LOCUS       BBa_K1491011             966 bp    DNA     linear       26-Jan-2026
DEFINITION  WTLac-RBS-GFP-His
ACCESSION   BBa_K1491011
VERSION     BBa_K1491011.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             229..942
                     /label=eGFP
     misc_feature    943..960
                     /label=6x His
     CDS             229..966
                     /label=BBa_K1491002
     misc_feature    209..966
                     /label=BBa_K1491003
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagaggt gagcaagggc
      241 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      301 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      361 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      421 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      481 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      541 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      601 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      661 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
      721 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      781 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      841 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      901 accgccgccg ggatcactct cggcatggac gagctgtaca agcatcatca ccaccatcac
      961 taataa
//