LOCUS BBa_K1486026 2535 bp DNA linear 29-Nov-2024 DEFINITION sfGFP + Kanamycin resistance for yeast ACCESSION BBa_K1486026 VERSION BBa_K1486026.1 FEATURES Location/Qualifiers misc_feature 1..50 /label=Pbs2 flanking CDS 51..74 /label=8 a.a linker misc_feature 75..791 /label=BBa_K1486023 misc_feature 811..999 /label=Adh1 terminator misc_feature 1073..2430 /label=BBa_K1486024 misc_feature 2485..2535 /label=Pbs2 flanking ORIGIN 1 gtgagaatgg tttatctaaa aatgtaccgg cattacatat gggtggttta ggtgacggtg 61 ctggtttaat taacatgtct aaaggcgagg aattgtttac aggtgtagtt cctattttgg 121 tcgaactaga tggagatgtc aacggtcata agttttctgt acgtggtgaa ggcgaaggtg 181 atgctactaa tggcaaactg actctgaagt tcatctgtac gaccggaaaa ttgccagttc 241 catggcctac attagtaaca acattaacct acggagttca atgcttttca agatacccag 301 atcacatgaa gcagcatgac tttttcaaaa gtgctatgcc agaaggttat gtgcaggaga 361 gaactatctc cttcaaagat gatggtactt ataagacaag agcagaggta aagtttgaag 421 gcgatacatt ggttaataga attgagctta aaggcattga ttttaaggaa gatgggaata 481 tcctcggaca taaacttgaa tacaatttca attcacataa tgtttatatc acagcagata 541 aacaaaagaa tggtataaaa gcgaacttta agattagaca taacgtggaa gatggtagcg 601 tccaattagc cgaccattac caacaaaaca ctcctatcgg ggacgggcca gttctccttc 661 cagacaacca ctacctatca acccaatctg ttctatctaa agaccctaat gaaaaacgag 721 atcacatggt cttgttagag ttcgtgactg ccgctggtat aacattagga atggacgaat 781 tgtacaaata aggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat 841 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt 901 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt 961 atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag 1021 ggtaatatag atctgtttag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg 1081 aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact 1141 gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc atacattttg 1201 atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc gagcagggaa 1261 acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa 1321 aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa atcttgctag 1381 gatacagttc tcacatcaca tccgaacata aacaaccatg ggtaaggaaa agactcacgt 1441 ttcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg 1501 cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc 1561 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt 1621 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac 1681 tcctgatgat gcatggttac tcaccactgc gatccccggc aaaacagcat tccaggtatt 1741 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg 1801 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc 1861 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg 1921 taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc cattctcacc 1981 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa 2041 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc 2101 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa 2161 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt 2221 tttctaatca gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag 2281 tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt 2341 cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc 2401 aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca 2461 gtttaaacga gctcgaattc atcgacgtta atatccaaat aaaagcaaac aggcacgtga 2521 atataacaac aaaaa //