LOCUS       BBa_K1491013             973 bp    DNA     linear       22-Dec-2024
DEFINITION  WTLac-RBS-OFP-His
ACCESSION   BBa_K1491013
VERSION     BBa_K1491013.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..949
                     /label=BBa_E2050
     misc_feature    950..967
                     /label=6x His
     CDS             227..973
                     /label=BBa_K1491006
     misc_feature    209..973
                     /label=BBa_K1491007
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ttagtaaagg
      241 agaagaaaac aatatggcaa tcataaaaga atttatgaga ttcaaagtca gaatggaagg
      301 ttctgtaaat ggtcacgagt tcgaaataga aggggaaggt gaaggtagac cctatgaagg
      361 ctttcaaacg gctaaattaa aagttaccaa gggtggacca ttgccctttg cttgggatat
      421 cctgtctcct cagttcactt atggtagtaa agcctatgtt aagcatcctg ctgatattcc
      481 tgattacttc aagttgagtt ttccagaagg tttcaaatgg gagagagtta tgaattttga
      541 agatggcgga gttgtgacag tgacacaaga ctcctcactt caagacggtg agtttattta
      601 caaggtaaaa ctacgtggca ctaactttcc gtcggatgga ccagtcatgc aaaaaaagac
      661 gatgggttgg gaggcttcat ctgagcgaat gtatccagaa gatggggcac taaagggcga
      721 aattaagatg aggctcaaat taaaggatgg tggacattat acctcggaag tgaaaactac
      781 ctataaagcc aaaaagccag ttcaattacc tggtgcatac attgttggca ttaagttgga
      841 catcacaagc cacaatgaag attatacaat agtagagcag tacgaacgcg cggaaggtag
      901 gcattctact ggaggcatgg atgaactata caaaggttct ggtaccgcac atcatcacca
      961 ccatcactaa taa
//