BBa_K1487026
1
BBa_K1487026
Promoter + Guide RNA (gRNA) target for ctxB
2014-10-02T11:00:00Z
2015-05-08T01:10:43Z
Part was assembled using complementary primers to match a 20 base pair sequence in the ctxB coding region.
This part contains guide RNA (gRNA) that can target cholera toxin B (ctxB) gene under strong constitutive promoter J23119.
false
false
_1867_
0
22722
9
It's complicated
false
Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.
false
Samantha Bermudez
component2393188
1
BBa_J23119
component2393189
1
BBa_K1487005
annotation2393188
1
BBa_J23119
range2393188
1
1
35
annotation2393189
1
BBa_K1487005
range2393189
1
44
145
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K1487005
1
BBa_K1487005
Guide RNA (gRNA) target for ctxB
2014-10-01T11:00:00Z
2015-05-08T01:10:42Z
Part was assembled using complementary primers to match a 20 base pair sequence in the ctxB coding region.
This part contains guide RNA (gRNA) that can target cholera toxin B (acfB) gene.
false
false
_1867_
0
22722
9
Not in stock
false
Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.
false
Samantha Bermudez
BBa_K1487026_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagctatcttcagcatatgcaaagttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttt
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_K1487005_sequence
1
ctatcttcagcatatgcaaagttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z