BBa_K1486008
type: ComponentDefinition
persistent identity: BBa K1486008
display id: BBa_K1486008
version: 1
was generated by: iGEM to SBOL conversion
description: CxpR & Split IFP1.4 [Cterm + Cterm][1]
created: 2014-08-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - IFP[1]-CpxR + IFP[2]-CpxR (BBa_K1286009) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
star: false
creator: iGem EPFL 2014
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K1486004
component: BBa_K1486000
component: BBa_B0034
component: BBa_K1486052
component: BBa_K1486007
component: BBa_I0500
component: BBa_K1486006
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486052
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486008_sequence/1

BBa_K1486004
type: Component
persistent identity: component2418053
display id: component2418053
version: 1
top level: BBa_K1486008
definition: BBa_K1486004
access: public

BBa_K1486000
type: Component
persistent identity: component2418052
display id: component2418052
version: 1
top level: BBa_K1486008
definition: BBa_K1486000
access: public

BBa_B0034
type: Component
persistent identity: component2418048
display id: component2418048
version: 1
top level: BBa_K1486008
definition: BBa_B0034
access: public

BBa_K1486052
type: Component
persistent identity: component2418046
display id: component2418046
version: 1
top level: BBa_K1486008
definition: BBa_K1486052
access: public

BBa_K1486007
type: Component
persistent identity: component2418057
display id: component2418057
version: 1
top level: BBa_K1486008
definition: BBa_K1486007
access: public

BBa_I0500
type: Component
persistent identity: component2418034
display id: component2418034
version: 1
top level: BBa_K1486008
definition: pBad/araC
access: public

BBa_K1486006
type: Component
persistent identity: component2418045
display id: component2418045
version: 1
top level: BBa_K1486008
definition: BBa_K1486006
access: public

BBa_K1486004
type: Component
persistent identity: component2418041
display id: component2418041
version: 1
top level: BBa_K1486008
definition: BBa_K1486004
access: public

BBa_K1486000
type: Component
persistent identity: component2418040
display id: component2418040
version: 1
top level: BBa_K1486008
definition: BBa_K1486000
access: public

BBa_B0034
type: Component
persistent identity: component2418036
display id: component2418036
version: 1
top level: BBa_K1486008
definition: BBa_B0034
access: public

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2418034
display id: annotation2418034
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418034/range2418034/1
component: BBa_I0500

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type: Range
persistent identity: range2418034
display id: range2418034
version: 1
top level: BBa_K1486008
direction: direction/reverse
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418036
display id: annotation2418036
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418036/range2418036/1
component: BBa_B0034

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type: Range
persistent identity: range2418036
display id: range2418036
version: 1
top level: BBa_K1486008
direction: direction/reverse
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418040
display id: annotation2418040
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418040/range2418040/1
component: BBa_K1486000

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type: Range
persistent identity: range2418040
display id: range2418040
version: 1
top level: BBa_K1486008
direction: direction/reverse
start: 1223
end: 1924
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418041
display id: annotation2418041
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418041/range2418041/1
component: BBa_K1486004

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type: Range
persistent identity: range2418041
display id: range2418041
version: 1
top level: BBa_K1486008
direction: direction/reverse
start: 1925
end: 1954
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2418045
display id: annotation2418045
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418045/range2418045/1
component: BBa_K1486006

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type: Range
persistent identity: range2418045
display id: range2418045
version: 1
top level: BBa_K1486008
direction: direction/reverse
start: 1955
end: 2359
orientation: inline

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2418046
display id: annotation2418046
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418046/range2418046/1
component: BBa_K1486052

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type: Range
persistent identity: range2418046
display id: range2418046
version: 1
top level: BBa_K1486008
direction: direction/reverse
start: 2360
end: 2397
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418048
display id: annotation2418048
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418048/range2418048/1
component: BBa_B0034

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type: Range
persistent identity: range2418048
display id: range2418048
version: 1
top level: BBa_K1486008
direction: direction/reverse
start: 2398
end: 2409
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418052
display id: annotation2418052
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418052/range2418052/1
component: BBa_K1486000

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type: Range
persistent identity: range2418052
display id: range2418052
version: 1
top level: BBa_K1486008
direction: direction/reverse
start: 2410
end: 3111
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418053
display id: annotation2418053
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418053/range2418053/1
component: BBa_K1486004

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type: Range
persistent identity: range2418053
display id: range2418053
version: 1
top level: BBa_K1486008
direction: direction/reverse
start: 3112
end: 3141
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2418057
display id: annotation2418057
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418057/range2418057/1
component: BBa_K1486007

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type: Range
persistent identity: range2418057
display id: range2418057
version: 1
top level: BBa_K1486008
direction: direction/reverse
start: 3142
end: 3726
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

