##gff-version 3 ##sequence-region BBa_K1486035 1 1662 BBa_K1486035 . engineered_region 303 1662 . + 0 ID=BBa_K1486031;Name=BBa_K1486031 BBa_K1486035 . stop_codon 1464 1662 . + 0 ID=annotation2417787;Name=TEF terminator;Parent=BBa_K1486031 BBa_K1486035 . promoter 303 646 . + 0 ID=annotation2417785;Name=TEF promoter;Parent=BBa_K1486031 BBa_K1486035 . CDS 647 1459 . + 0 ID=annotation2417786;Name=Ura3;Parent=BBa_K1486031 BBa_K1486035 . terminator 115 302 . + 0 ID=BBa_K1486025;Name=BBa_K1486025 BBa_K1486035 . stop_codon 115 302 . + 0 ID=annotation2418776;Name=Adh1 terminator;Parent=BBa_K1486025 BBa_K1486035 . polypeptide_domain 1 39 . + 0 ID=BBa_K1486037;Name=BBa_K1486037 BBa_K1486035 . tag 1 39 . + 0 ID=annotation2417686;Name=13 amino acids linker;Parent=BBa_K1486037 BBa_K1486035 . tag 40 114 . + 0 ID=BBa_K1486034;Name=BBa_K1486034 BBa_K1486035 . tag 40 114 . + 0 ID=annotation2417784;Name=Yeast optimized sfGFP C-terminus;Parent=BBa_K1486034 >BBa_K1486035 ggcggtggctctggaggtggtgggtccggcggtggctctcgagatcacatggtcttgttagagttcgtgactgccgctg gtataacattaggaatggacgaattgtacaaataagcgaatttcttatgatttatgatttttattattaaataagttat aaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttc ctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgggacatggaggccca gaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacat ccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcaga cctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaagg ttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatcc gaacataaacaaccatgacagtcaacactaagacctatagtgagagagcagaaactcatgcctcaccagtagcacagcg attatttcgattaatggaactgaagaaaaccaatttatgtgcatcaattgacgttgataccactaaggaattccttgaa ttaattgataaattaggtccttatgtatgcttaatcaagactcatattgatataatcaatgatttttcctatgaatcca ctattgaaccattattagaactttcacgtaaacatcaatttatgatttttgaagatagaaaatttgctgatattggtaa taccgtaaagaaacaatatattggtggagtttataaaattagtagttgggcagatattaccaatgctcatggtgtcact gggaatggagtggttgaaggattaaaacagggagctaaagaaaccaccaccaaccaagagccaagagggttattgatgt tagctgaattatcatcagtgggatcattagcatatggagaatattctcaaaaaactgttgaaattgctaaatccgataa ggaatttgttattggatttattgcccaacgtgatatgggtggccaagaagaaggatttgattggcttattatgacacct ggagttggattagatgataaaggtgatggattaggacaacaatatagaactgttgatgaagttgttagcactggaactg atattatcattgttggtagaggattgtttggtaaaggaagagatccagatattgaaggtaaaaggtatagaaatgctgg ttggaatgcttatttgaaaaagactggccaattataatcagtactgacaataaaaagattcttgttttcaagaacttgt catttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcga catcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctata ctg