LOCUS       BBa_K1491012             967 bp    DNA     linear       26-Jan-2026
DEFINITION  WTLac-RBS-YFP-His
ACCESSION   BBa_K1491012
VERSION     BBa_K1491012.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..943
                     /label=BBa_K864100
     misc_feature    944..961
                     /label=6x His
     CDS             227..967
                     /label=BBa_K1491004
     misc_feature    209..967
                     /label=BBa_K1491005
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ttagcaaggg
      241 cgaagaactt tttacaggcg tagtaccgat cttagttgaa ttagacggcg acgttaacgg
      301 tcataagttt agcgtgagcg gtgagggtga aggtgacgca acttacggca agctgaccct
      361 gaagctgatt tgcacgacgg gtaagctgcc ggtcccgtgg cctaccctgg tcacgacctt
      421 gggttatggc gttcagtgtt tcgcgcgtta tccggaccac atgaaacaac acgatttctt
      481 taagagcgcg atgccagaag gctatgtgca ggagcgtacg atctttttca aagacgacgg
      541 taactacaag acgcgtgccg aagtcaaatt cgaaggcgac accctggtga atcgcattga
      601 gctgaagggt attgatttca aagaggatgg caatatcctg ggtcacaagc tggagtacaa
      661 ttacaattcc cacaacgttt acatcaccgc agataaacag aaaaatggca tcaaagcgaa
      721 tttcaaaatc cgtcacaaca ttgaggacgg tggtgttcaa ctggcggatc attaccagca
      781 aaacaccccg attggtgacg gtccggtcct gttgccggat aaccattatc tgtcttacca
      841 aagcaaactg agcaaagatc cgaacgagaa gcgcgaccac atggtgctgc tggagtttgt
      901 gaccgctgcc ggtattaccc tgggtatgga tgagctgtat aaacatcatc accaccatca
      961 ctaataa
//