LOCUS BBa_K1489004 1094 bp DNA linear 22-Nov-2024 DEFINITION pBAD-RBS-OmpA-Bovine Galectin-1-B0012 Terminator ACCESSION BBa_K1489004 VERSION BBa_K1489004.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 promoter 1..130 /label=BBa_K206000 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 misc_feature 162..164 /label=ATG start misc_feature 159..164 /label=NdeI site misc_feature 617..622 /label=SacI site CDS 597..622 /label=Linker CDS 162..596 /label=Lpp-OmpA CDS 159..622 /label=BBa_K1489002 misc_feature 629..631 /label=ATG Start misc_feature 1043..1045 /label=TGA Stop CDS 629..1045 /label=BBa_K1489001 stem_loop 1061..1080 /label=T7 TE misc_feature 1087..1087 /label=stop polyA_site 1081..1094 /label=polya misc_feature 1054..1094 /label=BBa_B0012 terminator 1054..1094 /label=BBa_B0012 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagagca tatgaaagct actaaactgg 181 tactgggcgc ggtaatcctg ggttctactc tgctggcagg ttgctccagc aacgctaaaa 241 tcgatcaggg aattaacccg tatgttggct ttgaaatggg ttacgactgg ttaggtcgta 301 tgccgtacaa aggcagcgtt gaaaacggtg catacaaagc tcagggcgtt caactgaccg 361 ctaaactggg ttacccaatc actgacgacc tggacatcta cactcgtctg ggtggcatgg 421 tatggcgtgc agacactaaa tccaacgttt atggtaaaaa ccacgacacc ggcgtttctc 481 cggtcttcgc tggcggtgtt gagtacgcga tcactcctga aatcgctacc cgtctggaat 541 accagtggac caacaacatc ggtgacgcac acaccatcgg cactcgtccg gacaacggcg 601 gaggttctgg aggagggagc tctactagat ggcgtcgggg cttgttgcgt ccaatctgaa 661 tctgaaacca ggtgaaagct tacgcgtccg cggcgaagta gccgcagatg cgaaatcttt 721 tctcttgaac ctgggcaagg acgataataa tctggctttg catttcaacc cgcgtttcaa 781 cgctcatggc gacgtgaaca caatcgtgtg taattccaag gatgctggtg cttggggtgc 841 agaacagcgt gagtccgcgt tcccgtttca accgggctca gttgtggaag tcaccatcag 901 cttcaaccag accgatctca ccattaaact gccggatggc tacgagttta agtttccaaa 961 ccgtttaaac ctcgaggcca tcaactatct gtccgcgggg ggcgacttca agattaaaag 1021 cgtgcaccat catcaccacc attgatacta gagtcacact ggctcacctt cgggtgggcc 1081 tttctgcgtt tata //