LOCUS BBa_K1491027 4258 bp DNA linear 28-May-2024 DEFINITION T7 RNA Polymerase with Dead ER Intein ACCESSION BBa_K1491027 VERSION BBa_K1491027.1 FEATURES Location/Qualifiers CDS 1..1473 /label=BBa_K1491022 misc_feature 1482..2096 /label=N term Intein misc_feature 2097..2854 /label=Estrogen Receptor misc_feature 2855..3056 /label=C term Intein misc_feature 3054..3056 /label=Dead Mutation CDS 1482..3056 /label=BBa_K1491026 misc_feature 4241..4258 /label=6x His Tag CDS 3065..4258 /label=BBa_K1491023 ORIGIN 1 atgaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg 61 ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt ggcccttgag 121 catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa 181 gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct actccctaag 241 atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caagcgcccg 301 acagccttcc agttcctgca agaaatcaag ccggaagccg tagcgtacat caccattaag 361 accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca 421 atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag 481 cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaagaaa 541 gcatttatgc aagttgtcga ggctgacatg ctctctaagg gtctactcgg tggcgaggcg 601 tggtcttcgt ggcataagga agactctatt catgtaggag tacgctgcat cgagatgctc 661 attgagtcaa ccggaatggt tagcttacac cgccaaaatg ctggcgtagt aggtcaagac 721 tctgagacta tcgaactcgc acctgaatac gctgaggcta tcgcaacccg tgcaggtgcg 781 ctggctggca tctctccgat gttccaacct tgcgtagttc ctcctaagcc gtggactggc 841 attactggtg gtggctattg ggctaacggt cgtcgtcctc tggcgctggt gcgtactcac 901 agtaagaaag cactgatgcg ctacgaagac gtttacatgc ctgaggtgta caaagcgatt 961 aacattgcgc aaaacaccgc atggaaaatc aacaagaaag tcctagcggt cgccaacgta 1021 atcaccaagt ggaagcattg tccggtcgag gacatccctg cgattgagcg tgaagaactc 1081 ccgatgaaac cggaagacat cgacatgaat cctgaggctc tcaccgcgtg gaaacgtgct 1141 gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag ccttgagttc 1201 atgcttgagc aagccaataa gtttgctaac cataaggcca tctggttccc ttacaacatg 1261 gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa cgatatgacc 1321 aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta ctactggctg 1381 aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc cgttccctga gcgcatcaag 1441 ttcattgagg aaaaccacga gaacatcatg gcttactaga gtgctttgcc aagggtacca 1501 atgttttaat ggcggatggg tctattgaat gtattgagaa cattgaggtt ggtaataagg 1561 tcatgggtaa agatggcaga cctcgtgagg taattaaatt gcccagagga agtgaaacta 1621 tgtacagtgt cgtgcagaaa agtcaacaca gagcccataa aagtgactca agtcgtgaaa 1681 tgccagaatt actcaagttc acgtgtaatg cgactcatga gttggttgtt agaacacctc 1741 gtagtgtccg ccgtttgtct cgtaccatta agggtgtcga atattttgaa gttattactt 1801 ttgagatggg tcaaaagaag gcccccgacg gtagaattgt tgagcttgtc aaggaagttt 1861 caaagagcta tccagtatcc gagggtcctg agagagctaa cgaattggta gaatcctata 1921 gaaaggcttc aaataaagct tattttgagt ggactattga ggccagggat ctttctctgt 1981 tgggttccca tgttcgtaaa gctacctacc agacttacgc tccaattctt tatgagaatg 2041 accacttttt cgactacatg caaaaaagta agtttcatct caccattgaa ggtccatcta 2101 agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg 2161 ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt gaggcttcga 2221 tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc aactgggcga 2281 agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt ctcgaatgtg 2341 cctggctaga gatcctgatg attggtctcg tctggcgctc catggagcac ccagggaagc 2401 tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg 2461 tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg aatctccagg 2521 gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg tacacatttc 2581 tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc ctggacaaga 2641 tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctccag cagcagcacc 2701 agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt aacaaaggca 2761 tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg 2821 agatgctgga cgcccaccgc ctacatgcgc ccaccggtga tgttttgctt aacgttcttt 2881 ctaagtgtgc cggctctaaa aaattcaggc ctgctcccgt cgctactttt gtacgtgagt 2941 gccagggatt ttatttcgag ttacaagaat taaaggaaaa cgattattac ggaattacct 3001 tatctgatga ttctgatcat cagtttttgc ttgccaacca ggttgtcgtc catcagtact 3061 agagtgcgct aagtctccac tggagaacac ttggtgggct gagcaagatt ctccgttctg 3121 cttccttgcg ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg 3181 ctcccttccg ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct 3241 ccgagatgag gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat 3301 ctacgggatt gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac 3361 cgataacgaa gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa 3421 gctgggcact aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac 3481 taagcgttca gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt 3541 gctggaagat accattcagc cagctattga ttccggcaag ggtctgatgt tcactcagcc 3601 gaatcaggct gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt 3661 agctgcggtt gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt 3721 caaagataag aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc 3781 tgatggtttc cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat 3841 gttcctcggt cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga 3901 tgcacacaaa caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca 3961 ccttcgtaag actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat 4021 tcacgactcc ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga 4081 aactatggtt gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc 4141 tgaccagttg cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt 4201 gaacctccgt gacatcttag agtcggactt cgcgttcgcg catcatcacc accatcac //