LOCUS BBa_K1479020 2649 bp DNA linear 23-Nov-2024 DEFINITION Easy BBK demo citing BBa_K1106000 ACCESSION BBa_K1479020 VERSION BBa_K1479020.1 FEATURES Location/Qualifiers misc_feature 1..66 /label=BBa_K145150 misc_feature 1..20 /label=Lux-box promoter 20..25 /label=-35 misc_feature 23..40 /label=OR2 promoter 42..47 /label=-10 misc_feature 46..63 /label=OR1 misc_feature 67..81 /label=BBa_B0030 RBS 67..81 /label=RBS-1Strong misc_feature 67..81 /label=BBa_B0030 RBS 74..78 /label=RBS misc_feature 82..762 /label=BBa_E0010 misc_feature 763..842 /label=BBa_B0010 misc_feature 763..842 /label=BBa_B0010 stem_loop 774..817 /label=stem_loop misc_feature 843..883 /label=BBa_B0012 misc_feature 843..883 /label=BBa_B0012 stem_loop 850..869 /label=T7 TE polyA_site 870..883 /label=polya misc_feature 876..876 /label=stop misc_feature 884..924 /label=BBa_B0012 misc_feature 884..924 /label=BBa_B0012 stem_loop 891..910 /label=T7 TE polyA_site 911..924 /label=polya misc_feature 917..917 /label=stop misc_feature 925..1004 /label=BBa_B0010 misc_feature 925..1004 /label=BBa_B0010 stem_loop 936..979 /label=stem_loop misc_feature 1005..1685 /label=BBa_E0010 misc_feature 1686..1700 /label=BBa_B0030 RBS 1686..1700 /label=RBS-1Strong misc_feature 1686..1700 /label=BBa_B0030 RBS 1693..1697 /label=RBS misc_feature 1701..1766 /label=BBa_K145150 misc_feature 1701..1720 /label=Lux-box promoter 1720..1725 /label=-35 misc_feature 1723..1740 /label=OR2 promoter 1742..1747 /label=-10 misc_feature 1746..1763 /label=OR1 misc_feature 1767..1832 /label=BBa_K145150 misc_feature 1767..1786 /label=Lux-box promoter 1786..1791 /label=-35 misc_feature 1789..1806 /label=OR2 promoter 1808..1813 /label=-10 misc_feature 1812..1829 /label=OR1 misc_feature 1833..1847 /label=BBa_B0030 RBS 1833..1847 /label=RBS-1Strong misc_feature 1833..1847 /label=BBa_B0030 RBS 1840..1844 /label=RBS misc_feature 1848..2528 /label=BBa_E0010 misc_feature 2529..2608 /label=BBa_B0010 misc_feature 2529..2608 /label=BBa_B0010 stem_loop 2540..2583 /label=stem_loop misc_feature 2609..2649 /label=BBa_B0012 misc_feature 2609..2649 /label=BBa_B0012 stem_loop 2616..2635 /label=T7 TE polyA_site 2636..2649 /label=polya misc_feature 2642..2642 /label=stop ORIGIN 1 acctgtagga tcgtacaggt ttactaaaga ttcctttagt ttataattta agtgttcttt 61 aatttcatta aagaggagaa aatggcctcc tccgaggacg tcatcaagga gttcatgcgc 121 ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 181 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc 241 ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa ggtgtacgtg 301 aagcaccccg ccgacatccc cgactacaag aagctgtcct tccccgaggg cttcaagtgg 361 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg 421 caagacggct ccttcatcta caaggtgaag ttcatcggcg tgaacttccc ctccgacggc 481 cccgtaatgc agaagaagac tatgggctgg gaggcctcca ccgagcgcct gtacccccgc 541 gacggcgtgc tgaagggcga gatccacaag gccctgaagc tgaaggacgg cggccactac 601 ctggtggagt tcaagtccat ctacatggcc aagaagcccg tgcagctgcc cggctactac 661 tacgtggact ccaagctgga catcacctcc cacaacgagg actacaccat cgtggagcag 721 tacgagcgcg ccgagggccg ccaccacctg ttcctgtaat aaccaggcat caaataaaac 781 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 841 tctcacactg gctcaccttc gggtgggcct ttctgcgttt atatcacact ggctcacctt 901 cgggtgggcc tttctgcgtt tataccaggc atcaaataaa acgaaaggct cagtcgaaag 961 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcatggcc tcctccgagg 1021 acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg 1081 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc 1141 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc 1201 agtacggctc caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt 1261 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga 1321 ccgtgaccca ggactcctcc ctgcaagacg gctccttcat ctacaaggtg aagttcatcg 1381 gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa gactatgggc tgggaggcct 1441 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac aaggccctga 1501 agctgaagga cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc 1561 ccgtgcagct gcccggctac tactacgtgg actccaagct ggacatcacc tcccacaacg 1621 aggactacac catcgtggag cagtacgagc gcgccgaggg ccgccaccac ctgttcctgt 1681 aataaattaa agaggagaaa acctgtagga tcgtacaggt ttactaaaga ttcctttagt 1741 ttataattta agtgttcttt aatttcacct gtaggatcgt acaggtttac taaagattcc 1801 tttagtttat aatttaagtg ttctttaatt tcattaaaga ggagaaaatg gcctcctccg 1861 aggacgtcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc 1921 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca 1981 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt 2041 tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac tacaagaagc 2101 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg 2161 tgaccgtgac ccaggactcc tccctgcaag acggctcctt catctacaag gtgaagttca 2221 tcggcgtgaa cttcccctcc gacggccccg taatgcagaa gaagactatg ggctgggagg 2281 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc cacaaggccc 2341 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac atggccaaga 2401 agcccgtgca gctgcccggc tactactacg tggactccaa gctggacatc acctcccaca 2461 acgaggacta caccatcgtg gagcagtacg agcgcgccga gggccgccac cacctgttcc 2521 tgtaataacc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 2581 tatctgttgt ttgtcggtga acgctctctc acactggctc accttcgggt gggcctttct 2641 gcgtttata //