BBa_K1486053 1 BBa_K1486053 10 aa linker 2014-10-06T11:00:00Z 2015-05-08T01:10:42Z to edit to edit false false _1866_ 0 21042 9 Not in stock false to edit false EPFL iGem team 2014 BBa_K1486055 1 BBa_K1486055 CheY/CheZ + split fLuc 2014-10-06T11:00:00Z 2015-05-08T01:10:42Z to edit to edit false false _1866_ 0 21042 9 It's complicated false to edit false EPFL iGem team 2014 component2399886 1 BBa_B0034 component2399882 1 BBa_K569017 component2399879 1 BBa_I0500 component2399894 1 BBa_K1486053 component2399893 1 BBa_K629003 component2399895 1 BBa_K1486017 component2399883 1 BBa_K1486053 component2399881 1 BBa_B0034 component2399884 1 BBa_K1486016 annotation2399893 1 BBa_K629003 range2399893 1 2157 2801 annotation2399881 1 BBa_B0034 range2399881 1 1211 1222 annotation2399884 1 BBa_K1486016 range2399884 1 1671 2144 annotation2399886 1 BBa_B0034 range2399886 1 2145 2156 annotation2399882 1 BBa_K569017 range2399882 1 1223 1640 annotation2399894 1 BBa_K1486053 range2399894 1 2802 2831 annotation2399895 1 BBa_K1486017 range2399895 1 2832 4079 annotation2399879 1 BBa_I0500 range2399879 1 1 1210 annotation2399883 1 BBa_K1486053 range2399883 1 1641 1670 BBa_K569017 1 BBa_K569017 CheY 2011-09-26T11:00:00Z 2015-05-08T01:12:44Z ? Released HQ 2013 ? false false _737_ 0 9213 9 In stock false ? false David Golynskiy and Tyler Guinn BBa_K1486017 1 BBa_K1486017 fLuc[2] 2014-09-14T11:00:00Z 2015-05-08T01:10:42Z to edit This is the second part of the split Firefly luciferase. To be used with fLuc[1]. false false _1866_ 0 21042 9 Not in stock false to edit false EPFL iGem team 2014 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498842 1 PC range1498842 1 1043 1114 annotation1498843 1 AraC range1498843 1 1 1079 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K629003 1 cheZ cheZ, chemotaxis regulator, protein phosphatase for CheY 2011-10-02T11:00:00Z 2015-05-08T01:12:56Z genome of E. Coli BL21 plys strain Released HQ 2013 Polar localization is dependent on CheA-short. CheZ is a cytosolic phosphatase which functions in the chemotaxis signal transduction complex. Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P). false false _801_ 0 9022 9 In stock false how to control the proper expression of cheZ false Zilong WANG, Yi ZHENG annotation2154422 1 start range2154422 1 1 3 annotation2154425 1 CheZ range2154425 1 1 645 annotation2154421 1 fwd primer range2154421 1 1 20 annotation2154423 1 rev primer range2154423 1 624 645 annotation2154424 1 terminator range2154424 1 643 645 annotation2155668 1 TGA to TAA range2155668 1 643 645 BBa_K1486016 1 BBa_K1486016 fLuc[1] 2014-09-14T11:00:00Z 2015-05-08T01:10:42Z to edit This is the first part of the split Firefly luciferase. To be used with fLuc[2]. false false _1866_ 0 21042 9 Not in stock false to edit false EPFL iGem team 2014 BBa_K569017_sequence 1 cacctgcaaaaggagatggcggataaagaacttaaatttttggttgtggatgacttttccaccatgcgacgcatagtgcgtaacctgctgaaagagctgggattcaataatgttgaggaagcggaagatggcgtcgacgctctcaataagttgcaggcaggcggttatggatttgttatctccgactggaacatgcccaatatggatggcctggaattgctgaaaacaattcgtgcggatggcgcgatgtcggcattgccagtgttaatggtgactgcagaagcgaagaaagagaacatcattgctgcggcgcaagcgggggccagtggctatgtggtgaagccatttaccgccgcgacgctggaggaaaaactcaacaaaatctttgagaaactgggcatgtccctttttgcaggtg BBa_B0034_sequence 1 aaagaggagaaa BBa_K1486017_sequence 1 