LOCUS       BBa_K1480002             836 bp    DNA     linear       03-May-2024
DEFINITION  pTet + RBS + mRFP1 + Transcriptional terminator
ACCESSION   BBa_K1480002
VERSION     BBa_K1480002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..758
                     /label=mrfp1
     misc_feature    765..789
                     /label=Help:Barcodes
     CDS             84..789
                     /label=BBa_E1010
     misc_feature    798..836
                     /label=B1006
     stem_loop       807..828
                     /label=modified thr terminator
     polyA_site      829..836
                     /label=PolyA
     polyA_site      798..806
                     /label=PolyA
     terminator      798..836
                     /label=BBa_B1006
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc
      121 gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag
      181 gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc
      241 cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg
      301 ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat
      361 gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc
      421 tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg
      481 gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg
      541 aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact
      601 acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt
      661 acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac
      721 agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt
      781 gtagatcgct actagagaaa aaaaaacccc gcccctgaca gggcggggtt tttttt
//