BBa_K1491021
1
BBa_K1491021
Constitutive Codon Optimized Super Nova
2014-09-14T11:00:00Z
2015-05-08T01:10:44Z
Codon Optimized Killer Red is BBa_K1491017, the promoter is BBa_J23100 and the RBS is BBa_B0034
Codon optimized version of the monomeric superoxide generator Super Nova (BBa_K1491017) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034). Super Nova is the monomeric form of Killer Red (BBa_K1184000).
false
false
_1871_
0
22521
9
It's complicated
false
Constitutive version of Codon Optimized Super Nova for performing photobleaching experiments.
false
Danielle Peters
component2383508
1
BBa_B0034
component2383506
1
BBa_J23100
component2383510
1
BBa_K1491017
annotation2383506
1
BBa_J23100
range2383506
1
1
35
annotation2383510
1
BBa_K1491017
range2383510
1
62
802
annotation2383508
1
BBa_B0034
range2383508
1
44
55
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1491017
1
BBa_K1491017
Codon Optimized Supernova
2014-08-03T11:00:00Z
2015-05-08T01:10:44Z
The original KillerRed is BBa_K11840000.
Supernova is the monomeric version of KillerRed (BBa_K1184000). It has been codon optimized for E. coli.
false
false
_1871_
0
22521
9
In stock
false
Monomeric version of KillerRed
false
Danielle Peters
annotation2380356
1
6x His Tag
range2380356
1
718
735
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1491017_sequence
1
atgggcagcgaagtgggcccagcgttattccagagtgacatgacctttaagatcttcatcgacggtgaagtcaacggtcaaaaatttactattgtggctgacggctccagtaaatttccacatggtgatttcaacgtgcacgccgtatgtgaaacgggcaaactccctatgtcatggaaaccgatttgccacttgattcagtacggtgaaccgttttttgcccgctatcctgatgggatctcgcatttcgcgcaggagtgtttcccggaaggcctgtcaatcgatcgcacagtgcgctttgaaaacgatggcacgatgacgtctcatcacacatacgaattagatgatacttgtgtagtctctcgtattaccgtgaactgcgatgggttccagccggatggcccaatcatgcgtgatcagttagtcgacattctcccgtcggaaactcacatgtttccgcacggacctaatgctgtgcgccaaacagcgacgatcggctttaccaccgcggacggtggtaaaatgatgggccattttgatagcaaaatgacgtttaacggcagccgcgctattgagattccgggtccacatttcgtgactatcatcactaaacagacgcgtgatacttcggacaaacgcgaccatgtgtgtcaacgcgaggtggcgtatgctcacagcgtaccgcgcattaccagcgctattggtagcgacgaagatcatcatcaccaccatcactaataa
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1491021_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgggcagcgaagtgggcccagcgttattccagagtgacatgacctttaagatcttcatcgacggtgaagtcaacggtcaaaaatttactattgtggctgacggctccagtaaatttccacatggtgatttcaacgtgcacgccgtatgtgaaacgggcaaactccctatgtcatggaaaccgatttgccacttgattcagtacggtgaaccgttttttgcccgctatcctgatgggatctcgcatttcgcgcaggagtgtttcccggaaggcctgtcaatcgatcgcacagtgcgctttgaaaacgatggcacgatgacgtctcatcacacatacgaattagatgatacttgtgtagtctctcgtattaccgtgaactgcgatgggttccagccggatggcccaatcatgcgtgatcagttagtcgacattctcccgtcggaaactcacatgtttccgcacggacctaatgctgtgcgccaaacagcgacgatcggctttaccaccgcggacggtggtaaaatgatgggccattttgatagcaaaatgacgtttaacggcagccgcgctattgagattccgggtccacatttcgtgactatcatcactaaacagacgcgtgatacttcggacaaacgcgaccatgtgtgtcaacgcgaggtggcgtatgctcacagcgtaccgcgcattaccagcgctattggtagcgacgaagatcatcatcaccaccatcactaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z