BBa_K1486007 1 BBa_K1486007 IFP[2] 2014-08-24T11:00:00Z 2015-05-08T01:10:42Z From Michnick Article. To Edit This is the second part of the split infrared fluorescent protein (IFP1.4). To be used with IFP[1]. false false _1866_ 0 21042 9 Not in stock false When fused to another coding sequence, insert a flexible linker. false iGem EPFL 2014 annotation2382128 1 START range2382128 1 1 3 annotation2382163 1 STOP range2382163 1 577 579 annotation2382164 1 IFP[2] range2382164 1 1 579 BBa_K1486011 1 BBa_K1486011 CxpR & Split IFP1.4 [Cterm + Nterm] 2014-08-26T11:00:00Z 2015-05-08T01:10:42Z From Michnick Article. To Edit This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008)<BR> - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010)<BR> - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)<BR> false false _1866_ 0 21042 9 It's complicated false All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them. false iGem EPFL 2014 component2382237 1 BBa_B0034 component2382249 1 BBa_K1486007 component2382241 1 BBa_K1486000 component2382242 1 BBa_K1486004 component2382235 1 BBa_I0500 component2382243 1 BBa_K1486006 component2382250 1 BBa_K1486004 component2382245 1 BBa_B0034 component2382254 1 BBa_K1486000 annotation2382249 1 BBa_K1486007 range2382249 1 2549 3127 annotation2382243 1 BBa_K1486006 range2382243 1 1955 2536 annotation2382250 1 BBa_K1486004 range2382250 1 3128 3157 annotation2382237 1 BBa_B0034 range2382237 1 1211 1222 annotation2382241 1 BBa_K1486000 range2382241 1 1223 1924 annotation2382245 1 BBa_B0034 range2382245 1 2537 2548 annotation2382254 1 BBa_K1486000 range2382254 1 3158 3859 annotation2382242 1 BBa_K1486004 range2382242 1 1925 1954 annotation2382235 1 BBa_I0500 range2382235 1 1 1210 BBa_K1486000 1 BBa_K1486000 CpxR coding region 2014-08-11T11:00:00Z 2015-05-08T01:10:42Z The part has been extracted from the E coli genome. CpxR is a transciption factor that dimerizes and becomes active when phosphorylated by the transmembrane histidine kinase CpxA. CpxA in turn becomes active when the bacterial cell is submitted to different kinds of shock (pH, temperature, pressure, protein misfolding in the inter-membrane space...). false false _1866_ 0 21042 9 Not in stock false When making composite parts with CpxR that are supposed to dimerize, we had to take care about the positioning of the other elements regarding the parallel dimerization of CpxR. false iGem EPFL 2014 annotation2382021 1 CpxR range2382021 1 1 702 annotation2382022 1 START range2382022 1 1 3 annotation2382023 1 STOP range2382023 1 699 702 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1486004 1 BBa_K1486004 flexible linker 2x (GGGGS) 2014-08-11T11:00:00Z 2015-05-08T01:10:42Z Synthetic. Flexible linker used to fuse two proteins. false false _1866_ 0 21042 9 Not in stock false Designed considering the flexibility and separation needed between our fused proteins. false iGem EPFL 2014 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498843 1 AraC range1498843 1 1 1079 annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498842 1 PC range1498842 1 1043 1114 BBa_K1486006 1 BBa_K1486006 IFP[1] 2014-08-24T11:00:00Z 2015-05-08T01:10:42Z From Michnick Article. To Edit This is the first part of the split infrared fluorescent protein (IFP1.4). To be used with IFP[2]. false false _1866_ 0 21033 9 Not in stock false When fused to another coding sequence, insert a flexible linker false iGem EPFL 2014 annotation2418009 1 START range2418009 1 1 3 annotation2418010 1 IFP[1] range2418010 1 1 405 annotation2418011 1 STOP range2418011 1 403 405 