BBa_K1476000 1 BBa_K1476000 PilT1 knockout for Synechocystis sp. PCC 6803 2014-10-08T11:00:00Z 2015-05-08T01:10:40Z Organism: Cyanobacteria Genus and Strain: Synechocystis sp. PCC 6803 Source: http://genome.microbedb.jp/cyanobase/Synechocystis/genes/slr0161 Important genotype features: none This part deletes the pilT1 ATPase gene in Synechocystis sp. PCC 6803. PilT1 normally allows for pilus retraction. false false _1856_ 0 21891 9 It's complicated false The part is designed to contained regions homologous to the pilT1 gene. As such we had to ensure that the homologous region was of sufficient length to allow for recombination. The part contains two regions homologous to pilT1: the first region is homologous to the first 200 bases of pilT1, the second region to the last 200 bases of the pilT1 gene. The centre of the part is a kanamycin resistance gene, which becomes inserted in the middle of the chromosomal pilT1 gene during recombination. false Matthew Smith annotation2407302 1 pilT1 right range2407302 1 1126 1374 annotation2407304 1 KanR range2407304 1 280 1095 annotation2407300 1 pilT1 left range2407300 1 1 249 BBa_K1476000_sequence 1 atggctttggaatacatgatcgaagacctcatggagcagttggtggaaatgggcggctccgatatgcacattcaagcgggggcaccggtttatttccgggtgagcggcaaattagaaccgattaacgaggaagttttaactccccaggaaagccaaaagttaatcttcagcatgctgaacaattcccaacggaaagaactagaacaaaattgggaattggactgttcctatggcgtgaaaggtttagcttgagatgctcgtaccgtgattcatgcggcaatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaaaagtagggatagtcccaaacctcacttaccgtgcaggaaattatggtcattaccccggcgatcgccaacctaattcgggaaggcaaagcggcccagatttattccgccattcaaaccggagcaaaactaggtatgcagaccatggaacagggcctggccacgttggtggtgtcgggggtaatttccctggaagaaggtttagctaagagtggtaagccggacgagctacagcgcttaatcggtggcatgaccccccaggttgccgctaaacgtcgttag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z