BBa_K1478002
1
BBa_K1478002
Top metal binding protein. Binds zinc, arsenic, copper and cadmium.
2014-09-17T11:00:00Z
2015-05-27T02:23:01Z
Arsenic (CAA06729), zinc (CAD88267) and cadmium (AAN06822) metallothioneins are full length. Copper AAA34542)domain is partial.
Multiple domain protein. Domains bind zinc, arsenic, copper and cadmium. Sequences derived from metallothioneins and transcription factors of different organisms. Arsenic, zinc and cadmium binding regions are full length metallothioneins. Zinc binding region derived from a transcritpion factor binding domain. Different protein binding regions are separated with glycerin-serine spacers to improve domain folding. Metal binding regions consists of disulfide bonds. For expression in E. coli disulfide bond forming strains are useful (e. g. Origami).
false
false
_1858_
4206
20973
9
It's complicated
false
Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
false
Fabian Fr??mling
BBa_K1478002_sequence
1
atggtggttatcaacggcgtcaagtacgcttgcgagacgtgtatccgtgggcatcgtgcagcacagtgtacacatacggacggaccacttcaaatgattcgtcgtaaaggacgtccttctaccacatgtggacacggtggaggcggttcaggcggaggtggctctggcggtggcggatcgtctgcgggtacaggttgtaagatatgggaagactgtaagtgcggagctgcctgctcatgcggagattcttgtacatgtggaacagtaaaaaagggtactacatcgcgtgcaggagcgggatgcccttgcggccctaaatgtaaatgtaccggacaaggatcatgcaactgtgtgaaagatgattgttgcggttgcggtaagggtggaggcggatcaggtggaggcggttcaggtggaggcggttcaagcgggtgtgacgataaatgcggttgtgcagtgccgtgccctggtggtacggggtgtcgttgcactagcgcacgttcaggagcagaacacaccacatgcgcgtgcggcgaacactgcggttgtaacccttgcgcgtgtggtcgtgagggcacacctagcggtcgtgaaaatcgtcggtcgaactgttcatgcggggcagcttgtaactgtgcttcgtgtgggagtacagcgggaggtggtggtagcggtggtggtgggtccggtggtggtggtagctcaagtgggaaaggcaagggtgagaagtgtacctctgcttgtcgttcagaaccttgtcagtgtggtagcaagtgtcaatgcggtgagggctgtacatgtgctgcctgcaagacttgtaattgtacaagtgatggttgcaaatgtggtaaggaatgtacgggaccagattcttgcaagtgcggatcatcttgcagctgtaagtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z