##gff-version 3 ##sequence-region BBa_K1486029 1 2226 BBa_K1486029 . engineered_region 870 2226 . + 0 ID=BBa_K1486024;Name=BBa_K1486024 BBa_K1486029 . stop_codon 2028 2226 . + 0 ID=annotation2416944;Name=TEF terminator;Parent=BBa_K1486024 BBa_K1486029 . promoter 870 1213 . + 0 ID=annotation2416942;Name=TEF promoter;Parent=BBa_K1486024 BBa_K1486029 . CDS 1214 2023 . + 0 ID=annotation2416943;Name=KanR;Parent=BBa_K1486024 BBa_K1486029 . terminator 682 869 . + 0 ID=BBa_K1486025;Name=BBa_K1486025 BBa_K1486029 . stop_codon 682 869 . + 0 ID=annotation2418776;Name=Adh1 terminator;Parent=BBa_K1486025 BBa_K1486029 . polypeptide_domain 1 39 . + 0 ID=BBa_K1486037;Name=BBa_K1486037 BBa_K1486029 . tag 1 39 . + 0 ID=annotation2417686;Name=13 amino acids linker;Parent=BBa_K1486037 BBa_K1486029 . tag 40 681 . + 0 ID=BBa_K1486028;Name=BBa_K1486028 BBa_K1486029 . CDS 40 681 . + 0 ID=annotation2417081;Name=yeast optimized sfGFP N-terminus;Parent=BBa_K1486028 >BBa_K1486029 ggcggtggctctggaggtggtgggtccggcggtggctctatgtctaaaggcgaggaattgtttacaggtgtagttccta ttttggtcgaactagatggagatgtcaacggtcataagttttctgtacgtggtgaaggcgaaggtgatgctactaatgg caaactgactctgaagttcatctgtacgaccggaaaattgccagttccatggcctacattagtaacaacattaacctac ggagttcaatgcttttcaagatacccagatcacatgaagcagcatgactttttcaaaagtgctatgccagaaggttatg tgcaggagagaactatctccttcaaagatgatggtacttataagacaagagcagaggtaaagtttgaaggcgatacatt ggttaatagaattgagcttaaaggcattgattttaaggaagatgggaatatcctcggacataaacttgaatacaatttc aattcacataatgtttatatcacagcagataaacaaaagaatggtataaaagcgaactttaagattagacataacgtgg aagatggtagcgtccaattagccgaccattaccaacaaaacactcctatcggggacgggccagttctccttccagacaa ccactacctatcaacccaatctgttctatctaaagaccctaatgaaaaagcgaatttcttatgatttatgatttttatt attaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattct tgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgg gacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtaca tttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacgg ctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtag agaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagtt ctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatg gatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatggga agcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagact aaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcacc actgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctgg cagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctca ggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaa gtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataacc ttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgc catcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcct gatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgtttt caagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttt tttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgc tggtcgctatactg