K1475904/annotation2424623/range2424623/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1475904/annotation2424623/range2424623/1
type: Range
persistent identity: range2424623
display id: range2424623
version: 1
top level: BBa_K1475904
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2424629
display id: annotation2424629
version: 1
top level: BBa_K1475904
location: https://synbiohub.org/public/igem/BBa_K1475904/annotation2424629/range2424629/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1475904/annotation2424629/range2424629/1
type: Range
persistent identity: range2424629
display id: range2424629
version: 1
top level: BBa_K1475904
direction: direction/forward
start: 63
end: 77
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B1002
type: ComponentDefinition
persistent identity: BBa B1002
display id: BBa_B1002
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, small, %T~=85%)
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: antiquity
owned by: james
owned by: myers
mutable description: Artifical terminator, estimated %T~=85
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1002
sequence annotation: Poly A tail
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B1002_sequence/1

B1002
type: SequenceAnnotation
persistent identity: annotation1898412
display id: annotation1898412
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
type: Range
persistent identity: range1898412
display id: range1898412
version: 1
top level: BBa_B1002
direction: direction/forward
start: 1
end: 34
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898415
display id: annotation1898415
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
type: Range
persistent identity: range1898415
display id: range1898415
version: 1
top level: BBa_B1002
direction: direction/reverse
start: 4
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898413
display id: annotation1898413
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
type: Range
persistent identity: range1898413
display id: range1898413
version: 1
top level: BBa_B1002
direction: direction/forward
start: 10
end: 25
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898414
display id: annotation1898414
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
type: Range
persistent identity: range1898414
display id: range1898414
version: 1
top level: BBa_B1002
direction: direction/forward
start: 25
end: 31
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

BBa_K1475901
type: ComponentDefinition
persistent identity: BBa K1475901
display id: BBa_K1475901
version: 1
was generated by: iGEM to SBOL conversion
description: TetR without LVA and barcode
created: 2014-10-15T11:00:00Z
modified: 2015-05-08T01:10:40Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: This part is the coding sequence of the TetR repressor.
discontinued: false
dominant: false
group u list: _1854_
m user id: 0
owner id: 20911
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Daniel Weltz Pedersen
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1475901_sequence/1

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_K1475006_sequence/1
type: Sequence
persistent identity: BBa K1475006 sequence
display id: BBa_K1475006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacgcaaaaaaccccgcttcggcggggttttttcgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1475900_sequence/1
type: Sequence
persistent identity: BBa K1475900 sequence
display id: BBa_K1475900_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1475903_sequence/1
type: Sequence
persistent identity: BBa K1475903 sequence
display id: BBa_K1475903_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacgcaaaaaaccccgcttcggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1475904_sequence/1
type: Sequence
persistent identity: BBa K1475904 sequence
display id: BBa_K1475904_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1002_sequence/1
type: Sequence
persistent identity: BBa B1002 sequence
display id: BBa_B1002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcaaaaaaccccgcttcggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23106_sequence/1
type: Sequence
persistent identity: BBa J23106 sequence
display id: BBa_J23106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1475901_sequence/1
type: Sequence
persistent identity: BBa K1475901 sequence
display id: BBa_K1475901_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z