LOCUS       BBa_K1476001            2551 bp    DNA     linear       13-May-2024
DEFINITION  PilA1 from Synechocystis sp. PCC 6803
ACCESSION   BBa_K1476001
VERSION     BBa_K1476001.1  
FEATURES             Location/Qualifiers
     misc_feature    3..252
                     /label=phaAB left
     CDS             278..1093
                     /label=KanR
     promoter        1110..1669
                     /label=PcPc560
     CDS             1670..2176
                     /label=pilA1
     misc_feature    2177..2276
                     /label=TrbcL-S (terminator)
     misc_feature    2302..2551
                     /label=phaAB right
     misc_feature    1099..1104
                     /label=BioBrick scar site
ORIGIN
        1 agtattgagc ttgttgcaat cttcaccccg gccctgggca cggggtattt tattggtaaa
       61 aatccgatcc tcataggcga tcgccggggg ggatttgttt atttgttgtc aaagcttgaa
      121 atttccaatg ccccgcttgc caaattaatc cccataattg agctggaggc ggagttaatg
      181 gccgcccatc ccaacccatg gcattggaaa acaattatgc gtgatgtttt tattgttgcc
      241 gctaaacgaa cttaatgcgt tcctagccgc tataattatg agccatattc aacgggaaac
      301 gtcttgctcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
      361 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga
      421 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
      481 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
      541 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca
      601 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
      661 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
      721 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
      781 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt
      841 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
      901 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
      961 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     1021 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     1081 tgagtttttc taagttgcta ctagaccaca cctgtagaga agagtccctg aatatcaaaa
     1141 tggtgggata aaaagctcaa aaaggaaagt aggctgtggt tccctaggca acagtcttcc
     1201 ctaccccact ggaaactaaa aaaacgagaa aagttcgcac cgaacatcaa ttgcataatt
     1261 ttagccctaa aacataagct gaacgaaact ggttgtcttc ccttcccaat ccaggacaat
     1321 ctgagaatcc cctgcaacat tacttaacaa aaaagcagga ataaaattaa caagatgtaa
     1381 cagacataag tcccatcacc gttgtataaa gttaactgtg ggattgcaaa agcattcaag
     1441 cctaggcgct gagctgtttg agcatcccgg tggcccttgt cgctgcctcc gtgtttctcc
     1501 ctggatttat ttaggtaata tctctcataa atccccgggt agttaacgaa agttaatgga
     1561 gatcagtaac aataactcta gggtcattac tttggactcc ctcagtttat ccgggggaat
     1621 tgtgtttaag aaaatcccaa ctcataaagt caagtaggag attaattcaa tggctagtaa
     1681 ttttaaattc aaactcctct ctcaactctc caaaaaacgg gcagaaggtg gtttcacctt
     1741 aatcgaactg ctggttgttg taattattat cggtgtattg gctgctattg ctctgcctaa
     1801 cctcttgggt caagttggta aagcacggga gtccgaagct aaatccacta tcggtgcttt
     1861 gaaccgtgcc caacaaggtt attttactga gaagggtacg tttgcaaccg atacggaaac
     1921 gcttgaagtc ccagctcctg atggtaattt ctttagtttt gcagttaaca ctgctgacaa
     1981 cacagaagct atccaagacg caacggcatt gaattgggag gctgacggca cccgctccat
     2041 gtcaggtgga acgttttacg attcaggcac ccgcgccttc agtactgttg tttgtcgggc
     2101 tgaagcaggt agtgaagata ctcccccaac cccaggaggg gctaatgatt gtggtggtgc
     2161 tgaagtaatt aaatagagcc tgatttgtct tgatagctgc tcctgccttt gggcaggggc
     2221 ttttttctgt ctgccattct tgaggatggc ggactctttc ccttttgctc tacgcccata
     2281 cagaagtcta aagcagtgat ctttactttc ccccgtagcc agtagctatg tcaccggcga
     2341 agtattacgg gtaaatgggg cccaccacac ctaacttttg cggtactgtg caagaatcct
     2401 aattttctcc tccctaattc tggggagatt ttttttagcc gtctaaatcc cccgccatca
     2461 ccgtggcgat cgccgttttg gcattgtcct gaaattcccg cacattgact ttgttgagca
     2521 ggaaaatgga gagaattaac cccagggcct g
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