LOCUS       BBa_K1480000            3228 bp    DNA     linear       22-Nov-2024
DEFINITION  Promoter + RBS + TALEN + Terminator
ACCESSION   BBa_K1480000
VERSION     BBa_K1480000.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             65..3091
                     /label=BBa_K1480001
     stem_loop       3111..3154
                     /label=stem_loop
     misc_feature    3100..3179
                     /label=BBa_B0010
     terminator      3100..3179
                     /label=BBa_B0010
     stem_loop       3195..3214
                     /label=T7 TE
     misc_feature    3221..3221
                     /label=stop
     polyA_site      3215..3228
                     /label=polya
     misc_feature    3188..3228
                     /label=BBa_B0012
     terminator      3188..3228
                     /label=BBa_B0012
     terminator      3100..3228
                     /label=BBa_B0015
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagattaaag aggagaaata
       61 ctagatggac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
      121 tgacgatgac aagatggccc ccaagaagaa gaggaaggtg ggcattcacg gggtacccgc
      181 cgctgtggat ctacgcacgc tcggctacag ccagcagcaa caggagaaga tcaaaccgaa
      241 ggttcgttcg acagtggcgc agcaccacga ggcactggtc ggccatgggt ttacacacgc
      301 gcacatcgtt gcgctcagcc aacacccggc agcgttaggg accgtcgctg tcaagtatca
      361 ggacatgatc gcagcgttgc cagaggcgac acacgaagcg atcgttggcg tcggcaaaca
      421 gtggtccggc gcacgcgccc tggaggcctt gctcacggtg gcgggagagt tgagaggtcc
      481 accgttacag ttggacacag gccaacttct caagattgca aaacgtggcg gcgtgaccgc
      541 agtggaggca gtgcatgcat ggcgcaatgc actgacgggg gcccccctga accttactcc
      601 cgaacaagta gtagcgatag ccagtaataa cggaggtaaa caagccttgg agacggtcca
      661 aaggttgctc ccggtcttgt gtcaggcaca tgggctgacg cctcaacagg tcgtcgcgat
      721 agcgtctaat aatggaggaa agcaagctct ggaaaccgtc cagcgactcc ttccggttct
      781 gtgccaggct catggtctga ctccgcagca agtcgttgct atagcgtcca acatcggagg
      841 caaacaggcc ctggagaccg tgcagcggtt gttgcctgtg ctttgccaag cccacgggct
      901 tacgcctgag caagtggtgg cgattgccag taacaacggc ggcaaacaag cccttgagac
      961 tgtgcagagg ctcttgccgg tactctgcca agcacacggc ttgacccccg agcaggttgt
     1021 agccatagct agtcacgacg ggggtaagca agcgttggaa acggtgcaag cacttctccc
     1081 cgttctctgt caagcgcatg gacttacccc ggaacaggtg gtcgccattg caagccatga
     1141 tggaggaaag caggcgctcg aaacagtcca ggcacttttg cccgtacttt gtcaagctca
     1201 cggtctcacc ccggaacagg tggtagccat tgcatctaac atcggaggta agcaagcatt
     1261 ggaaacggtt caggccctgt tgcctgtact ttgccaggcg cacggtctga cacctgagca
     1321 ggttgtcgcc atcgctagca acggaggtgg gaaacaggca cttgaaactg tgcagaggct
     1381 tctgccggtg ctgtgccaag cgcatggcct tacacccgag caagtagtgg ctattgcgag
     1441 tcatgatgga ggcaagcaag cgctggagac tgtccaacga cttcttccgg tcttgtgtca
     1501 ggcacatgga ttgacccctc aacaagtcgt ggcgatagct agcaacggcg gtggaaaaca
     1561 ggccctcgaa accgtccagc gactgctccc cgtactgtgt caagcccatg gacttacccc
     1621 agaacaagtt gtggcgattg cctctaacaa tggtgggaag caagctcttg agacggtgca
     1681 ggcgttgttg cccgtgcttt gtcaagctca cgggctcacg ccagagcaag tggtcgctat
     1741 cgcgagtaat aaagggggca aacaagcctt ggagacagtg caaaggctcc tgccagtgct
     1801 ctgccaggct catggtttga cacccgaaca ggtagttgca atagcgagtc atgatggcgg
     1861 aaagcaagct cttgaaactg tgcagcggct gttgcctgta ctgtgtcaag cccacgggct
     1921 gacaccggaa caagttgtag cgatcgctag ccacgatggc gggaaacaag ctctggaaac
     1981 ggtacagaga ctcctcccag tgctttgtca ggcacacggc ctcacgccag agcaggttgt
     2041 cgccatcgcg tcaaacaatg gtggaaagca ggccctggag acagtccaac ggttgctgcc
     2101 ggtcctttgc caggctcacg ggttgacccc ccagcaggtc gtggccattg cctcaaacaa
     2161 gggcggtagg ccagcattgg agacggtgca gaggcttctg cctgtgctct gccaagcgca
     2221 tggactcacc cccgagcaag tggttgctat cgcaagtaac aacggaggga aacaagcgct
     2281 cgaaaccgtg caaaggttgc tccccgttct ctgtcaggcg cacggtctta cgccacaaca
     2341 ggtggtggcg attgcatcta atggaggcgg acgccctgcc ttggagagca ttgtggccca
     2401 gctgtccagg ccggaccctg ccctggccgc gttaaccaac gaccacctcg tcgccttggc
     2461 ctgcctcggc ggaggttctg gcggcagcgg atcccagctg gtgaagagcg agctggagga
     2521 gaagaagtcc gagctgcggc acaagctgaa gtacgtgccc cacgagtaca tcgagctgat
     2581 cgagatcgcc aggaacagca cccaggaccg catcctggag atgaaggtga tggagttctt
     2641 catgaaggtg tacggctaca ggggaaagca cctgggcgga agcagaaagc ctgacggcgc
     2701 catctataca gtgggcagcc ccatcgatta cggcgtgatc gtggacacaa aggcctacag
     2761 cggcggctac aatctgccta tcggccaggc cgacgagatg cagagatacg tggaggagaa
     2821 ccagacccgg aataagcaca tcaaccccaa cgagtggtgg aaggtgtacc ctagcagcgt
     2881 gaccgagttc aagttcctgt tcgtgagcgg ccacttcaag ggcaactaca aggcccagct
     2941 gaccaggctg aaccacatca ccaactgcaa tggcgccgtg ctgagcgtgg aggagctgct
     3001 gatcggcggc gagatgatca aagccggcac cctgacactg gaggaggtgc ggcgcaagtt
     3061 caacaacggc gagatcaact tcagatctta atactagagc caggcatcaa ataaaacgaa
     3121 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     3181 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//