LOCUS       BBa_K1481004            1967 bp    DNA     linear       22-Dec-2024
DEFINITION  sfGFP with MLRR tag under arabinose promoter
ACCESSION   BBa_K1481004
VERSION     BBa_K1481004.1  
FEATURES             Location/Qualifiers
     CDS             1..879
                     /label=AraC
     promoter        880..1217
                     /label=pBAD
     CDS             1218..1967
                     /label=his-sfGFP
     misc_feature    909..924
                     /label=O1 signal
     misc_feature    1113..1155
                     /label=AraC binding site
     misc_feature    1066..1087
                     /label=O2 signal
     misc_feature    1191..1217
                     /label=5'-UTR
     misc_feature    1230..1247
                     /label=his-tag
     misc_feature    1111..1121
                     /label=CRP binding site
     misc_feature    1153..1157
                     /label=-35 signal
     misc_feature    1177..1181
                     /label=-10 signal
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg
      181 actaatgcgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttggatggag tgaaacgatg ctgctgcgtc atcatcatca tcatcatatg cgtaaaggcg
     1261 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
     1321 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
     1381 agttcatctg tactactggt aaactgccgg taccttggcc gactctggta acgacgctga
     1441 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
     1501 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
     1561 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
     1621 tgaaaggcat tgactttaaa gaagacggca atatcctggg ccataagctg gaatacaatt
     1681 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
     1741 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
     1801 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
     1861 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
     1921 ccgcagcggg catcacgcat ggtatggatg aactgtacaa ataataa
//