BBa_K1486000
type: ComponentDefinition
persistent identity: BBa K1486000
display id: BBa_K1486000
version: 1
was generated by: iGEM to SBOL conversion
description: CpxR coding region
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: The part has been extracted from the E coli genome.
owned by: james
owned by: myers
mutable description: CpxR is a transciption factor that dimerizes and becomes active when phosphorylated by the transmembrane histidine kinase CpxA. CpxA in turn becomes active when the bacterial cell is submitted to different kinds of shock (pH, temperature, pressure, protein misfolding in the inter-membrane space...).
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When making composite parts with CpxR that are supposed to dimerize, we had to take care about the positioning of the other elements regarding the parallel dimerization of CpxR.
star: false
creator: iGem EPFL 2014
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: START
sequence annotation: CpxR
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K1486000_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2382022
display id: annotation2382022
version: 1
top level: BBa_K1486000
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382022/range2382022/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382022/range2382022/1
type: Range
persistent identity: range2382022
display id: range2382022
version: 1
top level: BBa_K1486000
direction: direction/forward
start: 1
end: 3
orientation: inline

CpxR
type: SequenceAnnotation
persistent identity: annotation2382021
display id: annotation2382021
version: 1
top level: BBa_K1486000
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382021/range2382021/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382021/range2382021/1
type: Range
persistent identity: range2382021
display id: range2382021
version: 1
top level: BBa_K1486000
direction: direction/forward
start: 1
end: 702
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2382023
display id: annotation2382023
version: 1
top level: BBa_K1486000
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382023/range2382023/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382023/range2382023/1
type: Range
persistent identity: range2382023
display id: range2382023
version: 1
top level: BBa_K1486000
direction: direction/forward
start: 699
end: 702
orientation: inline

BBa_K1486004
type: ComponentDefinition
persistent identity: BBa K1486004
display id: BBa_K1486004
version: 1
was generated by: iGEM to SBOL conversion
description: flexible linker 2x (GGGGS)
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: Flexible linker used to fuse two proteins.
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Designed considering the flexibility and separation needed between our fused proteins.
star: false
creator: iGem EPFL 2014
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1486004_sequence/1

BBa_K1486006
type: ComponentDefinition
persistent identity: BBa K1486006
display id: BBa_K1486006
version: 1
was generated by: iGEM to SBOL conversion
description: IFP[1]
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
mutable description: This is the first part of the split infrared fluorescent protein (IFP1.4). To be used with IFP[2].
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21033
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When fused to another coding sequence, insert a flexible linker
star: false
creator: iGem EPFL 2014
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: IFP[1]
sequence annotation: START
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K1486006_sequence/1

IFP[1]
type: SequenceAnnotation
persistent identity: annotation2418010
display id: annotation2418010
version: 1
top level: BBa_K1486006
location: https://synbiohub.org/public/igem/BBa_K1486006/annotation2418010/range2418010/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1486006/annotation2418010/range2418010/1
type: Range
persistent identity: range2418010
display id: range2418010
version: 1
top level: BBa_K1486006
direction: direction/forward
start: 1
end: 405
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2418009
display id: annotation2418009
version: 1
top level: BBa_K1486006
location: https://synbiohub.org/public/igem/BBa_K1486006/annotation2418009/range2418009/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1486006/annotation2418009/range2418009/1
type: Range
persistent identity: range2418009
display id: range2418009
version: 1
top level: BBa_K1486006
direction: direction/forward
start: 1
end: 3
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2418011
display id: annotation2418011
version: 1
top level: BBa_K1486006
location: https://synbiohub.org/public/igem/BBa_K1486006/annotation2418011/range2418011/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1486006/annotation2418011/range2418011/1
type: Range
persistent identity: range2418011
display id: range2418011
version: 1
top level: BBa_K1486006
direction: direction/forward
start: 403
end: 405
orientation: inline

BBa_K1486007
type: ComponentDefinition
persistent identity: BBa K1486007
display id: BBa_K1486007
version: 1
was generated by: iGEM to SBOL conversion
description: IFP[2]
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
mutable description: This is the second part of the split infrared fluorescent protein (IFP1.4). To be used with IFP[1].
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When fused to another coding sequence, insert a flexible linker.
star: false
creator: iGem EPFL 2014
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: IFP[2]
sequence annotation: START
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K1486007_sequence/1

IFP[2]
type: SequenceAnnotation
persistent identity: annotation2382164
display id: annotation2382164
version: 1
top level: BBa_K1486007
location: https://synbiohub.org/public/igem/BBa_K1486007/annotation2382164/range2382164/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1486007/annotation2382164/range2382164/1
type: Range
persistent identity: range2382164
display id: range2382164
version: 1
top level: BBa_K1486007
direction: direction/forward
start: 1
end: 579
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2382128
display id: annotation2382128
version: 1
top level: BBa_K1486007
location: https://synbiohub.org/public/igem/BBa_K1486007/annotation2382128/range2382128/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1486007/annotation2382128/range2382128/1
type: Range
persistent identity: range2382128
display id: range2382128
version: 1
top level: BBa_K1486007
direction: direction/forward
start: 1
end: 3
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2382163
display id: annotation2382163
version: 1
top level: BBa_K1486007
location: https://synbiohub.org/public/igem/BBa_K1486007/annotation2382163/range2382163/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1486007/annotation2382163/range2382163/1
type: Range
persistent identity: range2382163
display id: range2382163
version: 1
top level: BBa_K1486007
direction: direction/forward
start: 577
end: 579
orientation: inline

