LOCUS       BBa_K1491016             967 bp    DNA     linear       22-Dec-2024
DEFINITION  Lac Inducible Codon Optimized KillerRed
ACCESSION   BBa_K1491016
VERSION     BBa_K1491016.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    944..961
                     /label=6x His Tag
     CDS             227..967
                     /label=BBa_K1491015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg gcagcgaagg
      241 cggtcccgcg ctgtttcaaa gtgacatgac ttttaaaatc tttattgatg gagaagttaa
      301 tggacaaaag tttactattg tcgcagacgg ctcatccaag tttccgcatg gggattttaa
      361 cgtccatgca gtatgcgaga cggggaaatt accgatgagc tggaaaccga tctgtcatct
      421 gatccagtat ggcgaaccgt tctttgcacg ttatcctgat ggcatctctc atttcgccca
      481 agaatgcttc ccggaaggac tttctattga tcgtaccgtt cgctttgaga atgacggtac
      541 catgacttct catcacacct acgaactgga tgacacctgc gtggtctctc gtatcacggt
      601 gaattgtgat ggttttcagc cggatggccc gatcatgcgt gaccaattgg ttgatattct
      661 cccgaatgag acacatatgt tcccgcatgg gccgaacgcc gtgcgccagc tggcctttat
      721 cggttttaca accgcagatg gaggtctgat gatgggtcat tttgatagta aaatgacgtt
      781 taacggctcc cgtgcgatcg agattcccgg tcctcacttc gttaccatca tcacgaaaca
      841 gatgcgcgat acttcggata aacgcgatca tgtatgtcaa cgcgaggtgg cgtatgccca
      901 ttccgtgccg cgcattacat ctgcgatcgg cagcgatgaa gatcatcatc accaccatca
      961 ctaataa
//