BBa_K1487069 1 BBa_K1487069 eYFP Reporter for tcpA gRNA target with strong promoter. 2014-10-05T11:00:00Z 2015-05-08T01:10:43Z Part was assembled using complementary primers to match a 20 base pair sequence in the tcpA coding region. This part contains a 20bp complementary target sequence for toxin coregulated pilus A (tcpA) that can be targeted by the gRNA from Part:BBa_K1487027. The target sequence is upstream from ribosome binding site Part:BBa_B0034 and a coding region for enhanced yellow fluorescent protein (Part:BBa_E0030). This reporter was placed under a strong constitutive reporter (Part:BBa_J23119). false false _1867_ 0 22722 9 It's complicated false Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence. false Samantha Bermudez component2397514 1 BBa_K1487048 component2397513 1 BBa_J23119 annotation2397514 1 BBa_K1487048 range2397514 1 44 807 annotation2397513 1 BBa_J23119 range2397513 1 1 35 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_K1487048 1 BBa_K1487048 Target sequence for tcpA + RBS + eYFP 2014-10-05T11:00:00Z 2015-05-08T01:10:43Z Part was assembled using complementary primers to match a 20 base pair sequence in the tcpA coding region. This part contains a 20bp complementary target sequence for toxin coregulated pilus A (tcpA) that can be targeted by the gRNA from Part:BBa_K1487027. The target sequence is upstream from ribosome binding site Part:BBa_B0034 and a coding region for enhanced yellow fluorescent protein (Part:BBa_E0030). false false _1867_ 0 22722 9 Not in stock false Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence. false Samantha Bermudez BBa_K1487069_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagctcgaagtgatcatcgttctaggaaagaggagaaaactagaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_K1487048_sequence 1 ctcgaagtgatcatcgttctaggaaagaggagaaaactagaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z