LOCUS       BBa_K1475007            2270 bp    DNA     linear       26-Nov-2024
DEFINITION  GFP controlled by lacI and plac
ACCESSION   BBa_K1475007
VERSION     BBa_K1475007.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     RBS             36..50
                     /label=RBS-1\Strong
     RBS             43..47
                     /label=RBS
     misc_feature    36..50
                     /label=BBa_B0030
     RBS             36..50
                     /label=BBa_B0030
     misc_feature    1..50
                     /label=BBa_K1475900
     CDS             57..1142
                     /label=BBa_I732100
     misc_feature    1143..1176
                     /label=B1002
     stem_loop       1152..1167
                     /label=stem loop
     polyA_site      1167..1173
                     /label=Poly A tail
     polyA_site      1146..1151
                     /label=Poly A tail
     terminator      1143..1176
                     /label=BBa_B1002
     misc_feature    57..1176
                     /label=BBa_K1475902
     misc_feature    1..1176
                     /label=BBa_K1475008
     CDS             1185..1272
                     /label=end of LacI coding region (inactive)
     misc_feature    1185..1384
                     /label=BBa_R0010
     misc_feature    1273..1310
                     /label=CAP binding site
     promoter        1321..1326
                     /label=-35
     promoter        1345..1350
                     /label=-10
     misc_feature    1350..1384
                     /label=LacI binding site
     misc_feature    1357..1357
                     /label=start
     promoter        1185..1384
                     /label=BBa_R0010
     RBS             1393..1407
                     /label=RBS-1\Strong
     RBS             1400..1404
                     /label=RBS
     misc_feature    1393..1407
                     /label=BBa_B0030
     RBS             1393..1407
                     /label=BBa_B0030
     CDS             1414..2133
                     /label=GFP protein
     CDS             1414..2133
                     /label=BBa_E0040
     stem_loop       2153..2196
                     /label=stem_loop
     misc_feature    2142..2221
                     /label=BBa_B0010
     terminator      2142..2221
                     /label=BBa_B0010
     stem_loop       2237..2256
                     /label=T7 TE
     misc_feature    2263..2263
                     /label=stop
     polyA_site      2257..2270
                     /label=polya
     misc_feature    2230..2270
                     /label=BBa_B0012
     terminator      2230..2270
                     /label=BBa_B0012
     misc_feature    1393..2270
                     /label=BBa_E0840
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagcattaa agaggagaaa tactagatga
       61 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
      121 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
      181 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
      241 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
      301 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
      361 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
      421 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
      481 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
      541 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
      601 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
      661 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
      721 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
      781 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
      841 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
      901 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
      961 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
     1021 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
     1081 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtaat
     1141 aacgcaaaaa accccgcttc ggcggggttt tttcgctact agagcaatac gcaaaccgcc
     1201 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1261 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     1321 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     1381 cacatactag agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact
     1441 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
     1501 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
     1561 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
     1621 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
     1681 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
     1741 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
     1801 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
     1861 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
     1921 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
     1981 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
     2041 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
     2101 acacatggca tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg
     2161 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     2221 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//