LOCUS       BBa_K1486032            2544 bp    DNA     linear       22-Nov-2024
DEFINITION  sfGFP + ADH1 terminator + Ura3 cassette
ACCESSION   BBa_K1486032
VERSION     BBa_K1486032.1  
FEATURES             Location/Qualifiers
     misc_feature    1..47
                     /label=Hog1 5' flanking region
     CDS             48..71
                     /label=8 a.a linker
     misc_feature    72..804
                     /label=BBa_K1486023
     misc_feature    808..996
                     /label=ADH1 terminator
     misc_feature    1070..2430
                     /label=BBa_K1486031
     misc_feature    2494..2544
                     /label=Hog1 3' flanking region
ORIGIN
        1 cggactacgg taaccaggcc atacagtacg ctaatgagtt ccaacagggt gacggtgctg
       61 gtttaattaa catgtctaaa ggcgaggaat tgtttacagg tgtagttcct attttggtcg
      121 aactagatgg agatgtcaac ggtcataagt tttctgtacg tggtgaaggc gaaggtgatg
      181 ctactaatgg caaactgact ctgaagttca tctgtacgac cggaaaattg ccagttccat
      241 ggcctacatt agtaacaaca ttaacctacg gagttcaatg cttttcaaga tacccagatc
      301 acatgaagca gcatgacttt ttcaaaagtg ctatgccaga aggttatgtg caggagagaa
      361 ctatctcctt caaagatgat ggtacttata agacaagagc agaggtaaag tttgaaggcg
      421 atacattggt taatagaatt gagcttaaag gcattgattt taaggaagat gggaatatcc
      481 tcggacataa acttgaatac aatttcaatt cacataatgt ttatatcaca gcagataaac
      541 aaaagaatgg tataaaagcg aactttaaga ttagacataa cgtggaagat ggtagcgtcc
      601 aattagccga ccattaccaa caaaacactc ctatcgggga cgggccagtt ctccttccag
      661 acaaccacta cctatcaacc caatctgttc tatctaaaga ccctaatgaa aaacgagatc
      721 acatggtctt gttagagttc gtgactgccg ctggtataac attaggaatg gacgaattgt
      781 acaaataagg cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat
      841 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
      901 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
      961 gtatgaggtc gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt
     1021 aatatagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg
     1081 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
     1141 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
     1201 gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg
     1261 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
     1321 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
     1381 acagttctca catcacatcc gaacataaac aaccatgaca gtcaacacta agacctatag
     1441 tgagagagca gaaactcatg cctcaccagt agcacagcga ttatttcgat taatggaact
     1501 gaagaaaacc aatttatgtg catcaattga cgttgatacc actaaggaat tccttgaatt
     1561 aattgataaa ttaggtcctt atgtatgctt aatcaagact catattgata taatcaatga
     1621 tttttcctat gaatccacta ttgaaccatt attagaactt tcacgtaaac atcaatttat
     1681 gatttttgaa gatagaaaat ttgctgatat tggtaatacc gtaaagaaac aatatattgg
     1741 tggagtttat aaaattagta gttgggcaga tattaccaat gctcatggtg tcactgggaa
     1801 tggagtggtt gaaggattaa aacagggagc taaagaaacc accaccaacc aagagccaag
     1861 agggttattg atgttagctg aattatcatc agtgggatca ttagcatatg gagaatattc
     1921 tcaaaaaact gttgaaattg ctaaatccga taaggaattt gttattggat ttattgccca
     1981 acgtgatatg ggtggccaag aagaaggatt tgattggctt attatgacac ctggagttgg
     2041 attagatgat aaaggtgatg gattaggaca acaatataga actgttgatg aagttgttag
     2101 cactggaact gatattatca ttgttggtag aggattgttt ggtaaaggaa gagatccaga
     2161 tattgaaggt aaaaggtata gaaatgctgg ttggaatgct tatttgaaaa agactggcca
     2221 attataatca gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag
     2281 tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt
     2341 cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc
     2401 aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca
     2461 gtgtcgaaaa cgagctcgaa ttcatcgaac gtgttttttt aatgtcccta accactcatt
     2521 cttacttctt tttgatgtgc tagt
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