BBa_K1486052
type: ComponentDefinition
persistent identity: BBa K1486052
display id: BBa_K1486052
version: 1
was generated by: iGEM to SBOL conversion
description: Spacer
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: to edit
owned by: james
owned by: myers
mutable description: to edit
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: to edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1486052_sequence/1

https://synbiohub.org/public/igem/BBa_K1486008_sequence/1
type: Sequence
persistent identity: BBa K1486008 sequence
display id: BBa_K1486008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcaaagaggagaaaatgaataaaatcctgttagttgatgatgaccgagagctgacttccctattaaaggagctgctcgagatggaaggcttcaacgtgattgttgcccacgatggggaacaggcgcttgatcttctggacgacagcattgatttacttttgcttgacgtaatgatgccgaagaaaaatggtatcgacacattaaaagcacttcgccagacacaccagacgcctgtcattatgttgacggcgcgcggcagtgaacttgatcgcgttctcggccttgagctgggcgcagatgactatctcccgaaaccgtttaatgatcgtgagctggtggcacgtattcgcgcgatcctgcgccgttcgcactggagcgagcaacagcaaaacaacgacaacggttcaccgacactggaagttgatgccttagtgctgaatccaggccgtcaggaagccagcttcgacgggcaaacgctggagttaaccggtactgagtttaccctgctctatttgctggcacagcatctgggtcaggtggtttcccgtgaacatttaagccaggaagtgttgggcaaacgcctgacgcctttcgaccgcgctattgatatgcacatttccaacctgcgtcgtaaactgccggatcgtaaagatggtcacccgtggtttaaaaccttgcgtggtcgcggctatctgatggtttctgcttcataataaggtggtggtggttctggtggtggtggttctatgtccggagctcgggaccctctgccattctttccacctctgtacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486000_sequence/1
type: Sequence
persistent identity: BBa K1486000 sequence
display id: BBa_K1486000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaataaaatcctgttagttgatgatgaccgagagctgacttccctattaaaggagctgctcgagatggaaggcttcaacgtgattgttgcccacgatggggaacaggcgcttgatcttctggacgacagcattgatttacttttgcttgacgtaatgatgccgaagaaaaatggtatcgacacattaaaagcacttcgccagacacaccagacgcctgtcattatgttgacggcgcgcggcagtgaacttgatcgcgttctcggccttgagctgggcgcagatgactatctcccgaaaccgtttaatgatcgtgagctggtggcacgtattcgcgcgatcctgcgccgttcgcactggagcgagcaacagcaaaacaacgacaacggttcaccgacactggaagttgatgccttagtgctgaatccaggccgtcaggaagccagcttcgacgggcaaacgctggagttaaccggtactgagtttaccctgctctatttgctggcacagcatctgggtcaggtggtttcccgtgaacatttaagccaggaagtgttgggcaaacgcctgacgcctttcgaccgcgctattgatatgcacatttccaacctgcgtcgtaaactgccggatcgtaaagatggtcacccgtggtttaaaaccttgcgtggtcgcggctatctgatggtttctgcttcataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486004_sequence/1
type: Sequence
persistent identity: BBa K1486004 sequence
display id: BBa_K1486004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggtggtggttctggtggtggtggttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486006_sequence/1
type: Sequence
persistent identity: BBa K1486006 sequence
display id: BBa_K1486006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccggagctcgggaccctctgccattctttccacctctgtacctgggcggccctgagattacaaccgagaactgcgagagagagcctatccacattcctgggtccatccagccacacggggctctgctcacagctgacggccactccggagaggtgctccaagtgtccctgaatgccgctaccttcctgggccaggagcctactgtgctgcgggggcagaccctggctgccctgctccccgagcagtggccagccctgcaggcagccctgcccccaggatgtccagatgccctccaatacagggccaccctcgactggccagctgctgggcacctcagcctgactgtgcatcgggtggctgaactcctgatcctggagttcgaacctaccgaggcctggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486007_sequence/1
type: Sequence
persistent identity: BBa K1486007 sequence
display id: BBa_K1486007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccggagctcgggacagcattggccctcacgccctgaggaacgccatgtttgctctggaaagcgctccaaacctgcgggctctggccgaagtcgcaacacaaactgtgagagaactgactggcttcgatcgggtgatgctgtacaaatttgcccctgacgccactggagagatgattgctgaggccagacgggagggcatgcaggcttttctgggccacaggtttcccgcatcccacacccctgcacaagctagggccctctacacaagacacctgctccggctgaccgcagacaccagggctgcagcagtgcccctcgaccccgtgctgaatccccagacaaatgctcctacacctctgggcggagctgtcctcagagctacatccccaatgcacatgcagtacctgaggaatatgggagtgggctcctccctgagcgtcagcgtcgtggtcggcggccagctgtggggactgattgtctgccaccatcagacaccctacgtgctgccaccagatctgcggaccaccctggaggagctggggagaaagctgtccgggcaggtgcagaggaaagaagcctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486052_sequence/1
type: Sequence
persistent identity: BBa K1486052 sequence
display id: BBa_K1486052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatgataaacgaaagcgtcatcccgggtgtagtagta
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z