atggaggacgcgaaaaacatcaagaaaggcccagcaccgttctatccgctggaagatggtaccgccggtgaacaattgcacaaagcgatgaaacgttatgcgctggttccgggtaccatcgcattcacggacgcgcatatcgaggttaacatcacctatgccgagtacttcgagatgagcgttcgtctggccgaggcaatgaagcgttatggtttgaataccaatcatcgcatcgtcgtctgtagcgagaacagcttgcagtttttcatgccagttctgggtgcactgtttattggtgtggcagtggcgcctgcgaatgatatctacaacgagcgtgaactgttgaatagcatgaacatttcgcagccgacggttgtttttgtgtccaagaaaggtttgcagaagattctgaatgtgcaaaagaagttgccgatcattcagaaaatcatcatcatggacagcaagaccgattaccagggtttccaaagcatgtacacttttgtgacgagccacctgcctccgggttttaacgagtatgatttcgttccggaaagcttcgaccgtgataagacgattgcactgattatgaactcgtccggctctaccggcctgcctaaaggcgtggccctgccgcaccgtactgcatgtgtccgcttcagccacgcgcgtgatccgatcttcggtaaccaaatcattccggacaccgccattttgtctgtcgttccgtttcatcatggctttggtatgtttaccactctgggttacctgatctgtggtttccgtgttgttctgatgtaccgtttcgaagaagaactgttcctgcgtagcctgcaagattacaagatccaatccgcgctgctggtgccgacgctgtttagcttcttcgctaaatccaccctgattgacaaatatgacttgagcaacctgcacgaaatcgcgtcgggcggtgcgccgctgagcaaagaagtcggtgaggccgttgcgaaacgtttccatctgccgggcatccgtcagggttatggcctgacggaaacgaccagcgcgattttgattacccctgagggtgacgataaaccgggtgccgttggcaaggtggtaccgttctttgaggctaaagtcgtggacctggacacgggtaagactctgggtgtgaatcaacgtggcgagttgtgcgttcgtggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggc BBa_K629003_sequence 1 atgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattttaa BBa_K1486055_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcaaagaggagaaacacctgcaaaaggagatggcggataaagaacttaaatttttggttgtggatgacttttccaccatgcgacgcatagtgcgtaacctgctgaaagagctgggattcaataatgttgaggaagcggaagatggcgtcgacgctctcaataagttgcaggcaggcggttatggatttgttatctccgactggaacatgcccaatatggatggcctggaattgctgaaaacaattcgtgcggatggcgcgatgtcggcattgccagtgttaatggtgactgcagaagcgaagaaagagaacatcattgctgcggcgcaagcgggggccagtggctatgtggtgaagccatttaccgccgcgacgctggaggaaaaactcaacaaaatctttgagaaactgggcatgtccctttttgcaggtgggtggcggtggctctggaggtggtgggtccggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggctggctgcacagcggcgacctggcgtactgggatgaggatgaacactttttcatcgttggtcgcctgaagagcctgatcaagtacaagggctaccaggtggcaccggctgaactggagagcattctgttgcaacaccctaatatcttcgacgcgggtgttgcgggtctgcctgatgatgatgccggtgaattgccagctgcggtggtcgttctggaacacggtaaaacgatgactgaaaaagaaatcgtggactacgtcgcctcgcaggtgaccaccgctaaaaagttgcgtggtggcgtggtgtttgtggatgaagtgccgaaaggcctgacgggtaaacgtgatgcacgtaagattcgtgagatcttgatcaaggcgaagaaaggtggtaaatccaagttgtaaaaagaggagaaaatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattttaaggtggcggtggctctggaggtggtgggtccatggaggacgcgaaaaacatcaagaaaggcccagcaccgttctatccgctggaagatggtaccgccggtgaacaattgcacaaagcgatgaaacgttatgcgctggttccgggtaccatcgcattcacggacgcgcatatcgaggttaacatcacctatgccgagtacttcgagatgagcgttcgtctggccgaggcaatgaagcgttatggtttgaataccaatcatcgcatcgtcgtctgtagcgagaacagcttgcagtttttcatgccagttctgggtgcactgtttattggtgtggcagtggcgcctgcgaatgatatctacaacgagcgtgaactgttgaatagcatgaacatttcgcagccgacggttgtttttgtgtccaagaaaggtttgcagaagattctgaatgtgcaaaagaagttgccgatcattcagaaaatcatcatcatggacagcaagaccgattaccagggtttccaaagcatgtacacttttgtgacgagccacctgcctccgggttttaacgagtatgatttcgttccggaaagcttcgaccgtgataagacgattgcactgattatgaactcgtccggctctaccggcctgcctaaaggcgtggccctgccgcaccgtactgcatgtgtccgcttcagccacgcgcgtgatccgatcttcggtaaccaaatcattccggacaccgccattttgtctgtcgttccgtttcatcatggctttggtatgtttaccactctgggttacctgatctgtggtttccgtgttgttctgatgtaccgtttcgaagaagaactgttcctgcgtagcctgcaagattacaagatccaatccgcgctgctggtgccgacgctgtttagcttcttcgctaaatccaccctgattgacaaatatgacttgagcaacctgcacgaaatcgcgtcgggcggtgcgccgctgagcaaagaagtcggtgaggccgttgcgaaacgtttccatctgccgggcatccgtcagggttatggcctgacggaaacgaccagcgcgattttgattacccctgagggtgacgataaaccgggtgccgttggcaaggtggtaccgttctttgaggctaaagtcgtggacctggacacgggtaagactctgggtgtgaatcaacgtggcgagttgtgcgttcgtggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggc BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_K1486016_sequence 1 ggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggctggctgcacagcggcgacctggcgtactgggatgaggatgaacactttttcatcgttggtcgcctgaagagcctgatcaagtacaagggctaccaggtggcaccggctgaactggagagcattctgttgcaacaccctaatatcttcgacgcgggtgttgcgggtctgcctgatgatgatgccggtgaattgccagctgcggtggtcgttctggaacacggtaaaacgatgactgaaaaagaaatcgtggactacgtcgcctcgcaggtgaccaccgctaaaaagttgcgtggtggcgtggtgtttgtggatgaagtgccgaaaggcctgacgggtaaacgtgatgcacgtaagattcgtgagatcttgatcaaggcgaagaaaggtggtaaatccaagttgtaa BBa_K1486053_sequence 1 ggtggcggtggctctggaggtggtgggtcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z