LOCUS       BBa_K1476000            1374 bp    DNA     linear       12-May-2024
DEFINITION  PilT1 knockout for Synechocystis sp. PCC 6803
ACCESSION   BBa_K1476000
VERSION     BBa_K1476000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..249
                     /label=pilT1 left
     misc_feature    1126..1374
                     /label=pilT1 right
     misc_feature    280..1095
                     /label=KanR
ORIGIN
        1 atggctttgg aatacatgat cgaagacctc atggagcagt tggtggaaat gggcggctcc
       61 gatatgcaca ttcaagcggg ggcaccggtt tatttccggg tgagcggcaa attagaaccg
      121 attaacgagg aagttttaac tccccaggaa agccaaaagt taatcttcag catgctgaac
      181 aattcccaac ggaaagaact agaacaaaat tgggaattgg actgttccta tggcgtgaaa
      241 ggtttagctt gagatgctcg taccgtgatt catgcggcaa tgagccatat tcaacgggaa
      301 acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
      361 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc
      421 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
      481 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
      541 atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc
      601 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
      661 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
      721 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
      781 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca
      841 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
      901 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
      961 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     1021 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     1081 gatgagtttt tctaaaagta gggatagtcc caaacctcac ttaccgtgca ggaaattatg
     1141 gtcattaccc cggcgatcgc caacctaatt cgggaaggca aagcggccca gatttattcc
     1201 gccattcaaa ccggagcaaa actaggtatg cagaccatgg aacagggcct ggccacgttg
     1261 gtggtgtcgg gggtaatttc cctggaagaa ggtttagcta agagtggtaa gccggacgag
     1321 ctacagcgct taatcggtgg catgaccccc caggttgccg ctaaacgtcg ttag
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