LOCUS       BBa_K1477030             725 bp    DNA     linear       22-May-2024
DEFINITION  T7 strong promoter + endolysin cassette
ACCESSION   BBa_K1477030
VERSION     BBa_K1477030.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             91..585
                     /label=T4 Endolysin
     CDS             75..588
                     /label=BBa_K112806
     stem_loop       608..651
                     /label=stem_loop
     misc_feature    597..676
                     /label=BBa_B0010
     terminator      597..676
                     /label=BBa_B0010
     stem_loop       692..711
                     /label=T7 TE
     misc_feature    718..718
                     /label=stop
     polyA_site      712..725
                     /label=polya
     misc_feature    685..725
                     /label=BBa_B0012
     terminator      685..725
                     /label=BBa_B0012
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaagag
       61 gagaaatact agagatactt aggaggtatt atgaatatat ttgaaatgtt acgtatagat
      121 gaaggtctta gacttaaaat ctataaagac acagaaggct attacactat tggcatcggt
      181 catttgctta caaaaagtcc atcacttaat gctgctaaat ctgaattaga taaagctatt
      241 gggcgtaatt gcaatggtgt aattacaaaa gatgaggctg aaaaactctt taatcaggat
      301 gttgatgctg ctgttcgcgg aatcctgaga aatgctaaat taaaaccggt ttatgattct
      361 cttgatgcgg ttcgtcgctg tgcattgatt aatatggttt tccaaatggg agaaaccggt
      421 gtggcaggat ttactaactc tttacgtatg cttcaacaaa aacgctggga tgaagcagca
      481 gttaacttag ctaaaagtag atggtataat caaacaccta atcgcgcaaa acgagtcatt
      541 acaacgttta gaactggcac ttgggacgcg tataaaaatc tataaagcta ctagagccag
      601 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      661 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      721 ttata
//