LOCUS       BBa_K1481005            2964 bp    DNA     linear       13-May-2024
DEFINITION  Strobable rhamnose AND temperature operated transcriptor SR-latch
ACCESSION   BBa_K1481005
VERSION     BBa_K1481005.1  
FEATURES             Location/Qualifiers
     promoter        1..124
                     /label=pRha
     misc_feature    14..35
                     /label=CRP binding site
     misc_feature    36..84
                     /label=RhaS binding site
     misc_feature    83..88
                     /label=-35 signal
     misc_feature    106..111
                     /label=-10 signal
     RBS             125..163
                     /label=U10-U
     CDS             164..1669
                     /label=Bxb1
     promoter        1670..2150
                     /label=radB
     CDS             2151..2900
                     /label=histag-sfGFP
     misc_feature    2163..2180
                     /label=his-tag
     misc_feature    2901..2964
                     /label=attPR4
     misc_feature    1670..1726
                     /label=T5 terminator
ORIGIN
        1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
       61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg
      121 aaccggatcc tctccttact attcttcaga aggagatata cccgtgagag ccctggtagt
      181 catccgcctg tcccgcgtca ccgatgctac gacttcaccg gagcgtcagc tggagtcttg
      241 ccagcagctc tgcgcccagc gcggctggga cgtcgtcggg gtagcggagg atctggacgt
      301 ctccggggcg gtcgatccgt tcgaccggaa gcgcagaccg aacctggccc ggtggctagc
      361 gttcgaggag caaccgtttg acgtgatcgt ggcgtaccgg gtagatcggt tgacccgatc
      421 gatccggcat cttcagcagc tggtccactg ggccgaggac cacaagaagc tggtcgtctc
      481 cgcgaccgaa gcgcacttcg atacgacgac gccgtttgcg gcggtcgtca tcgcgcttat
      541 gggaacggtg gcgcagatgg aattagaagc gatcaaagag cggaaccgtt cggctgcgca
      601 tttcaatatc cgcgccggga aataccgagg atccctgccg ccgtggggat acctgcctac
      661 gcgcgtggac ggggagtggc ggctggtgcc ggaccctgtg cagcgagagc gcatcctcga
      721 ggtgtatcac cgcgtcgtcg acaaccacga gccgctgcat ctggtggccc acgacctgaa
      781 ccggcgtggt gtcctgtcgc cgaaggacta cttcgcgcag ctgcaaggcc gcgagccgca
      841 gggccgggag tggtcggcta ccgcgctgaa gcgatcgatg atctccgagg cgatgctcgg
      901 gtacgcgact ctgaacggta agaccgtccg agacgacgac ggagccccgc tggtgcgggc
      961 tgagccgatc ctgacccgtg agcagctgga ggcgctgcgc gccgagctcg tgaagacctc
     1021 ccgggcgaag cccgcggtgt ctaccccgtc gctgctgctg cgggtgttgt tctgcgcggt
     1081 gtgcggggag cccgcgtaca agttcgccgg gggaggacgt aagcacccgc gctaccgctg
     1141 ccgctcgatg gggttcccga agcactgcgg gaacggcacg gtggcgatgg ccgagtggga
     1201 cgcgttctgc gaggagcagg tactggatct gctcggggac gcggagcgtc tggagaaagt
     1261 ctgggtagcg ggctcggact ccgcggtcga actcgcggag gtgaacgcgg agctggtgga
     1321 cctgacgtcg ctgatcggct ccccggccta ccgggcgggc tctccgcagc gagaagcact
     1381 ggatgcccgt attgcggcgc tggccgcgcg gcaagaggag ctggagggcc tggaggctcg
     1441 cccgtctggc tgggagtggc gcgagaccgg gcagcggttc ggggactggt ggcgggagca
     1501 ggacaccgcg gcaaagaaca cctggcttcg gtcgatgaac gttcggctga cgttcgacgt
     1561 ccgcggcggg ctgactcgca cgatcgactt cggggatctt caggagtacg agcagcatct
     1621 caggctcggc agcgtggtcg aacggctaca caccgggatg tcgtaataag gaaacacaga
     1681 aaaaagcccg cacctgacag tgcgggcttt ttttttcgac caaaggcaca gctaacacca
     1741 cgtcgtccct atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg
     1801 cataaggctc gtataatata ttcagggaga ccacaacggt ttccctctac aaataatttt
     1861 gtttaacttt agttgcccat gaccatgccg aagcagtggt agaagggcac cggcagacac
     1921 tcggccggct tgtcgacgac ggcggtctcc gtcgtcagga tcatccgggc ggcatcaaat
     1981 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2041 cgctctcctg agtaggacaa atccgccgcc ctagagtcgg ggtttgtacc gtacaccact
     2101 gagaccgcgg tggttgacca gacaaaccac gacaaagagg agaaatcaat atgggcagca
     2161 gccatcatca tcatcatcat atgcgtaaag gcgaagagct gttcactggt gtcgtcccta
     2221 ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg
     2281 aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc
     2341 cggtaccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt
     2401 atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc
     2461 aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat
     2521 ttgaaggcga taccctggta aaccgcattg agctgaaagg cattgacttt aaagaagacg
     2581 gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg
     2641 ccgataaaca aaaaaatggc attaaagcga attttaaaat tcgccacaac gtggaggatg
     2701 gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc
     2761 tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga
     2821 aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg
     2881 atgaactgta caaataataa gcatgttccc caaagcgata ccacttgaag cagtggtact
     2941 gcttgtgggt acactctgcg ggtg
//