BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498843 1 AraC range1498843 1 1 1079 annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498842 1 PC range1498842 1 1043 1114 BBa_K1486017 1 BBa_K1486017 fLuc[2] 2014-09-14T11:00:00Z 2015-05-08T01:10:42Z to edit This is the second part of the split Firefly luciferase. To be used with fLuc[1]. false false _1866_ 0 21042 9 Not in stock false to edit false EPFL iGem team 2014 BBa_K1486018 1 BBa_K1486018 Arabinose promoter + fLuc[1] + fLuc[2] 2014-09-14T11:00:00Z 2015-05-08T01:10:42Z to edit This part contains both fragments of the split firefly luciferase, each of them preceded by an Elowitz RBS. false false _1866_ 0 21042 9 It's complicated false to edit (start/stop codons) false EPFL iGem team 2014 component2383441 1 BBa_I0500 component2383444 1 BBa_K1486016 component2383447 1 BBa_K1486017 component2383446 1 BBa_B0034 component2383443 1 BBa_B0034 annotation2383441 1 BBa_I0500 range2383441 1 1 1210 annotation2383446 1 BBa_B0034 range2383446 1 1697 1708 annotation2383444 1 BBa_K1486016 range2383444 1 1223 1696 annotation2383447 1 BBa_K1486017 range2383447 1 1709 2956 annotation2383443 1 BBa_B0034 range2383443 1 1211 1222 BBa_K1486016 1 BBa_K1486016 fLuc[1] 2014-09-14T11:00:00Z 2015-05-08T01:10:42Z to edit This is the first part of the split Firefly luciferase. To be used with fLuc[2]. false false _1866_ 0 21042 9 Not in stock false to edit false EPFL iGem team 2014 BBa_B0034_sequence 1 aaagaggagaaa BBa_K1486017_sequence 1 atggaggacgcgaaaaacatcaagaaaggcccagcaccgttctatccgctggaagatggtaccgccggtgaacaattgcacaaagcgatgaaacgttatgcgctggttccgggtaccatcgcattcacggacgcgcatatcgaggttaacatcacctatgccgagtacttcgagatgagcgttcgtctggccgaggcaatgaagcgttatggtttgaataccaatcatcgcatcgtcgtctgtagcgagaacagcttgcagtttttcatgccagttctgggtgcactgtttattggtgtggcagtggcgcctgcgaatgatatctacaacgagcgtgaactgttgaatagcatgaacatttcgcagccgacggttgtttttgtgtccaagaaaggtttgcagaagattctgaatgtgcaaaagaagttgccgatcattcagaaaatcatcatcatggacagcaagaccgattaccagggtttccaaagcatgtacacttttgtgacgagccacctgcctccgggttttaacgagtatgatttcgttccggaaagcttcgaccgtgataagacgattgcactgattatgaactcgtccggctctaccggcctgcctaaaggcgtggccctgccgcaccgtactgcatgtgtccgcttcagccacgcgcgtgatccgatcttcggtaaccaaatcattccggacaccgccattttgtctgtcgttccgtttcatcatggctttggtatgtttaccactctgggttacctgatctgtggtttccgtgttgttctgatgtaccgtttcgaagaagaactgttcctgcgtagcctgcaagattacaagatccaatccgcgctgctggtgccgacgctgtttagcttcttcgctaaatccaccctgattgacaaatatgacttgagcaacctgcacgaaatcgcgtcgggcggtgcgccgctgagcaaagaagtcggtgaggccgttgcgaaacgtttccatctgccgggcatccgtcagggttatggcctgacggaaacgaccagcgcgattttgattacccctgagggtgacgataaaccgggtgccgttggcaaggtggtaccgttctttgaggctaaagtcgtggacctggacacgggtaagactctgggtgtgaatcaacgtggcgagttgtgcgttcgtggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggc BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_K1486018_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcaaagaggagaaaggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggctggctgcacagcggcgacctggcgtactgggatgaggatgaacactttttcatcgttggtcgcctgaagagcctgatcaagtacaagggctaccaggtggcaccggctgaactggagagcattctgttgcaacaccctaatatcttcgacgcgggtgttgcgggtctgcctgatgatgatgccggtgaattgccagctgcggtggtcgttctggaacacggtaaaacgatgactgaaaaagaaatcgtggactacgtcgcctcgcaggtgaccaccgctaaaaagttgcgtggtggcgtggtgtttgtggatgaagtgccgaaaggcctgacgggtaaacgtgatgcacgtaagattcgtgagatcttgatcaaggcgaagaaaggtggtaaatccaagttgtaaaaagaggagaaaatggaggacgcgaaaaacatcaagaaaggcccagcaccgttctatccgctggaagatggtaccgccggtgaacaattgcacaaagcgatgaaacgttatgcgctggttccgggtaccatcgcattcacggacgcgcatatcgaggttaacatcacctatgccgagtacttcgagatgagcgttcgtctggccgaggcaatgaagcgttatggtttgaataccaatcatcgcatcgtcgtctgtagcgagaacagcttgcagtttttcatgccagttctgggtgcactgtttattggtgtggcagtggcgcctgcgaatgatatctacaacgagcgtgaactgttgaatagcatgaacatttcgcagccgacggttgtttttgtgtccaagaaaggtttgcagaagattctgaatgtgcaaaagaagttgccgatcattcagaaaatcatcatcatggacagcaagaccgattaccagggtttccaaagcatgtacacttttgtgacgagccacctgcctccgggttttaacgagtatgatttcgttccggaaagcttcgaccgtgataagacgattgcactgattatgaactcgtccggctctaccggcctgcctaaaggcgtggccctgccgcaccgtactgcatgtgtccgcttcagccacgcgcgtgatccgatcttcggtaaccaaatcattccggacaccgccattttgtctgtcgttccgtttcatcatggctttggtatgtttaccactctgggttacctgatctgtggtttccgtgttgttctgatgtaccgtttcgaagaagaactgttcctgcgtagcctgcaagattacaagatccaatccgcgctgctggtgccgacgctgtttagcttcttcgctaaatccaccctgattgacaaatatgacttgagcaacctgcacgaaatcgcgtcgggcggtgcgccgctgagcaaagaagtcggtgaggccgttgcgaaacgtttccatctgccgggcatccgtcagggttatggcctgacggaaacgaccagcgcgattttgattacccctgagggtgacgataaaccgggtgccgttggcaaggtggtaccgttctttgaggctaaagtcgtggacctggacacgggtaagactctgggtgtgaatcaacgtggcgagttgtgcgttcgtggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggc BBa_K1486016_sequence 1 ggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggctggctgcacagcggcgacctggcgtactgggatgaggatgaacactttttcatcgttggtcgcctgaagagcctgatcaagtacaagggctaccaggtggcaccggctgaactggagagcattctgttgcaacaccctaatatcttcgacgcgggtgttgcgggtctgcctgatgatgatgccggtgaattgccagctgcggtggtcgttctggaacacggtaaaacgatgactgaaaaagaaatcgtggactacgtcgcctcgcaggtgaccaccgctaaaaagttgcgtggtggcgtggtgtttgtggatgaagtgccgaaaggcctgacgggtaaacgtgatgcacgtaagattcgtgagatcttgatcaaggcgaagaaaggtggtaaatccaagttgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z