BBa_K1476001 1 BBa_K1476001 PilA1 from Synechocystis sp. PCC 6803 2014-10-08T11:00:00Z 2015-05-08T01:10:40Z Organism: Cyanobacteria Genus and Strain: Synechocystis sp. PCC 6803 Source: http://genome.microbedb.jp/cyanobase/Synechocystis/genes/sll1694 Important genotype features: none This part inserts the pilA1 gene into Synechocystis sp. PCC 6803. The part contains the pilA1 gene and the upstream Pcpc560 super-strong promoter. PilA1 is the main pilus subunit. false false _1856_ 0 21891 9 It's complicated false The part is designed to contained regions homologous to the pilA1 gene. As such we had to ensure that the homologous region was of sufficient length to allow for recombination. The part contains two regions homologous to pilA1: the first region is homologous to the first 200 bases of pilA1, the second region to the last 200 bases of the pilT1 gene. The centre of the part contains the Pcpc560 super-strong promoter, the PilA1 gene and a kanamycin resistance gene, which become inserted in the middle of the chromosomal pilT1 gene during recombination. false Matthew Smith annotation2407327 1 BioBrick scar site range2407327 1 1099 1104 annotation2407321 1 phaAB left range2407321 1 3 252 annotation2407323 1 PcPc560 range2407323 1 1110 1669 annotation2407326 1 phaAB right range2407326 1 2302 2551 annotation2407322 1 KanR range2407322 1 278 1093 annotation2407324 1 pilA1 range2407324 1 1670 2176 annotation2407325 1 TrbcL-S (terminator) range2407325 1 2177 2276 BBa_K1476001_sequence 1 agtattgagcttgttgcaatcttcaccccggccctgggcacggggtattttattggtaaaaatccgatcctcataggcgatcgccgggggggatttgtttatttgttgtcaaagcttgaaatttccaatgccccgcttgccaaattaatccccataattgagctggaggcggagttaatggccgcccatcccaacccatggcattggaaaacaattatgcgtgatgtttttattgttgccgctaaacgaacttaatgcgttcctagccgctataattatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaagttgctactagaccacacctgtagagaagagtccctgaatatcaaaatggtgggataaaaagctcaaaaaggaaagtaggctgtggttccctaggcaacagtcttccctaccccactggaaactaaaaaaacgagaaaagttcgcaccgaacatcaattgcataattttagccctaaaacataagctgaacgaaactggttgtcttcccttcccaatccaggacaatctgagaatcccctgcaacattacttaacaaaaaagcaggaataaaattaacaagatgtaacagacataagtcccatcaccgttgtataaagttaactgtgggattgcaaaagcattcaagcctaggcgctgagctgtttgagcatcccggtggcccttgtcgctgcctccgtgtttctccctggatttatttaggtaatatctctcataaatccccgggtagttaacgaaagttaatggagatcagtaacaataactctagggtcattactttggactccctcagtttatccgggggaattgtgtttaagaaaatcccaactcataaagtcaagtaggagattaattcaatggctagtaattttaaattcaaactcctctctcaactctccaaaaaacgggcagaaggtggtttcaccttaatcgaactgctggttgttgtaattattatcggtgtattggctgctattgctctgcctaacctcttgggtcaagttggtaaagcacgggagtccgaagctaaatccactatcggtgctttgaaccgtgcccaacaaggttattttactgagaagggtacgtttgcaaccgatacggaaacgcttgaagtcccagctcctgatggtaatttctttagttttgcagttaacactgctgacaacacagaagctatccaagacgcaacggcattgaattgggaggctgacggcacccgctccatgtcaggtggaacgttttacgattcaggcacccgcgccttcagtactgttgtttgtcgggctgaagcaggtagtgaagatactcccccaaccccaggaggggctaatgattgtggtggtgctgaagtaattaaatagagcctgatttgtcttgatagctgctcctgcctttgggcaggggcttttttctgtctgccattcttgaggatggcggactctttcccttttgctctacgcccatacagaagtctaaagcagtgatctttactttcccccgtagccagtagctatgtcaccggcgaagtattacgggtaaatggggcccaccacacctaacttttgcggtactgtgcaagaatcctaattttctcctccctaattctggggagattttttttagccgtctaaatcccccgccatcaccgtggcgatcgccgttttggcattgtcctgaaattcccgcacattgactttgttgagcaggaaaatggagagaattaaccccagggcctg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z