BBa_K1487027
1
BBa_K1487027
Promoter + Guide RNA (gRNA) target for tcpA
2014-10-02T11:00:00Z
2015-05-08T01:10:43Z
Part was assembled using complementary primers to match a 20 base pair sequence in the tcpA coding region.
This part contains guide RNA (gRNA) that can target toxin coregulated pilus A (tcp) gene under strong constitutive promoter J23119.
false
false
_1867_
0
22722
9
It's complicated
false
Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.
false
Samantha Bermudez
component2393194
1
BBa_K1487006
component2393193
1
BBa_J23119
annotation2393193
1
BBa_J23119
range2393193
1
1
35
annotation2393194
1
BBa_K1487006
range2393194
1
44
145
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K1487006
1
BBa_K1487006
Guide RNA (gRNA) target for tcpA
2014-10-01T11:00:00Z
2015-05-08T01:10:42Z
Part was assembled using complementary primers to match a 20 base pair sequence in the tcpA coding region.
This part contains guide RNA (gRNA) that can target toxin coregulated pilus A (tcpA) gene.
false
false
_1867_
0
22722
9
Not in stock
false
Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.
false
Samantha Bermudez
BBa_K1487006_sequence
1
ctcgaagtgatcatcgttctgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttt
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_K1487027_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagctcgaagtgatcatcgttctgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z