LOCUS       BBa_K1477014             432 bp    DNA     linear       28-Jun-2024
DEFINITION  Constitutive promoter +RBS+LacZ alpha+Term.
ACCESSION   BBa_K1477014
VERSION     BBa_K1477014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..295
                     /label=lacZ
     misc_feature    62..64
                     /label=START
     misc_feature    290..295
                     /label=STOP
     CDS             62..295
                     /label=BBa_I732006
     stem_loop       315..358
                     /label=stem_loop
     misc_feature    304..383
                     /label=BBa_B0010
     terminator      304..383
                     /label=BBa_B0010
     stem_loop       399..418
                     /label=T7 TE
     misc_feature    425..425
                     /label=stop
     polyA_site      419..432
                     /label=polya
     misc_feature    392..432
                     /label=BBa_B0012
     terminator      392..432
                     /label=BBa_B0012
     misc_feature    44..432
                     /label=BBa_I732020
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagaaagagg agaaatacta
       61 gatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      121 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      181 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      241 ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt aataatacta
      301 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      361 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      421 tctgcgttta ta
//