BBa_K1476003 1 BBa_K1476003 PsaD deletion for Synechocystis sp. PCC 6803 2014-10-08T11:00:00Z 2015-05-08T01:10:40Z Organism: Cyanobacteria Genus and Strain: Synechocystis sp. PCC 6803 Source: http://genome.microbedb.jp/cyanobase/Synechocystis/genes/slr0737 Important genotype features: none This part deletes the PsaD subunit of photosystem I (PSI) in Synechocystis sp. PCC6803. false false _1856_ 0 21891 9 It's complicated false The part is designed to contained regions homologous to the psaD gene. As such we had to ensure that the homologous region was of sufficient length to allow for recombination. The part contains two regions homologous to psaD: the first region is homologous to the first 250 bases of psaD, the second region to the last 250 bases of the psaD gene. The centre of the part contains a kanamycin resistance gene, which becomes inserted in the middle of the chromosomal psaD gene during recombination. false Matthew Smith annotation2407396 1 KanR range2407396 1 281 1096 annotation2407382 1 psaD left range2407382 1 1 250 annotation2407395 1 psaD right range2407395 1 1177 1376 BBa_K1476003_sequence 1 agcagaatttggtgaaatgttacagttcttgacaaagccagatggtaaccgtaggtcagtggtaggattccctaaaataccttatgaaattccattttcatccctatgacagaactctctggacaaccgcctaaattcggtggcagcactggtggacttctctccaaagccaaccgggaagaaaagtatgccatcacctggaccagcgcctctgaacaagtttttgaaatgcccactggcggcgcggccatgagtgtcagccagtgtaacccgatgaggaatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaacacccagaagccgaactgggccagacaaccatcccagtggtgaagttcaatacctccaccccgccgatggcgttttccctgaaaaagttaacgaaggtcgggaagcccaaggtactaaaacccgccgcattggccaaaacccagaacctgtaaccatcaagttctccggtaaagccccctacgaggtctagttcctggctctacttgcttgcggaacttgcacaattggcagaagtttaactaacgggcgaatttgttaaaggcactactgaacctttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z