LOCUS BBa_K1491031 4319 bp DNA linear 22-Nov-2024 DEFINITION J23115-B0034-T7 RNA Polymerase with Dead ER Intein ACCESSION BBa_K1491031 VERSION BBa_K1491031.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23115 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..1534 /label=BBa_K1491022 misc_feature 1543..2157 /label=N term Intein misc_feature 2158..2915 /label=Estrogen Receptor misc_feature 2916..3117 /label=C term Intein misc_feature 3115..3117 /label=Dead Mutation CDS 1543..3117 /label=BBa_K1491026 misc_feature 4302..4319 /label=6x His Tag CDS 3126..4319 /label=BBa_K1491023 CDS 62..4319 /label=BBa_K1491027 ORIGIN 1 tttatagcta gctcagccct tggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgaacacg attaacatcg ctaagaacga cttctctgac atcgaactgg ctgctatccc 121 gttcaacact ctggctgacc attacggtga gcgtttagct cgcgaacagt tggcccttga 181 gcatgagtct tacgagatgg gtgaagcacg cttccgcaag atgtttgagc gtcaacttaa 241 agctggtgag gttgcggata acgctgccgc caagcctctc atcactaccc tactccctaa 301 gatgattgca cgcatcaacg actggtttga ggaagtgaaa gctaagcgcg gcaagcgccc 361 gacagccttc cagttcctgc aagaaatcaa gccggaagcc gtagcgtaca tcaccattaa 421 gaccactctg gcttgcctaa ccagtgctga caatacaacc gttcaggctg tagcaagcgc 481 aatcggtcgg gccattgagg acgaggctcg cttcggtcgt atccgtgacc ttgaagctaa 541 gcacttcaag aaaaacgttg aggaacaact caacaagcgc gtagggcacg tctacaagaa 601 agcatttatg caagttgtcg aggctgacat gctctctaag ggtctactcg gtggcgaggc 661 gtggtcttcg tggcataagg aagactctat tcatgtagga gtacgctgca tcgagatgct 721 cattgagtca accggaatgg ttagcttaca ccgccaaaat gctggcgtag taggtcaaga 781 ctctgagact atcgaactcg cacctgaata cgctgaggct atcgcaaccc gtgcaggtgc 841 gctggctggc atctctccga tgttccaacc ttgcgtagtt cctcctaagc cgtggactgg 901 cattactggt ggtggctatt gggctaacgg tcgtcgtcct ctggcgctgg tgcgtactca 961 cagtaagaaa gcactgatgc gctacgaaga cgtttacatg cctgaggtgt acaaagcgat 1021 taacattgcg caaaacaccg catggaaaat caacaagaaa gtcctagcgg tcgccaacgt 1081 aatcaccaag tggaagcatt gtccggtcga ggacatccct gcgattgagc gtgaagaact 1141 cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct ctcaccgcgt ggaaacgtgc 1201 tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct cgccgtatca gccttgagtt 1261 catgcttgag caagccaata agtttgctaa ccataaggcc atctggttcc cttacaacat 1321 ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac ccgcaaggta acgatatgac 1381 caaaggactg cttacgctgg cgaaaggtaa accaatcggt aaggaaggtt actactggct 1441 gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt ccgttccctg agcgcatcaa 1501 gttcattgag gaaaaccacg agaacatcat ggcttactag agtgctttgc caagggtacc 1561 aatgttttaa tggcggatgg gtctattgaa tgtattgaga acattgaggt tggtaataag 1621 gtcatgggta aagatggcag acctcgtgag gtaattaaat tgcccagagg aagtgaaact 1681 atgtacagtg tcgtgcagaa aagtcaacac agagcccata aaagtgactc aagtcgtgaa 1741 atgccagaat tactcaagtt cacgtgtaat gcgactcatg agttggttgt tagaacacct 1801 cgtagtgtcc gccgtttgtc tcgtaccatt aagggtgtcg aatattttga agttattact 1861 tttgagatgg gtcaaaagaa ggcccccgac ggtagaattg ttgagcttgt caaggaagtt 1921 tcaaagagct atccagtatc cgagggtcct gagagagcta acgaattggt agaatcctat 1981 agaaaggctt caaataaagc ttattttgag tggactattg aggccaggga tctttctctg 2041 ttgggttccc atgttcgtaa agctacctac cagacttacg ctccaattct ttatgagaat 2101 gaccactttt tcgactacat gcaaaaaagt aagtttcatc tcaccattga aggtccatct 2161 aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat 2221 gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag tgaggcttcg 2281 atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg 2341 aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct tctcgaatgt 2401 gcctggctag agatcctgat gattggtctc gtctggcgct ccatggagca cccagggaag 2461 ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg 2521 gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctccag 2581 ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt 2641 ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag 2701 atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctcca gcagcagcac 2761 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc 2821 atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg 2881 gagatgctgg acgcccaccg cctacatgcg cccaccggtg atgttttgct taacgttctt 2941 tctaagtgtg ccggctctaa aaaattcagg cctgctcccg tcgctacttt tgtacgtgag 3001 tgccagggat tttatttcga gttacaagaa ttaaaggaaa acgattatta cggaattacc 3061 ttatctgatg attctgatca tcagtttttg cttgccaacc aggttgtcgt ccatcagtac 3121 tagagtgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat tctccgttct 3181 gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg agctataact 3241 gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc tccgcgatgc 3301 tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca 3361 tctacgggat tgttgctaag aaagtcaacg agattctaca agcagacgca atcaatggga 3421 ccgataacga agtagttacc gtgaccgatg agaacactgg tgaaatctct gagaaagtca 3481 agctgggcac taaggcactg gctggtcaat ggctggctta cggtgttact cgcagtgtga 3541 ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag 3601 tgctggaaga taccattcag ccagctattg attccggcaa gggtctgatg ttcactcagc 3661 cgaatcaggc tgctggatac atggctaagc tgatttggga atctgtgagc gtgacggtgg 3721 tagctgcggt tgaagcaatg aactggctta agtctgctgc taagctgctg gctgctgagg 3781 tcaaagataa gaagactgga gagattcttc gcaagcgttg cgctgtgcat tgggtaactc 3841 ctgatggttt ccctgtgtgg caggaataca agaagcctat tcagacgcgc ttgaacctga 3901 tgttcctcgg tcagttccgc ttacagccta ccattaacac caacaaagat agcgagattg 3961 atgcacacaa acaggagtct ggtatcgctc ctaactttgt acacagccaa gacggtagcc 4021 accttcgtaa gactgtagtg tgggcacacg agaagtacgg aatcgaatct tttgcactga 4081 ttcacgactc cttcggtacc attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg 4141 aaactatggt tgacacatat gagtcttgtg atgtactggc tgatttctac gaccagttcg 4201 ctgaccagtt gcacgagtct caattggaca aaatgccagc acttccggct aaaggtaact 4261 tgaacctccg tgacatctta gagtcggact tcgcgttcgc gcatcatcac caccatcac //