BBa_K1484336 1 BBa_K1484336 P_NiI20, marchantia promoter 2014-10-09T11:00:00Z 2015-05-08T01:10:41Z This part was found by screening the genome of the Marchantia polymorpha Cam strain maintained by the Haseloff lab for homologues to Nitrate Transporter proteins in the following organisms and including corresponding genes: A. thaliana - AtNRT2.1, AtNRT2.2; N. plumbaginifolia - NpNRT2; C. reinhardtii - CrNRT2.1, CrNRT2.3; P. patens - NRT2.1, NRT2.2, NRT2.3, NRT2.4, NRT2.5 Transcription is thought to be induced by nitrates. Matches to the protein CDS sequence were found using Geneious to perform a tblastn search on the genome scaffolds. Predicted genes that contained hits graded above 30% and with at least 40% congruence to mRNA transcript sequences were shortlisted. The best gene candidates (judged according to number and distribution of hits along its length, and supporting mRNA sequence) formed the basis for our predicted promoters. This part was isolated from a 2kb region upstream of the first ATG of such a gene. This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome. false false _1864_ 0 21029 9 Not in stock false The removal of illegal restriction sites was considered but not completed due to direct interaction of the DNA with regulatory proteins. Also, it was ensured that the region selected for this part was directly upstream of the first ATG of the gene used to identify the promoter sequence. This was so that the 5' UTR, that is vital in plants, was maintained. false Angelina Munabi BBa_K1484336_sequence 1 tgtgtgtgtgtgtggggtaagttgccctggttctgaaacgtcatttattctagaaaatcagaggatttcataccaacactgcagttggttacttacttagcatgctctgattatatcggttcctgaagaaaatttgcgagagtattcatatgaaggaagtgctcttcctagtaaaacaattattttcgctccatacagtgatgtataaaatgcaaagttgcaatttattcaatcgtggtggttattgtcaactgaagaggcgaggaggggatgaaggcctcacacagaggaatccatccaacatgatcccgtcgtcaggctaactttaagccggaattgtcaacattgagctccagactgctcggtggccacttacagtagcatgtagcttgcggagcatttgtcgctgcaatgttcgcggagggaaaatacggcagaagtgaagaggggaaaagcctacagtagctggctggcgtacgtgggtccatgtctggaagcttagtggcttcgcgagcagtctacggtagggcgacttgtaggcgaaaggtagaagtggaagtagaagaagaagaagaactagggagtatgtgtatgcgacttaccatctggtttgttaaggtagagtgaccagggaacaatccttcaaaggccttaagggccagtcggcacgaattcgacgtctaaaaggtcagggtctattgcattgagtgatatgcaaaagctgcagggtaaggtgaatccatccatggtgcagagaggatcgaattgtagatgggggcccaggaaacacgagcagtgtgctgaggagtagagagacctggtggaccctcggaattcaattatccccagaagctatctatggtggaccctcggaactcaggatcccctgaagtaaatcacactgctaattgctgcgactgtcatcgtccttcgccgtctccactataaaggcgcctcggtcgtcaaacatttcaagcctgcctcggttgtcaatctatcctagtgcgctaaacagaatatagtccaggtctggtgagcactgaagttccaagagtgtattaagtcactcacatccgtagacggtcgcaggtttctagtctttcagtgtacagccgatgctgaaggtccgagctcgaaaacctctagaaagagccaattcgtaagaatttttgagctcatgctaggattcattacatagtagcgtagcatcatattatagccacaggaagctcagacggaccgacagaccctgagcgagaggagagtccagctgcagcgatggcaccagagggtacccgggcggacgagggagagggtcagcccggctcgtccatgcacggagtgacaggacatgagccgttcccattcatggtggaaacggtagctgaggaagcgacggatttgaaagggaggaagttctcgttacctgtggattcagagcacaaggccaggaagcttaagctgtattcgttcgctccacctcacatgctgacatttcacctgtcgtggatatcgttcatgacctgcttcttctccacattcgccccccctccgctcatgcccgtcatccgcgacaatttgggcctcacgaaaagcgatattgccaatgctggaatcgcttcggtctccggctccatcatctctcgtctcctgatgggcaccatatgcgatctggccggacctcgatatgggtgcgccattctcatcatggtcacagctcctgcagtgttctgtatgcccctcgtttccaccatcaacggattcatcatggtgcgattcttcatcagcttcgccctggcaactttcgtctcgtgccagttctggatgagctccatgttcaatgggcagattgtggggctggccaacgggacggcagctggatggggaaacatgggtggaggcatcacgcagctcgtaatgcctttggtctacgatctcattcataagaaattcggatcagagatcttcactgcatggcgtctggctttcttcgtgccagg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z