BBa_K1484327
1
BBa_K1484327
P_NiI10, marchantia promoter
2014-10-09T11:00:00Z
2015-05-08T01:10:41Z
This part was found by screening the genome of the Marchantia polymorpha Cam strain maintained by the Haseloff lab for homologues to Nitrate Transporter proteins in A. Thaliana and B. Napus, known respectively as AtNRT2.1 or ACH1 and BnNRT1.2 or RCH2. Transcription is thought to be induced by nitrate (NO-3).
Matches to the protein CDS sequence were found using Geneious to perform a tblastn search on the genome scaffolds. Predicted genes that contained hits graded above 30% and with at least 40% congruence to mRNA transcript sequences were shortlisted. The best gene candidates (judged according to number and distribution of hits along its length, and supporting mRNA sequence) formed the basis for our predicted promoters. This part was isolated from a 2kb region upstream of the first ATG of such a gene
This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this.
We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.
false
false
_1864_
0
21029
9
Not in stock
false
The removal of illegal restriction sites was considered but not completed due to direct interaction of the DNA with regulatory proteins. Also, it was ensured that the region selected for this part was directly upstream of the first ATG of the gene used to identify the promoter sequence. This was so that the 5' UTR, that is vital in plants, was maintained.
false
Angelina Munabi
BBa_K1484327_sequence
1
aatggatccacaccttgcacttgacgaaatttacattctaagctgggcactagtctcattcgaatgtagggtcatctatttactgtttgcgtacgaatttgtcaacacgttgatgttcgtactcctcgctctccgaactatttttgagttactctgatcttgcaaaccaaatctttatcaattacgtggaaaaccgttctttagctacctactgaacacacaactgatccaaatagaagaaaattgtcattgccaaaacactgtggagggcaaagatagaaggcatcgtacatcaaactcagttgcagtcgatacacaaccgtgacgtaaagagtgaagttgatattaccagagtccggttgccgggcaatggttgactctatccccagtaaggatgtcctccactgtgttgaagtttagtttgataggagaaccgatgcccaacttcagggcatgcacaacagcagggccgacaatgaaggcccgaatctttacagcaatgtcgttggccaaatgctagttccggtgtacaactggtcatatcaaaactgactttaacagacttcaattcgaattcatctcgttgaagcttctcacgagttgggatgcgtcaccagcagatacagtaccggcatctccaacgacaaaagttgctctgggaagctcttgtgcagctaactaaacttccgattcctacgtacccgctggccagcctttgcccactacagcatcagttcgaagtgagccatggatttcgaagcacagtgcccatgaaatgacttggacaaaaccttgagccttctagtttagcagagcaaacgccgttccaggagagatgggtccagcagcgcagtagaggatgagagaaacttagagaaggtgaagggtcagaacacatgtctgataaacttcgaccttctgatcttttgatcgatcgttcgttcgttctggtaatgcgatcacaacgaaagaggaggttaattagcgttacagataggttcgctcaaaccaaggaagaaaagtagaaacgcagccgaaggtacgtggcgtaccgggtagacaggtaaagctcctagctatggtacaggggtattgttatgactggtaccaaccggcaaccacaagtgagtcgaagcgagtgcgcaagcaggaggacgatgccacatggacacctgccgtccgtgcagcatgtagacagcagcatagggcggaaggcgggcgattagttagctgctgctgctcagctccgggtcaggattgcattcatgcccttctcgctctgcctaacgtcagattcaccagagaccgtatgtagagagtttcagcagctttgttgaccggatccatacacttgagacaatctgtgagtgattttcatgtgcacgaaataataaacgcctcagactctgagtcccagcccagacagacagacctccaagggaaccaagtgaagcaccgttatacgctgttcagatacaaggagagcatgattccagagtgcagaagccgcaggttatggttttggacaagcgatatgattgcatatgaagggaagtgaggtttgaagactgatcatctgatctgattgccgatggattccgttcactacctaatggcgctctacatacgaaatgaatacttttcatacgaaagtttctgctctgcccaagctcaacctccactttccgcctcattggcgtgttcaacgaccatatgacgaactccctgtttggatataaaactctcaacgtgtcatcagtagattggaaacagtgacgactgtcccgttccatcctctctctctcctgctccctaagatagatctccttttatccactgtcatgctcgaagctactagacctcttttcatgcaaacccatcagtataaaggccgcgccggggccttcaatctttatgccattactatcttgtgtgttattctctctcaggccaggcccctggccagagcgaactctcgcagcacccattccttacgtcctcggtcttcacctgcaggttcgacgactctctgagttgattag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z