BBa_K1486011_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcaaagaggagaaaatgaataaaatcctgttagttgatgatgaccgagagctgacttccctattaaaggagctgctcgagatggaaggcttcaacgtgattgttgcccacgatggggaacaggcgcttgatcttctggacgacagcattgatttacttttgcttgacgtaatgatgccgaagaaaaatggtatcgacacattaaaagcacttcgccagacacaccagacgcctgtcattatgttgacggcgcgcggcagtgaacttgatcgcgttctcggccttgagctgggcgcagatgactatctcccgaaaccgtttaatgatcgtgagctggtggcacgtattcgcgcgatcctgcgccgttcgcactggagcgagcaacagcaaaacaacgacaacggttcaccgacactggaagttgatgccttagtgctgaatccaggccgtcaggaagccagcttcgacgggcaaacgctggagttaaccggtactgagtttaccctgctctatttgctggcacagcatctgggtcaggtggtttcccgtgaacatttaagccaggaagtgttgggcaaacgcctgacgcctttcgaccgcgctattgatatgcacatttccaacctgcgtcgtaaactgccggatcgtaaagatggtcacccgtggtttaaaaccttgcgtggtcgcggctatctgatggtttctgcttcataataaggtggtggtggttctggtggtggtggttcttccggagctcgggacagcattggccctcacgccctgaggaacgccatgtttgctctggaaagcgctccaaacctgcgggctctggccgaagtcgcaacacaaactgtgagagaactgactggcttcgatcgggtgatgctgtacaaatttgcccctgacgccactggagagatgattgctgaggccagacgggagggcatgcaggcttttctgggccacaggtttcccgcatcccacacccctgcacaagctagggccctctacacaagacacctgctccggctgaccgcagacaccagggctgcagcagtgcccctcgaccccgtgctgaatccccagacaaatgctcctacacctctgggcggagctgtcctcagagctacatccccaatgcacatgcagtacctgaggaatatgggagtgggctcctccctgagcgtcagcgtcgtggtcggcggccagctgtggggactgattgtctgccaccatcagacaccctacgtgctgccaccagatctgcggaccaccctggaggagctggggagaaagctgtccgggcaggtgcagaggaaagaagcctaaaaagaggagaaaatggctcgggacagcattggccctcacgccctgaggaacgccatgtttgctctggaaagcgctccaaacctgcgggctctggccgaagtcgcaacacaaactgtgagagaactgactggcttcgatcgggtgatgctgtacaaatttgcccctgacgccactggagagatgattgctgaggccagacgggagggcatgcaggcttttctgggccacaggtttcccgcatcccacacccctgcacaagctagggccctctacacaagacacctgctccggctgaccgcagacaccagggctgcagcagtgcccctcgaccccgtgctgaatccccagacaaatgctcctacacctctgggcggagctgtcctcagagctacatccccaatgcacatgcagtacctgaggaatatgggagtgggctcctccctgagcgtcagcgtcgtggtcggcggccagctgtggggactgattgtctgccaccatcagacaccctacgtgctgccaccagatctgcggaccaccctggaggagctggggagaaagctgtccgggcaggtgcagaggaaagaagcctaaggtggtggtggttctggtggtggtggttctatgaataaaatcctgttagttgatgatgaccgagagctgacttccctattaaaggagctgctcgagatggaaggcttcaacgtgattgttgcccacgatggggaacaggcgcttgatcttctggacgacagcattgatttacttttgcttgacgtaatgatgccgaagaaaaatggtatcgacacattaaaagcacttcgccagacacaccagacgcctgtcattatgttgacggcgcgcggcagtgaacttgatcgcgttctcggccttgagctgggcgcagatgactatctcccgaaaccgtttaatgatcgtgagctggtggcacgtattcgcgcgatcctgcgccgttcgcactggagcgagcaacagcaaaacaacgacaacggttcaccgacactggaagttgatgccttagtgctgaatccaggccgtcaggaagccagcttcgacgggcaaacgctggagttaaccggtactgagtttaccctgctctatttgctggcacagcatctgggtcaggtggtttcccgtgaacatttaagccaggaagtgttgggcaaacgcctgacgcctttcgaccgcgctattgatatgcacatttccaacctgcgtcgtaaactgccggatcgtaaagatggtcacccgtggtttaaaaccttgcgtggtcgcggctatctgatggtttctgcttcataataa BBa_K1486006_sequence 1 atgtccggagctcgggaccctctgccattctttccacctctgtacctgggcggccctgagattacaaccgagaactgcgagagagagcctatccacattcctgggtccatccagccacacggggctctgctcacagctgacggccactccggagaggtgctccaagtgtccctgaatgccgctaccttcctgggccaggagcctactgtgctgcgggggcagaccctggctgccctgctccccgagcagtggccagccctgcaggcagccctgcccccaggatgtccagatgccctccaatacagggccaccctcgactggccagctgctgggcacctcagcctgactgtgcatcgggtggctgaactcctgatcctggagttcgaacctaccgaggcctggtaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1486007_sequence 1 atgtccggagctcgggacagcattggccctcacgccctgaggaacgccatgtttgctctggaaagcgctccaaacctgcgggctctggccgaagtcgcaacacaaactgtgagagaactgactggcttcgatcgggtgatgctgtacaaatttgcccctgacgccactggagagatgattgctgaggccagacgggagggcatgcaggcttttctgggccacaggtttcccgcatcccacacccctgcacaagctagggccctctacacaagacacctgctccggctgaccgcagacaccagggctgcagcagtgcccctcgaccccgtgctgaatccccagacaaatgctcctacacctctgggcggagctgtcctcagagctacatccccaatgcacatgcagtacctgaggaatatgggagtgggctcctccctgagcgtcagcgtcgtggtcggcggccagctgtggggactgattgtctgccaccatcagacaccctacgtgctgccaccagatctgcggaccaccctggaggagctggggagaaagctgtccgggcaggtgcagaggaaagaagcctaa BBa_K1486000_sequence 1 atgaataaaatcctgttagttgatgatgaccgagagctgacttccctattaaaggagctgctcgagatggaaggcttcaacgtgattgttgcccacgatggggaacaggcgcttgatcttctggacgacagcattgatttacttttgcttgacgtaatgatgccgaagaaaaatggtatcgacacattaaaagcacttcgccagacacaccagacgcctgtcattatgttgacggcgcgcggcagtgaacttgatcgcgttctcggccttgagctgggcgcagatgactatctcccgaaaccgtttaatgatcgtgagctggtggcacgtattcgcgcgatcctgcgccgttcgcactggagcgagcaacagcaaaacaacgacaacggttcaccgacactggaagttgatgccttagtgctgaatccaggccgtcaggaagccagcttcgacgggcaaacgctggagttaaccggtactgagtttaccctgctctatttgctggcacagcatctgggtcaggtggtttcccgtgaacatttaagccaggaagtgttgggcaaacgcctgacgcctttcgaccgcgctattgatatgcacatttccaacctgcgtcgtaaactgccggatcgtaaagatggtcacccgtggtttaaaaccttgcgtggtcgcggctatctgatggtttctgcttcataataa BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_K1486004_sequence 1 ggtggtggtggttctggtggtggtggttct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z