BBa_K1486000 1 BBa_K1486000 CpxR coding region 2014-08-11T11:00:00Z 2015-05-08T01:10:42Z The part has been extracted from the E coli genome. CpxR is a transciption factor that dimerizes and becomes active when phosphorylated by the transmembrane histidine kinase CpxA. CpxA in turn becomes active when the bacterial cell is submitted to different kinds of shock (pH, temperature, pressure, protein misfolding in the inter-membrane space...). false false _1866_ 0 21042 9 Not in stock false When making composite parts with CpxR that are supposed to dimerize, we had to take care about the positioning of the other elements regarding the parallel dimerization of CpxR. false iGem EPFL 2014 annotation2382023 1 STOP range2382023 1 699 702 annotation2382021 1 CpxR range2382021 1 1 702 annotation2382022 1 START range2382022 1 1 3 BBa_K1486004 1 BBa_K1486004 flexible linker 2x (GGGGS) 2014-08-11T11:00:00Z 2015-05-08T01:10:42Z Synthetic. Flexible linker used to fuse two proteins. false false _1866_ 0 21042 9 Not in stock false Designed considering the flexibility and separation needed between our fused proteins. false iGem EPFL 2014 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498842 1 PC range1498842 1 1043 1114 annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498843 1 AraC range1498843 1 1 1079 BBa_K1486006 1 BBa_K1486006 IFP[1] 2014-08-24T11:00:00Z 2015-05-08T01:10:42Z From Michnick Article. To Edit This is the first part of the split infrared fluorescent protein (IFP1.4). To be used with IFP[2]. false false _1866_ 0 21033 9 Not in stock false When fused to another coding sequence, insert a flexible linker false iGem EPFL 2014 annotation2418009 1 START range2418009 1 1 3 annotation2418011 1 STOP range2418011 1 403 405 annotation2418010 1 IFP[1] range2418010 1 1 405 BBa_K1486012 1 BBa_K1486012 CpxR-IFP[1] under pBAD 2014-08-31T11:00:00Z 2015-05-08T01:10:42Z To Edit This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[1]. Biobrick BBa_K1486013 has CpxR-IFP[2] with an arabinose promoter. This construct was made in both orientations: BBa_K1486014 is IFP[1]-CpxR (with an arabinose promoter). false false _1866_ 0 21042 9 It's complicated false Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them. false iGem EPFL 2014 component2382293 1 BBa_K1486006 component2382292 1 BBa_K1486004 component2382287 1 BBa_B0034 component2382291 1 BBa_K1486000 component2382285 1 BBa_I0500 annotation2382285 1 BBa_I0500 range2382285 1 1 1210 annotation2382292 1 BBa_K1486004 range2382292 1 1925 1954 annotation2382291 1 BBa_K1486000 range2382291 1 1223 1924 annotation2382287 1 BBa_B0034 range2382287 1 1211 1222 annotation2382293 1 BBa_K1486006 range2382293 1 1955 2536 BBa_K1486006_sequence 1 atgtccggagctcgggaccctctgccattctttccacctctgtacctgggcggccctgagattacaaccgagaactgcgagagagagcctatccacattcctgggtccatccagccacacggggctctgctcacagctgacggccactccggagaggtgctccaagtgtccctgaatgccgctaccttcctgggccaggagcctactgtgctgcgggggcagaccctggctgccctgctccccgagcagtggccagccctgcaggcagccctgcccccaggatgtccagatgccctccaatacagggccaccctcgactggccagctgctgggcacctcagcctgactgtgcatcgggtggctgaactcctgatcctggagttcgaacctaccgaggcctggtaa BBa_K1486012_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcaaagaggagaaaatgaataaaatcctgttagttgatgatgaccgagagctgacttccctattaaaggagctgctcgagatggaaggcttcaacgtgattgttgcccacgatggggaacaggcgcttgatcttctggacgacagcattgatttacttttgcttgacgtaatgatgccgaagaaaaatggtatcgacacattaaaagcacttcgccagacacaccagacgcctgtcattatgttgacggcgcgcggcagtgaacttgatcgcgttctcggccttgagctgggcgcagatgactatctcccgaaaccgtttaatgatcgtgagctggtggcacgtattcgcgcgatcctgcgccgttcgcactggagcgagcaacagcaaaacaacgacaacggttcaccgacactggaagttgatgccttagtgctgaatccaggccgtcaggaagccagcttcgacgggcaaacgctggagttaaccggtactgagtttaccctgctctatttgctggcacagcatctgggtcaggtggtttcccgtgaacatttaagccaggaagtgttgggcaaacgcctgacgcctttcgaccgcgctattgatatgcacatttccaacctgcgtcgtaaactgccggatcgtaaagatggtcacccgtggtttaaaaccttgcgtggtcgcggctatctgatggtttctgcttcataataaggtggtggtggttctggtggtggtggttcttccggagctcgggacagcattggccctcacgccctgaggaacgccatgtttgctctggaaagcgctccaaacctgcgggctctggccgaagtcgcaacacaaactgtgagagaactgactggcttcgatcgggtgatgctgtacaaatttgcccctgacgccactggagagatgattgctgaggccagacgggagggcatgcaggcttttctgggccacaggtttcccgcatcccacacccctgcacaagctagggccctctacacaagacacctgctccggctgaccgcagacaccagggctgcagcagtgcccctcgaccccgtgctgaatccccagacaaatgctcctacacctctgggcggagctgtcctcagagctacatccccaatgcacatgcagtacctgaggaatatgggagtgggctcctccctgagcgtcagcgtcgtggtcggcggccagctgtggggactgattgtctgccaccatcagacaccctacgtgctgccaccagatctgcggaccaccctggaggagctggggagaaagctgtccgggcaggtgcagaggaaagaagcctaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1486000_sequence 1 atgaataaaatcctgttagttgatgatgaccgagagctgacttccctattaaaggagctgctcgagatggaaggcttcaacgtgattgttgcccacgatggggaacaggcgcttgatcttctggacgacagcattgatttacttttgcttgacgtaatgatgccgaagaaaaatggtatcgacacattaaaagcacttcgccagacacaccagacgcctgtcattatgttgacggcgcgcggcagtgaacttgatcgcgttctcggccttgagctgggcgcagatgactatctcccgaaaccgtttaatgatcgtgagctggtggcacgtattcgcgcgatcctgcgccgttcgcactggagcgagcaacagcaaaacaacgacaacggttcaccgacactggaagttgatgccttagtgctgaatccaggccgtcaggaagccagcttcgacgggcaaacgctggagttaaccggtactgagtttaccctgctctatttgctggcacagcatctgggtcaggtggtttcccgtgaacatttaagccaggaagtgttgggcaaacgcctgacgcctttcgaccgcgctattgatatgcacatttccaacctgcgtcgtaaactgccggatcgtaaagatggtcacccgtggtttaaaaccttgcgtggtcgcggctatctgatggtttctgcttcataataa BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_K1486004_sequence 1 ggtggtggtggttctggtggtggtggttct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z