LOCUS       BBa_K1486029            2226 bp    DNA     linear       10-May-2024
DEFINITION  Yeast Optimized sfGFP-N + ADH1 terminator + Kanamycin resistance
ACCESSION   BBa_K1486029
VERSION     BBa_K1486029.1  
FEATURES             Location/Qualifiers
     misc_feature    1..39
                     /label=13 amino acids linker
     misc_feature    1..39
                     /label=BBa_K1486037
     CDS             40..681
                     /label=yeast optimized sfGFP N-terminus
     misc_feature    40..681
                     /label=BBa_K1486028
     misc_feature    682..869
                     /label=Adh1 terminator
     terminator      682..869
                     /label=BBa_K1486025
     promoter        870..1213
                     /label=TEF promoter
     CDS             1214..2023
                     /label=KanR
     misc_feature    2028..2226
                     /label=TEF terminator
     misc_feature    870..2226
                     /label=BBa_K1486024
ORIGIN
        1 ggcggtggct ctggaggtgg tgggtccggc ggtggctcta tgtctaaagg cgaggaattg
       61 tttacaggtg tagttcctat tttggtcgaa ctagatggag atgtcaacgg tcataagttt
      121 tctgtacgtg gtgaaggcga aggtgatgct actaatggca aactgactct gaagttcatc
      181 tgtacgaccg gaaaattgcc agttccatgg cctacattag taacaacatt aacctacgga
      241 gttcaatgct tttcaagata cccagatcac atgaagcagc atgacttttt caaaagtgct
      301 atgccagaag gttatgtgca ggagagaact atctccttca aagatgatgg tacttataag
      361 acaagagcag aggtaaagtt tgaaggcgat acattggtta atagaattga gcttaaaggc
      421 attgatttta aggaagatgg gaatatcctc ggacataaac ttgaatacaa tttcaattca
      481 cataatgttt atatcacagc agataaacaa aagaatggta taaaagcgaa ctttaagatt
      541 agacataacg tggaagatgg tagcgtccaa ttagccgacc attaccaaca aaacactcct
      601 atcggggacg ggccagttct ccttccagac aaccactacc tatcaaccca atctgttcta
      661 tctaaagacc ctaatgaaaa agcgaatttc ttatgattta tgatttttat tattaaataa
      721 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
      781 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag
      841 gtcgctctta ttgaccacac ctctaccggg acatggaggc ccagaatacc ctccttgaca
      901 gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac
      961 atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa
     1021 attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga
     1081 attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt
     1141 tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg
     1201 aacataaaca accatgggta aggaaaagac tcacgtttcg aggccgcgat taaattccaa
     1261 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     1321 gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     1381 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     1441 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     1501 cactgcgatc cccggcaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     1561 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     1621 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     1681 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     1741 ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     1801 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     1861 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     1921 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     1981 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagtac tgacaataaa
     2041 aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta
     2101 ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat
     2161 gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct
     2221 atactg
//