LOCUS BBa_K1484317 4061 bp DNA linear 29-Nov-2024 DEFINITION 4-Hydroxybenzalacetone Generator ACCESSION BBa_K1484317 VERSION BBa_K1484317.1 FEATURES Location/Qualifiers misc_feature 1..1042 /label=AraC promoter 1043..1210 /label=pBAD RBS 1211..1234 /label=RBS CDS 1235..2863 /label=4-coumarate:coenzyme-A ligase RBS 2864..2906 /label=RBS CDS 2907..4061 /label=Benzalacetone Synthase ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc aggtagagaa agaggagaaa tacgatggag aaagatacaa aacaggttga 1261 cataattttc cgatcaaaac tccctgatat ttacatccct aaccatcttc ctttacactc 1321 ctactgtttc gaaaacattt ccgagttcag ttctcgtcct tgtttaatca atggcgccaa 1381 caaacaaatt tatacgtatg ctgatgttga actcaattca agaaaagttg ctgctggtct 1441 tcacaaacaa gggattcaac caaaggatac aataatgatc ctattgccta actccccaga 1501 atttgtgttt gctttcattg gtgcatcgta cctcggagct atttctacaa tggccaatcc 1561 tttgtttact cctgctgagg ttgtgaagca agccgaggct tctagtgcta agatcattgt 1621 cacacaagcg tgtcatgtta acaaagtgaa agattatgca tttgagaatg atgtgaagat 1681 catatgcatc gactcggcgc cggagggttg tctccacttc tccgtgctaa ctcaggctaa 1741 tgagcacgat attcctgagg ttgaaattca acctgacgat gtggtggcgt tgccatactc 1801 ctccgggacg acgggattac ctaaaggagt gatgttgacg cacaagggac ttgtgacaag 1861 cgtcgcacaa caagtcgacg gtgaaaatcc gaatttgtat atccatagcg aggacgtgat 1921 gctttgtgtc ttgcccttgt tccatatcta ttcactcaac tccgttttgc tttgtggatt 1981 aagggtggga gcagcgattt tgattatgca gaaatttgat attgtttctt tcttggagtt 2041 gatacaaagt tacaaggtga caatagggcc gtttgtacca cctattgttt tggtcattgc 2101 taagagtcct atggttgatg attatgatct ttcatcagta agaaccgtca tgtctggggc 2161 tgcaccatta ggaaaggagc ttgaagatac tgttcgagcc aaatttccta atgctaaact 2221 tggtcagggt tatggtatga cagaagctgg accagtgttg gctatgtgct tggcatttgc 2281 aaaagaaccc tttgaaataa aatcaggggc atgtggaaca gttgtgagaa atgctgaaat 2341 gaaaattgtg gatcctaaaa ctggtaattc tcttcccaga aatcaatctg gagaaatttg 2401 cattagagga gaccagatca tgaaaggcta cctgaatgat ccagaggcca cagcaagaac 2461 aatagacaaa gaagggtggt tatatactgg tgacattggc tacattgatg atgacgacga 2521 gcttttcatt gttgatcgat taaaggaact gatcaaatac aaaggatttc aagtcgcacc 2581 tgctgagctc gaagctctcc ttctcaacca tcccaacatt tctgatgctg ctgttgtccc 2641 catgaaggac gagcaagcag gagaagttcc agtggctttt gttgttagat ccaacggatc 2701 caccattact gaagatgaag tcaaagattt tatttcaaag caggtgatat tttataagag 2761 gataaagcgg gtatttttcg tggatgctat tcctaaatct ccatctggca aaatccttcg 2821 aaaagatttg agagctaaac tggctgctgg gcttccaaat taagagatca tagacacgag 2881 aacactagga cctaaggagg atagatatgg caactgagga gatgaagaaa ttggccaccg 2941 tgatggccat tggcacggcc aaccctccga actgctacta ccaggccgac tttcccgact 3001 tctacttccg cgtcaccaac agcgaccacc tcatcaacct caagcaaaag ttcaagcgcc 3061 tttgtgaaaa ctcaaggatt gagaagcgtt accttcatgt gaccgaagag attctcaagg 3121 aaaacccaaa cattgctgcc tacgaggcaa cctcgttgaa tgtaagacac aaaatgcaag 3181 tgaaaggagt tgcagagctt gggaaagagg ctgccctcaa ggccatcaaa gaatggggcc 3241 aacccaagtc caagatcaca catctcatcg tgtgttgcct agccggcgtt gacatgcccg 3301 gcgcggatta tcaactcact aagcttcttg accttgaccc ttccgtcaag cgttttatgt 3361 tttaccacct aggatgctac gctggtggca ctgtccttcg ccttgcaaag gacatagcgg 3421 agaacaacaa gggagctcgt gttctcatcg tttgctcaga gatgacaaca acttgttttc 3481 gtgggccatc tgaaacccat ctggactcca tgataggcca agcaatatta ggcgatgggg 3541 ctgccgctgt catagttggc gcagatccag acctaaccgt tgagaggccc atattcgagt 3601 tggtttccac agcccagact attgtacccg aatcccatgg tgcaattgag ggccacttgc 3661 ttgaatctgg actcagtttc catttgtaca agaccgttcc tacactaatc tctaacaaca 3721 ttaaaacttg cctttctgat gctttcactc ctctaaacat tagcgattgg aactctcttt 3781 tctggatcgc acaccctggt ggtcctgcca tcctagacca agttactgct aaggttggtc 3841 ttgaaaagga gaaactcaag gtaactagac aagtgttgaa ggactatgga aacatgtcga 3901 gtgctacggt gtttttcatc atggatgaga tgaggaagaa gtcactcgaa aacggtcaag 3961 caaccactgg agaagggctc gagtggggtg ttttgtttgg gttcgggcct ggaatcaccg 4021 ttgaaactgt agtgctacgc agtgtgcccg taattagcta g //