LOCUS BBa_K1480002 836 bp DNA linear 02-Dec-2024 DEFINITION pTet + RBS + mRFP1 + Transcriptional terminator ACCESSION BBa_K1480002 VERSION BBa_K1480002.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..77 /label=RBS-1\Strong RBS 70..74 /label=RBS misc_feature 63..77 /label=BBa_B0030 RBS 63..77 /label=BBa_B0030 CDS 84..758 /label=mrfp1 misc_feature 765..789 /label=Help:Barcodes CDS 84..789 /label=BBa_E1010 misc_feature 798..836 /label=B1006 stem_loop 807..828 /label=modified thr terminator polyA_site 829..836 /label=PolyA polyA_site 798..806 /label=PolyA terminator 798..836 /label=BBa_B1006 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agattaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc 121 gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag 181 gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc 241 cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg 301 ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat 361 gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc 421 tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg 481 gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg 541 aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact 601 acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt 661 acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac 721 agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt 781 gtagatcgct actagagaaa aaaaaacccc gcccctgaca gggcggggtt tttttt //