BBa_K1491020 1 BBa_K1491020 Constitutive Codon Optimized Killer Red 2014-09-14T11:00:00Z 2015-05-08T01:10:44Z Codon Optimized Killer Red is BBa_K1491015, the promoter is BBa_J23100 and the RBS is BBa_B0034 Codon optimized version of superoxide generator Killer Red (BBa_K1491015) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034). false false _1871_ 0 22521 9 It's complicated false Constitutive version of Codon Optimized Killer Red for performing photobleaching experiments. false Danielle Peters component2383503 1 BBa_B0034 component2383501 1 BBa_J23100 component2383505 1 BBa_K1491015 annotation2383501 1 BBa_J23100 range2383501 1 1 35 annotation2383503 1 BBa_B0034 range2383503 1 44 55 annotation2383505 1 BBa_K1491015 range2383505 1 62 802 BBa_K1491015 1 BBa_K1491015 Codon Optimized KillerRed 2014-08-03T11:00:00Z 2015-05-08T01:10:44Z This part was codon optimized from the original KillerRed (BBa_K1184000) This part is the codon optimized version of KillerRed (BBa_K1184000). It has been optimized for E. coli by eliminating the rare codons. false false _1871_ 0 22521 9 In stock false Increase the functionality of KillerRed through codon optimization. false Danielle Peters annotation2380344 1 6x His Tag range2380344 1 718 735 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0034_sequence 1 aaagaggagaaa BBa_K1491015_sequence 1 atgggcagcgaaggcggtcccgcgctgtttcaaagtgacatgacttttaaaatctttattgatggagaagttaatggacaaaagtttactattgtcgcagacggctcatccaagtttccgcatggggattttaacgtccatgcagtatgcgagacggggaaattaccgatgagctggaaaccgatctgtcatctgatccagtatggcgaaccgttctttgcacgttatcctgatggcatctctcatttcgcccaagaatgcttcccggaaggactttctattgatcgtaccgttcgctttgagaatgacggtaccatgacttctcatcacacctacgaactggatgacacctgcgtggtctctcgtatcacggtgaattgtgatggttttcagccggatggcccgatcatgcgtgaccaattggttgatattctcccgaatgagacacatatgttcccgcatgggccgaacgccgtgcgccagctggcctttatcggttttacaaccgcagatggaggtctgatgatgggtcattttgatagtaaaatgacgtttaacggctcccgtgcgatcgagattcccggtcctcacttcgttaccatcatcacgaaacagatgcgcgatacttcggataaacgcgatcatgtatgtcaacgcgaggtggcgtatgcccattccgtgccgcgcattacatctgcgatcggcagcgatgaagatcatcatcaccaccatcactaataa BBa_K1491020_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgggcagcgaaggcggtcccgcgctgtttcaaagtgacatgacttttaaaatctttattgatggagaagttaatggacaaaagtttactattgtcgcagacggctcatccaagtttccgcatggggattttaacgtccatgcagtatgcgagacggggaaattaccgatgagctggaaaccgatctgtcatctgatccagtatggcgaaccgttctttgcacgttatcctgatggcatctctcatttcgcccaagaatgcttcccggaaggactttctattgatcgtaccgttcgctttgagaatgacggtaccatgacttctcatcacacctacgaactggatgacacctgcgtggtctctcgtatcacggtgaattgtgatggttttcagccggatggcccgatcatgcgtgaccaattggttgatattctcccgaatgagacacatatgttcccgcatgggccgaacgccgtgcgccagctggcctttatcggttttacaaccgcagatggaggtctgatgatgggtcattttgatagtaaaatgacgtttaacggctcccgtgcgatcgagattcccggtcctcacttcgttaccatcatcacgaaacagatgcgcgatacttcggataaacgcgatcatgtatgtcaacgcgaggtggcgtatgcccattccgtgccgcgcattacatctgcgatcggcagcgatgaagatcatcatcaccaccatcactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z